GABA-like immunoreactive neurons in the retina of Bufo marinus: evidence for the presence of GABA-containing ganglion cells

Gábriel, Róbert; Straznicky, Charles; Wye-Dvorak, Judy

doi:10.1016/0006-8993(92)90528-h

Cited by 39 publications

(21 citation statements)

References 24 publications

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“…More than half of the amacrine cells (Gá briel et al, 1992a;Mosinger et al, 1986) are GABA-positive and consequently, more than 50% of the synapses in the frog IPL contain at least one participant element containing GABA. GABAergic synapses could be identified in all five sublaminas of the IPL, though the distribution of the synapse types differed.…”

Section: Synapses Of Gabaergic Elementsmentioning

confidence: 99%

Synaptology of the inner plexiform layer in the anuran retina

Gábriel

Rabl

Veisenberger

2000

Microsc. Res. Tech.

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Section: Synapses Of Gabaergic Elementsmentioning

confidence: 99%

Synaptology of the inner plexiform layer in the anuran retina

Gábriel

Rabl

Veisenberger

2000

Microsc. Res. Tech.

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“…Amacrine cells and DAs represent heterogenous groups both in terms of their morphology and/or cytochemical 1990bGfibriel et al 1992 markers. In the retina of Bufo marinus we have described substance P- (Hiscock and Straznicky 1989a), neuropeptide Y- (Hiscock and Straznicky 1989 b), serotonin- (Zhu and Straznicky 1990a), dopamine- (Zhu and Straznicky 1990 b) and GABA-containing (Gfibriel et al 1992) ACs.…”

Section: The Nature Of Displaced Amacrine Ceilsmentioning

confidence: 99%

“…In the retina of Bufo marinus we have described substance P- (Hiscock and Straznicky 1989a), neuropeptide Y- (Hiscock and Straznicky 1989 b), serotonin- (Zhu and Straznicky 1990a), dopamine- (Zhu and Straznicky 1990 b) and GABA-containing (Gfibriel et al 1992) ACs. Each type is represented in the GCL as DAs (Table 3).…”

Section: The Nature Of Displaced Amacrine Ceilsmentioning

confidence: 99%

The generation and changing retinal distribution of displaced amacrine cells in Bufo marinus from metamorphosis to adult

Chng

Straznicky

1992

Anat Embryol

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The generation and retinal distribution of displaced amacrine cells (DAs) were studied from metamorphosis to adult in the cane toad Bufo marinus. Displaced amacrine cells were identified by inducing chromatolytic changes in ganglion cells (GCs) following optic nerve section. Cells that did not chromatolyse in the ganglion cell layer (GCL) of the retina were regarded as DAs. The number of DAs increased considerably from an estimated 10,000 at metamorphosis to 211,000 in the adult toad, and was accompanied by a substantial decrease of average cell density. In contrast to the reported 6:1 cell density gradient of all cells of the GCL in adult toad (Nguyen and Straznicky 1989) only a shallow 1.6:1 density gradient of DAs from the visual streak to the dorsal and ventral retinal margins was detected. Consequently, the incidence of DAs increased from 15% of all cells of the GCL in the visual streak to 30% in the dorsal and ventral peripheral retina. These results indicate that the ratio of the newly generated DAs and GCs at the ciliary margin must be changing during development. More GCs are generated before and around metamorphosis then DAs, in contrast to the relative increase of the percentage of DAs generated after metamorphosis. The possible control of the numbers of DAs in the GCL is discussed.

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“…First, [ 3 H] GABA uptake was observed in bipolar cells in Rana frog (Voaden et al, 1974) and mudpuppy (Pourcho et al, 1984). Subsequently, a small population of bipolar cells were found to be immunoreactive (IR) for glutamate decarboxylase (GAD) in mudpuppy (Yazulla, 1986) and Rana frog (Agardh, 1987a;Tyler et al, 1995), and GABA-IR was found in bipolar cells in mudpuppy (Mosinger et al, 1986), Bufo toad (Gá briel et al, 1992), Rana frog (Mosinger et al, 1986;Osborne et al, 1986;Agardh et al, 1987a), and Xenopus frog (Zhu and Straznicky, 1993). Recently, we have obtained strong evidence in the salamander retina demonstrating that a small but significant population of orthotopic type I and type II bipolar cells may be GABAergic.…”

mentioning

confidence: 94%

L-Glutamic acid decarboxylase- and ?-aminobutyric acid-immunoreactive bipolar cells in tiger salamander retina are of ON- and OFF-response types as inferred from Lucifer Yellow injection

Yang

1997

J. Comp. Neurol.

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The bipolar cells in vertebrate retinas are considered to be excitatory in nature and use L-glutamate as their neurotransmitter. Our earlier studies have provided evidence demonstrating that a small but significant population of orthotopic bipolar cells in salamander retina may be gamma-aminobutyric acid (GABA)ergic. In this work, the stratification levels of axon terminals in the inner plexiform layer (IPL) of single L-glutamic acid decarboxylase-immunoreactive (GAD-IR) and GABA-immunoreactive (GABA-IR) bipolar cells in the salamander retinal slices were studied. GAD-IR and GABA-IR bipolar cells marked by a fluorescent probe, Texas Red, were injected with Lucifer Yellow (LY) through a patch pipette under visual control. A total number of 42 GAD-IR bipolar cells in 24 slices and 84 GABA-IR bipolar cells in 56 slices were injected. Among these, terminals of nine GAD-IR bipolar cells and 22 GABA-IR bipolar cells were sufficiently filled with LY for determination of the stratification levels in the IPL. The stratification patterns and levels of GAD-IR and GABA-IR bipolar cells were very similar. GAD-IR and GABA-IR orthotopic type I and type II bipolar cells (soma located in the most distal or middle of the inner nuclear layer [INL], respectively), had their axon terminals stratified in sublamina a and sublamina b of the IPL with comparable frequency. Axonal processes were restricted largely to either the distal or the proximal region within sublaminae a and b. In addition, three of the bipolar cells had their terminals located in the middle region of the IPL. The similarities of stratification patterns and levels between GAD-IR and GABA-IR type I and type II bipolar cells indicate that they represent the same population of presumed GABAergic bipolar cells. Based on comparative stratifications of GABA bipolar cells reported here and those derived from electrophysiological studies (Hensley et al. [1993] J. Neurophysiol. 69:2086-2098), it is suggested that putative GABAergic bipolar cells represent cone-dominated and rod-dominated ON- and OFF-bipolar cells and that they subserve a broad role in the ON- and OFF-visual pathways in the retina.

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GABA-like immunoreactive neurons in the retina of Bufo marinus: evidence for the presence of GABA-containing ganglion cells

Cited by 39 publications

References 24 publications

Synaptology of the inner plexiform layer in the anuran retina

Synaptology of the inner plexiform layer in the anuran retina

The generation and changing retinal distribution of displaced amacrine cells in Bufo marinus from metamorphosis to adult

L-Glutamic acid decarboxylase- and ?-aminobutyric acid-immunoreactive bipolar cells in tiger salamander retina are of ON- and OFF-response types as inferred from Lucifer Yellow injection

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