Galanin promotes autophagy and alleviates apoptosis in the hypertrophied heart through FoxO1 pathway

Martinelli, Ilenia; Timotin, Andreï; Moreno-Corchado, Paula; Marsal, Dimitri; Kramar, S. B.; Loy, Halina; Joffre, Carine; Boal, Frédéric; Tronchère, Hélène; Kunduzova, Oksana

doi:10.1016/j.redox.2021.101866

Cited by 26 publications

(26 citation statements)

References 46 publications

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“…However, nonneural expression of GAL-like immunoreactivity has been reported in several other tissues and cells: fibroblast cell lines (Yamamoto et al 2014); primary epidermal keratinocytes (Lee et al 2017); macrophage-like cells of the synovial membrane (Wu et al 2004); fibroblast-like cells in skin during the formation of granulation tissue (Yamamoto et al 2011); osteoprogenitor cells, osteoblasts, and chondrocytes of the hypertrophic zone during rib fracture repair (McDonald et al 2003); and blood vessels, hair follicles, and sweat glands (Kofler et al 2004; Lang et al 2007). Moreover, previous studies suggested that GAL is involved in the regulation of inflammatory processes (Koller et al 2017; Koller et al 2019; Brzozowska and Calka 2021; Martinelli et al 2021). Earlier studies have speculated about an application of GAL in tissue regeneration (McDonald et al 2003; Yamamoto et al 2011; Yamamoto et al 2014), and one study applied GAL injections to promote calvarial bone formation via an injection strategy (McDonald et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Positive effects of GAL on bone formation and remodeling have also been reported in the mouse calvarial defect model mentioned earlier (McDonald et al 2007). While the mechanism by which GAL regulates tissue regeneration is currently unknown, it has been shown that GAL affects cardiomyoblasts through the FoxO1 pathway (Martinelli et al 2021), a possible candidate pathway as FoxO family members have been implicated in the regulation of osteoprogenitors, osteoblasts, and osteoclasts (Alharbi et al 2018; Ma et al 2020). In addition, anti-inflammatory effects of GAL have been indicated in animal models with chronic inflammatory diseases (Talero et al 2006; Talero et al 2007; Brzozowska and Calka 2021).…”

Section: Discussionmentioning

confidence: 99%

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Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration

Lyu

Pandya

et al. 2021

J Dent Res

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The nervous system exerts finely tuned control over all aspects of the life of an organism, including pain, sensation, growth, and development. Recent developments in tissue regeneration research have increasingly turned to small molecule peptides to tailor and augment the biological response following tissue loss or injury. In the present study, we have introduced the small molecule peptide galanin (GAL) as a novel scaffold-coating agent for the healing and regeneration of craniofacial tissues. Using immunohistochemistry, we detected GAL and GAL receptors in healthy periodontal tissues and in the proximity of blood vessels, while exposure to our periodontal disease regimen resulted in a downregulation of GAL. In a 3-dimensional bioreactor culture, GAL coating of collagen scaffolds promoted cell proliferation and matrix synthesis. Following subcutaneous implantation, GAL-coated scaffolds were associated with mineralized bone-like tissue deposits, which reacted positively for alizarin red and von Kossa, and demonstrated increased expression and protein levels of RUNX2, OCN, OSX, and iBSP. In contrast, the GAL receptor antagonist galantide blocked the effect of GAL on Runx2 expression and inhibited mineralization in our subcutaneous implantation model. Moreover, GAL coating promoted periodontal regeneration and a rescue of the periodontal defect generated in our periodontitis model mice. Together, these data demonstrate the efficacy of the neuropeptide GAL as a coating material for tissue regeneration. They are also suggestive of a novel role for neurogenic signaling pathways in craniofacial and periodontal regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration

Lyu

Pandya

et al. 2021

J Dent Res

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“…Galanin is expressed mainly in the nervous system and in some peripheral organs, but no expression in cardiomyocytes has been reported (Palkeeva et al, 2019). However, its receptors are expressed in various cell types, including cardiomyocytes, and it has been suggested to be cardioprotective (Fang et al, 2013; Studneva et al, 2020; Serebryakova et al, 2019; Palkeeva et al, 2019; Martinelli et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…However, its receptors are expressed in various cell types, including cardiomyocytes, and it has been suggested to be cardioprotective (Fang et al, 2013;Studneva et al, 2020;Serebryakova et al, 2019;Palkeeva et al, 2019;Martinelli et al, 2021).…”

Section: Several Genes Associated With Both Apoptosis and Cardiomyocyte Hypertrophy Such As Cryab Eno1mentioning

confidence: 99%

Transcriptomics reveal stretched human pluripotent stem cell-derived cardiomyocytes as an advantageous hypertrophy model

Pohjolainen

Ruskoaho

Talman

2021

Preprint

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Left ventricular hypertrophy, characterized by hypertrophy of individual cardiomyocytes, is an adaptive response to an increased cardiac workload that eventually leads to heart failure. Previous studies using neonatal rat ventricular myocytes (NRVMs) and animal models have revealed several hypertrophy- and mechanical load-associated genes and signaling pathways. However, these models are not directly applicable to humans. Here, we studied the effect of cyclic mechanical stretch on gene expression of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) using RNA sequencing. HiPSC-CMs showed distinct hypertrophic changes in gene expression at the level of individual genes and in biological processes. We also identified several differentially expressed genes that have not been previously associated with cardiomyocyte hypertrophy and thus serve as attractive targets for future studies. When compared to previously published data attained from stretched NRVMs and human embryonic stem cell-derived cardiomyocytes, hiPSC-CMs displayed a smaller number of changes in gene expression, but the differentially expressed genes revealed more pronounced enrichment of hypertrophy-related biological processes and pathways. Overall, these results establish hiPSC-CMs as a valuable in vitro model for studying human cardiomyocyte hypertrophy.

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“…A mouse model of I/R was used as previously described [43]. The mice were intubated and placed under mechanical ventilation after undergoing general anesthesia with an intraperitoneal injection of ketamine (125 mg/kg) and xylazine (10 mg/kg).…”

Section: Experimental Protocolmentioning

confidence: 99%

Metformin Attenuates Postinfarction Myocardial Fibrosis and Inflammation in Mice

Loi

Kramar

Laborde

et al. 2021

IJMS

Self Cite

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Diabetes is a major risk factor for the development of cardiovascular disease with a higher incidence of myocardial infarction. This study explores the role of metformin, a first-line antihyperglycemic agent, in postinfarction fibrotic and inflammatory remodeling in mice. Three-month-old C57BI/6J mice were submitted to 30 min cardiac ischemia followed by reperfusion for 14 days. Intraperitoneal treatment with metformin (5 mg/kg) was initiated 15 min after the onset of reperfusion and maintained for 14 days. Real-time PCR was used to determine the levels of COL3A1, αSMA, CD68, TNF-α and IL-6. Increased collagen deposition and infiltration of macrophages in heart tissues are associated with upregulation of the inflammation-associated genes in mice after 14 days of reperfusion. Metformin treatment markedly reduced postinfarction fibrotic remodeling and CD68-positive cell population in mice. Moreover, metformin resulted in reduced expression of COL3A1, αSMA and CD68 after 14 days of reperfusion. Taken together, these results open new perspectives for the use of metformin as a drug that counteracts adverse myocardial fibroticand inflammatory remodeling after MI.

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Galanin promotes autophagy and alleviates apoptosis in the hypertrophied heart through FoxO1 pathway

Cited by 26 publications

References 46 publications

Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration

Successful Application of a Galanin-Coated Scaffold for Periodontal Regeneration

Transcriptomics reveal stretched human pluripotent stem cell-derived cardiomyocytes as an advantageous hypertrophy model

Metformin Attenuates Postinfarction Myocardial Fibrosis and Inflammation in Mice

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