G-quadruplex specific thioflavin T-based label-free fluorescence aptasensor for rapid detection of tetracycline

Dai, Yuanyuan; Zhang, Ying; Liao, Wanliang; Wang, Weiping; Wu, Liang

doi:10.1016/j.saa.2020.118406

Cited by 23 publications

(10 citation statements)

References 45 publications

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“…The content of G and C bases in Apt76 is as high as 68.4%, which provides a foundation for the synthesis of AgNCs. In addition, it has been reported that Apt76 in buffer can form a G-quadruplex structure [30],…”

Section: Principle Of the Label-free Biosensor Based On Agncsmentioning

confidence: 99%

A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline

Yang

Zhan

et al. 2023

J Nanobiotechnol

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Tetracycline (TET) is a broad-spectrum antibiotic commonly used in the treatment of animals. TET residues in food inevitably threaten human health. High-performance analytical techniques for TET detection are required in food quality assessment. The objective of this study was to establish a label-free fluorescent biosensor for TET detection using specific aptamer-templated silver nanoclusters (AgNCs). An aptamer with a high specific binding ability to TET was used to synthesize a novel DNA-templated AgNCs (DNA-AgNCs). When TET is present, the aptamer’s conformation switched from an antiparallel G-quadruplex to a hairpin structure, altering the connection between AgNCs and the aptamer. Following the transformation of AgNCs into large sized silver nanoparticles (AgNPs), a fluorescence decrease was detected. When used to detect TET in milk, the proposed biosensor displayed high sensitivity and selectivity, with a limit of detection of 11.46 ng/mL, a linear range of 20 ng/mL−10 g/mL, and good recoveries of 97.7–114.6% under optimized conditions. These results demonstrate that the proposed biosensor was successfully used to determine TET quantitatively in food samples, suggesting that our method provides an efficient and novel reference for detecting antibiotics in food while expanding the application of DNA-AgNCs in related fields.

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Section: Principle Of the Label-free Biosensor Based On Agncsmentioning

confidence: 99%

A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline

Yang

Zhan

et al. 2023

J Nanobiotechnol

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“…S4 †), the rate and mutation abundance showed a linear relation in the range of 0-100%. Compared with other detection methods, [12][13][14][15][16] this method has a lower mutation detection limit and is shorter in time. All the substances used in the Exo III-amplied 4-way strand migration system are relatively low-cost.…”

Section: Detection Limit Of Low Abundance Deletion Mutations In Synth...mentioning

confidence: 99%

“…In order to acquire high sensitivity for EGFR exon 19 deletions, a more simple and efficient design model that combined PCR with rolling circle amplication, graphene oxide, locked nucleic acid and more was researched. [12][13][14] Unfortunately, the cost of the material was relatively expensive, and the sensitivity was limited by PCR itself (the detection limit is about 0.1%). 15,16 The nucleic acid probe methods have the advantages of strong adjustment, simple operation, and rapid detection.…”

Section: Introductionmentioning

confidence: 99%

An exonuclease III-amplified 4-way strand migration system for low-abundance deletion mutation

Tang

Wang

et al. 2022

Anal. Methods

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“…Based on the G4 structure of the TET aptamer, Dai et al developed a label-free aptasensor for TET with ThT as a fluorescent probe . Owing to the aptamer-TET interaction and concomitant disassembly of the G4 structure, the fluorescence intensity gradually decreased with increasing TET concentration, resulting in an LOD of 1 nM (∼0.4 ng mL –1 ).…”

Section: Application Of Fluorescent Aptasensor For the Detection Of F...mentioning

confidence: 99%

“…Based on the G4 structure of the TET aptamer, Dai et al developed a label-free aptasensor for TET with ThT as a fluorescent probe. 165 Owing to the aptamer-TET interaction and concomitant disassembly of the G4 structure, the fluorescence intensity gradually decreased with increasing TET concentration, resulting in an LOD of 1 nM (∼0.4 ng mL −1 ). Relying on the interaction between NM quencher and aptamer-functionalized fluorescent nanoprobes, turn-on fluorescence aptasensors were constructed to detect TET.…”

Section: ■ Application Of Fluorescent Aptasensor For the Detection Of...mentioning

confidence: 99%

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

et al. 2021

Anal. Chem.

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G-quadruplex specific thioflavin T-based label-free fluorescence aptasensor for rapid detection of tetracycline

Cited by 23 publications

References 45 publications

A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline

A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline

An exonuclease III-amplified 4-way strand migration system for low-abundance deletion mutation

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

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