G1 cyclins block the Ime1 pathway to make mitosis and meiosis incompatible in budding yeast

Colomina, Neus; Garí, Eloi; Gallego, Carme; Herrero, Enrique; Aldea, Martí

doi:10.1093/emboj/18.2.320

Cited by 91 publications

(144 citation statements)

References 46 publications

(100 reference statements)

Supporting

Mentioning

136

Contrasting

Order By: Relevance

“…In addition to these classical regulators of IME1, other regulatory factors include the respiration potential of the cell, the storage carbohydrate trehalose, the G1 cyclins, and extracellular pH (Colomina et al 1999;De SilvaUdawatta and Cannon 2001;Jambhekar and Amon 2008). Trehalose promotes Ime1 expression, possibly via the kinase Mck1, while G1 cyclins repress its expression (Colomina et al 1999;De Silva-Udawatta and Cannon 2001).…”

Section: Entry Into Sporulation: Control Of Ime1 Activitymentioning

confidence: 99%

See 1 more Smart Citation

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

View full text Add to dashboard Cite

In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

show abstract

Section: Entry Into Sporulation: Control Of Ime1 Activitymentioning

confidence: 99%

“…Trehalose promotes Ime1 expression, possibly via the kinase Mck1, while G1 cyclins repress its expression (Colomina et al 1999;De Silva-Udawatta and Cannon 2001). This latter control may help ensure that cells enter the sporulation pathway from early in G1, before G1 cyclins accumulate.…”

Section: Entry Into Sporulation: Control Of Ime1 Activitymentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

View full text Add to dashboard Cite

show abstract

“…In budding yeast, nutrient deprivation causes G1 cell cycle arrest and meiotic initiation (Honigberg and Purnapatre, 2003;Wittenberg and La Valle, 2003). Cell cycle regulators appear to play an important role in this switch, keeping the mitotic cell cycle and meiotic initiation mutually exclusive (Colomina et al, 1999). In animals, nutrient deprivation is not the signal for meiotic entry.…”

Section: Introductionmentioning

confidence: 99%

Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline

et al. 2011

View full text Add to dashboard Cite

SUMMARYThe C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cycling cells and cells in meiotic S phase, followed by entry into meiotic prophase. The proliferative fate is specified by somatic distal tip cell (DTC) niche-germline GLP-1 Notch signaling through repression of the redundant GLD-1 and GLD-2 pathways that promote entry into meiosis. Here, we describe characteristics of the proliferative zone, including cell cycle kinetics and population dynamics, as well as the role of specific cell cycle factors in both cell cycle progression and the decision between the proliferative and meiotic cell fate. Mitotic cell cycle progression occurs rapidly, continuously, with little or no time spent in G1, and with cyclin E (CYE-1) levels and activity high throughout the cell cycle. In addition to driving mitotic cell cycle progression, CYE-1 and CDK-2 also play an important role in proliferative fate specification. Genetic analysis indicates that CYE-1/CDK-2 promotes the proliferative fate downstream or in parallel to the GLD-1 and GLD-2 pathways, and is important under conditions of reduced GLP-1 signaling, possibly corresponding to mitotically cycling proliferative zone cells that are displaced from the DTC niche. Furthermore, we find that GLP-1 signaling regulates a third pathway, in addition to the GLD-1 and GLD-2 pathways and also independent of CYE-1/CDK-2, to promote the proliferative fate/inhibit meiotic entry.

show abstract

“…Mechanisms driving entry into pre-mitotic and pre-meiotic S-phases might be different as conditions that trigger entry into the two phases are contrastingly different (presence vs. absence of nutrients). In addition, the Cln-Cdk's inhibit IME1 expression making entries into mitosis and meiosis mutually exclusive [18]. Cln3-Cdk induces expression of CLN1 and CLN2 during pre-mitotic G1 phase.…”

Section: G1-s Transitionmentioning

confidence: 99%