Galactosylated chitosan/DNA nanoparticles prepared using water-soluble chitosan as a gene carrier

Kim, Tae Hee; Park, In Kyu; Nah, Jae Woon; Choi, Yun Jaie; Cho, Chong Su

doi:10.1016/j.biomaterials.2003.10.063

Cited by 250 publications

(173 citation statements)

References 43 publications

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“…Similarly, LA bearing galactose group was coupled with water soluble chitosan (WSC) for hepatocyte specifi city. The transfection effi ciency into HepG2, which possesses ASGP-R, was higher than that recorded in case of HeLa without ASGP-R (Kim et al 2004).…”

Section: Chitosanmentioning

confidence: 65%

Nano-vectors for efficient liver specific gene transfer

Pathak

Vyas²,

Gupta³

2008

IJN

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Recent progress in nanotechnology has triggered the site specifi c drug/gene delivery research and gained wide acknowledgment in contemporary DNA therapeutics. Amongst various organs, liver plays a crucial role in various body functions and in addition, the site is a primary location of metastatic tumor growth. In past few years, a plethora of nano-vectors have been developed and investigated to target liver associated cells through receptor mediated endocytosis. This emerging paradigm in cellular drug/gene delivery provides promising approach to eradicate genetic as well as acquired diseases affecting the liver. The present review provides a comprehensive overview of potential of various delivery systems, viz., lipoplexes, liposomes, polyplexes, nanoparticles and so forth to selectively relocate foreign therapeutic DNA into liver specifi c cell type via the receptor mediated endocytosis. Various receptors like asialoglycoprotein receptors (ASGP-R) provide unique opportunity to target liver parenchymal cells. The results obtained so far reveal tremendous promise and offer enormous options to develop novel DNAbased pharmaceuticals for liver disorders in near future.

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Section: Chitosanmentioning

confidence: 65%

Nano-vectors for efficient liver specific gene transfer

Pathak

Vyas²,

Gupta³

2008

IJN

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“…[29][30][31][32][33], poly-glutamic acid (PLGA) [34][35][36], polyethlenimine (PEI) [37][38][39][40][41][42], chitosan [43][44][45][46][47], dendrimer/α-cyclodextrin conjugate [48], and their derivatives [49,50] have been studied for galactosylated macromolecular carriers for hepatocyte-selective targeting. The design of carrier types based on the physicochemical properties and galactose density of the carriers are of significance for targeting efficacy by galactosylated macromolecular carriers.…”

Section: Overcoming the Rapid Clearance Of Macromolecular Carriersmentioning

confidence: 99%

Glycosylation-mediated targeting of carriers

Kawakami

Hashida

2014

Journal of Controlled Release

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For safe and effective therapy, drugs should be delivered selectively to their target tissues or cells at an optimal rate. Drug delivery system technology maximizes the therapeutic efficacy and minimizes unfavorable drug actions by controlling their distribution profiles. Ligand-receptor binding is a typical example of specific recognition mechanisms in the body; therefore, ligand-modified drug carriers have been developed for active targeting based on receptor-mediated endocytosis. Among the various ligands reported thus far, sugar recognition is a promising approach for active targeting because of their high affinity and expression. Glycosylation has been applied for both macromolecular and liposomal carriers for cell-selective drug targeting.Recently, the combination of ultrasound exposure and glycosylated bubble liposomes has been developed. In this review, recent advances of glycosylation-mediated targeted drug delivery systems are discussed.

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“…4,5 However, this system is significantly limited by the low transfection efficiency and low cell specificity of chitosans. 6 To address these limitations, several ligands, such as chitosan modified by folate, 6,7 transferrin, 8 mannose, 9,10 and galactose, 11,12 have been designed and evaluated for receptormediated endocytotic gene delivery. Of these, folate chitosans have been reported as cancer cell-targeting gene carriers because of specific ligand-receptor interactions between folate moieties and the folate receptor, which are absent in most normal tissues at a high frequency, but are amplified in a variety of human cancers, including liver cancer.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic efficiency of folated poly(ethylene glycol)-chitosan-graft-polyethylenimine-Pdcd4 complexes in H-ras12V mice with liver cancer

Kim

Minai-Tehrani²,

Lee³

et al. 2013

IJN

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Background: Chitosan and chitosan derivatives have been proposed as alternative and biocompatible cationic polymers for nonviral gene delivery. However, the low transfection efficiency and low specificity of chitosan is an aspect of this approach that must be addressed prior to any clinical application. In the present study, folated poly(ethylene glycol)-chitosan-graftpolyethylenimine (FPCP) was investigated as a potential folate receptor-overexpressed cancer cell targeting gene carrier. Methods: The FPCP copolymer was synthesized in two steps. In the first step, folate-PEG was synthesized by an amide formation reaction between the activated carboxyl groups of folic acid and the amine groups of bifunctional poly(ethylene glycol) (PEG). In the second step, FPCP was synthesized by an amide formation reaction between the activated carboxyl groups of folate-PEG and amine groups of CHI-g-polyethyleneimine (PEI). The composition of FPCP was characterized by 1 H nuclear magnetic resonance. Results: FPCP showed low cytotoxicity in various cell lines, and FPCP-DNA complexes showed good cancer cell specificity as well as good transfection efficiency in the presence of serum. Further, FPCP-Pdcd4 complexes reduced tumor numbers and progression more effectively than PEI 25 kDa in H-ras12V liver cancer mice after intravenous administration. Conclusion: Our data suggest that FPCP, which has improved transfection efficiency and cancer cell specificity, may be useful in gene therapy for liver cancer.

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Galactosylated chitosan/DNA nanoparticles prepared using water-soluble chitosan as a gene carrier

Cited by 250 publications

References 43 publications

Nano-vectors for efficient liver specific gene transfer

Nano-vectors for efficient liver specific gene transfer

Glycosylation-mediated targeting of carriers

Therapeutic efficiency of folated poly(ethylene glycol)-chitosan-graft-polyethylenimine-Pdcd4 complexes in H-ras12V mice with liver cancer

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