GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Hiura, Akio; Ishizuka, Hiroshi; Villalobos, Elizabeth López

doi:10.1679/aohc.54.195

Cited by 25 publications

(11 citation statements)

References 22 publications

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“…Previous studies of GABA-immunoreactivity have identified local interneurons as one possible source of cells for this immunoreactivity in spinal cord (Basbaum et al, 1986;Hiura et al, 1991;Powell and Todd, 1992;Todd and McKenzie, 1989). Light microscopic immunolabeling studies have shown GABAergic neurons in superficial PC/MDH (Ginestal and Matute, 1993;Todd et al, 1992a).…”

Section: ) This Class Of R Vesicle-containing Terminal Inmentioning

confidence: 97%

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Almond

Westrum

Henry

1996

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This study examines the normal synaptic organization of the feline spinal trigeminal nucleus pars caudalis (PC). A primary goal of this study is to identify and characterize the synaptic complexes within PC based on their specific neurotransmitter content. Post-embedding immunogold techniques are utilized with electron microscopy to determine the ultrastructural localization of gamma-aminobutyric acid (GABA) immunoreactivity within lamina II of PC. The colloidal gold particles (10 nm) are randomly distributed over immunoreactive (IR) profiles without preference toward membranous or cytoplasmic regions. GABA immunoreactivity occurs on small unmyelinated axons, on terminals which form synaptic contacts, and on some vesicle-containing dendrites. The GABA-IR terminals form symmetric (type II) contracts onto unlabeled somata and dendrites of various sizes, and onto other unlabeled axon terminals. The GABA-IR terminal in axo-axonic complexes is presynaptic to a round vesicle-containing terminal, which itself may form a type I asymmetric contact onto an unlabeled dendrite or soma. A proportion of vesicle-containing dendrites show GABA-immunoreactivity and are post-synaptic to unlabeled terminals with round vesicles. Other, but far fewer, vesicle-containing dendrites are GABA negative and postsynaptic to GABA-IR terminals. In summary, the findings are consistent with the localization of GABA in intrinsic neurons, and may be associated with presynaptic and postsynaptic inhibition within nociceptive related pathways.

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Section: ) This Class Of R Vesicle-containing Terminal Inmentioning

confidence: 97%

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Almond

Westrum

Henry

1996

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“…Barber et al (1982) demonstrated that some of the ependymal cells of the rat spinal cord contain GABA. More recently, Hiura et al (1991) identified GABA-positive synaptic vesicles terminating on blood capillaries and suggested that GABA may be released in blood circulation. The detection of GABA and of the GAD isoforms in non-neural cells Petersen et al, 1993) as well as their involvement in diabetes pathology (Honeyman et al, 1993;Michelsen et al, 1993) reinforce the concept that GABA could be a more general signaling molecule in addition to being a neurotransmitter.…”

Section: Functional Significance Of the Different Pattern Of Gad Labementioning

confidence: 99%

Comparative distribution of GAD₆₅ and GAD₆₇ mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo

Feldblum

Dumoulin

Anoal

et al. 1995

J of Neuroscience Research

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Gamma-aminobutyric acid (GABA) synthesis can result from the action of at least two glutamic acid decarboxylase (GAD) isoforms, GAD65 and GAD67, possibly involved in distinct mechanisms. We have made the hypothesis that GAD65 may respond to short-term changes and is present in neurons with a phasic activity, while GAD67 may rather provide GABA for the metabolic pool and for supporting tonic levels of synaptic transmission (Erlander et al.: Neuron 7:91-100, 1991; Feldblum et al.: J Neurosci Res 34:689-706, 1993). In the present work we have tested this hypothesis in the rat spinal cord where both types of activities have been identified. The correlation of GABA immunodetection with the distribution of GAD65 and GAD67 mRNAs and proteins has evinced in the dorsal horn a differential regulation of the two isoforms. In situ hybridization has revealed, in the dorsal horn, relatively higher levels of GAD67 mRNA than of GAD65, while immunodetection of the proteins demonstrated numerous punctate profiles with both GAD antisera. Reverse transcription-polymerase chain reaction (RT-PCR) data confirmed the abundance of the GAD67 transcripts compared to GAD65 in the rat spinal cord. In contrast, within the ventral horn, there was a greter number of GAD67-immunoreactive (IR) profiles mostly located around motoneurons. The paucity of GAD65 immunoreactivity in the ventral horn cannot be related to a different accessibility of the antigens to the epitopes since on the same section a dense GAD65 staining was detected in the dorsal horn. Hence, a number of biochemical and electrophysiological data support the concept of the involvement of glycine as the major inhibitory system within the ventral horn which may explain the low levels of GAD transcription in this region. The paucity of GAD65 in the ventral horn may also reflect a functional difference, suggesting a predominance of GAD67 in neurons under tonic activity. In the dorsal horn, where neurons with phasic and tonic firing patterns have been disclosed, GAD65 may, in addition, provide GABA for responses to short-term changes.

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“…During development, they generate the dark, large scalloped central glomerular endings [21]. The same type of CI terminals were demonstrated in the mouse substantia gelatinosa [6,9]. Besides the typical, dark, large scalloped CI terminals, small round, slender or dark sinuous terminals with closely packed or less packed clear synaptic vesicles were easily visualized, even in the adult mouse substantia gelatinosa.…”

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confidence: 57%

Fluoride-Resistant Acid Phosphatase (FRAP)-Positive Primary Afferent Central Terminals in the Mouse Substantia Gelatinosa.

Hiura

Ishizuka

Ohta

1995

Acta Histochem. Cytochem

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GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Cited by 25 publications

References 22 publications

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Comparative distribution of GAD₆₅ and GAD₆₇ mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo

Fluoride-Resistant Acid Phosphatase (FRAP)-Positive Primary Afferent Central Terminals in the Mouse Substantia Gelatinosa.

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GABAergic neurons in the mouse superficial dorsal horn with special emphasis on their relation to primary afferent central terminals.

Cited by 25 publications

References 22 publications

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Post-embedding immunogold labeling of gamma-aminobutyric acid in lamina II of the spinal trigeminal subnucleus pars caudalis: I. A qualitative study

Comparative distribution of GAD65 and GAD67 mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo

Fluoride-Resistant Acid Phosphatase (FRAP)-Positive Primary Afferent Central Terminals in the Mouse Substantia Gelatinosa.

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Comparative distribution of GAD₆₅ and GAD₆₇ mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo