G-quadruplex functionalized nano mesoporous silica for assay of the DNA methyltransferase activity

Pei, Lei; Pang, Junling; Yin, Huanshun; Ai, Shiyun

doi:10.1016/j.aca.2015.04.023

Cited by 20 publications

(3 citation statements)

References 48 publications

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“…Recently, the mesoporous silica nanoparticle (MSN) has widely been used as a nanocarrier due to its high surface area, tunable pore structure and modifiable surface [ 74 , 75 ]. Fan et al proposed a MSN-based controlled release system with acid cleavable linkage for quantitative analysis of the prostate specific antigen [ 76 ].…”

Section: Signal Enhancement Via Labeling Techniquesmentioning

confidence: 99%

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Cho

Lee

Kim

et al. 2018

Sensors

192

115

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An electrochemical immunosensor employs antibodies as capture and detection means to produce electrical charges for the quantitative analysis of target molecules. This sensor type can be utilized as a miniaturized device for the detection of point-of-care testing (POCT). Achieving high-performance analysis regarding sensitivity has been one of the key issues with developing this type of biosensor system. Many modern nanotechnology efforts allowed for the development of innovative electrochemical biosensors with high sensitivity by employing various nanomaterials that facilitate the electron transfer and carrying capacity of signal tracers in combination with surface modification and bioconjugation techniques. In this review, we introduce novel nanomaterials (e.g., carbon nanotube, graphene, indium tin oxide, nanowire and metallic nanoparticles) in order to construct a high-performance electrode. Also, we describe how to increase the number of signal tracers by employing nanomaterials as carriers and making the polymeric enzyme complex associated with redox cycling for signal amplification. The pros and cons of each method are considered throughout this review. We expect that these reviewed strategies for signal enhancement will be applied to the next versions of lateral-flow paper chromatography and microfluidic immunosensor, which are considered the most practical POCT biosensor platforms.

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Section: Signal Enhancement Via Labeling Techniquesmentioning

confidence: 99%

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Cho

Lee

Kim

et al. 2018

Sensors

192

115

View full text Add to dashboard Cite

show abstract

“…142 However, traditional methods for DNA methyltransferase activity assay rely on radioisotope materials. 109 Therefore, radioisotope-free approaches have been developed, including electrochemical, [109][110][111][112][113][114][115][116][117][118][119] electrochemiluminescence, [120][121][122] photo-electrochemical, 123 fluorometric, [124][125][126][127][128][129][130][131][132][133][134][135] surface-enhanced Raman scattering (SERS), [136][137][138] and circular dichroism (CD) spectroscopy, 139 and surface plasmon resonance 140 based DNA methyltransferase measurement techniques have recently been proposed as summarized in Table 2 to realize simple, quick and easy monitoring.…”

Section: Dna Methyltransferase Activitymentioning

confidence: 99%

Microfluidic platforms for DNA methylation analysis

Kurita

Niwa

2016

Lab Chip

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In the field of genetics, epigenetics is the study of changes in gene expression without any change in DNA sequences. Chemical base modification in DNA by DNA methyltransferase, and specifically methylation, has been well studied as the main mechanism of epigenetics. Therefore, the determination of DNA methylation of, for example, 5'-methylcytosine in the CpG sequence in mammals has attracted attention because it should prove valuable in a wide range of research fields including diagnosis, drug discovery, and therapy. Methylated DNA bases and DNA methyltransferase activity are analyzed using conventional methods; however, these methods are time-consuming and require complex multiple operations. Therefore, new methods and devices for DNA methylation analysis are now being actively developed. Furthermore, microfluidic technology has also been applied to DNA methylation analysis because the microfluidic platform offers the promising advantage of making it possible to perform thousands of DNA methylation reactions in small reaction volumes, resulting in a high-throughput analysis with high sensitivity. This review discusses epigenetics and the microfluidic platforms developed for DNA methylation analysis.

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“…Among them, electrochemistry was the most attractive method for MTase activity detection owing to its remarkable features of high sensitivity, inexpensive instrument, low cost and portability [34]. Ai recently employed a new type of DNA functionalized nano/mesoporous silica to detect DNA MTase activity with G-quadruplex as a lock for electrochemical signal molecule releasing [31]. Our group designed an electrochemical method for sensitive detection of DNA methylation and MTase activity based on the deposition of polyaniline catalyzed by HRP-mimicking DNAzyme [30].…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical assaying of DNA methyltransferase activity based on mimic-hybridization chain reaction amplified strategy

Zhang

Liu

et al. 2016

Analytica Chimica Acta

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G-quadruplex functionalized nano mesoporous silica for assay of the DNA methyltransferase activity

Cited by 20 publications

References 48 publications

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Microfluidic platforms for DNA methylation analysis

Sensitive electrochemical assaying of DNA methyltransferase activity based on mimic-hybridization chain reaction amplified strategy

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