Galectin‐3 does not reliably distinguish benign from malignant thyroid neoplasms

Mehrotra, Pallavi; Okpokam, Atuora; BouHaidar, Ralph; Johnson, Sarah J.; Wilson, Janet A.; Davies, Barry R.; Lennard, T. W. J.

doi:10.1111/j.1365-2559.2004.01978.x

Cited by 102 publications

(80 citation statements)

References 28 publications

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“…Although many reports have been published on this issue during the last decade (about 100 papers are displayed in Pub Med since 1997) some authors experienced conflicting results with the use of galectin-3 for improving diagnosis of follicular thyroid proliferations (Martins et al, 2002;Niedziela et al, 2002;Feilchenfeldt et al, 2003;JakubiakWielganowicz et al, 2003;De-Leon-Mazariegos et al, 2004;Mehrotra et al, 2004;Mills et al, 2005). However, some of these reports show major methodological problems (Herrmann et al, 2002;Bartolazzi et al, 2003;Bartolazzi and Bussolati, 2006).…”

Section: Discussionmentioning

confidence: 99%

Large needle aspiration biopsy and galectin-3 determination in selected thyroid nodules with indeterminate FNA-cytology

et al. 2006

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Thyroid fine-needle aspiration biopsy (FNA)-cytology is widely used for the preoperative characterisation of thyroid nodules but this task is difficult for follicular lesions, which often remain undefined. We propose a strategy for improving the preoperative characterisation of selected follicular thyroid proliferations, which is based on large needle aspiration biopsy (LNAB) and galectin-3 expression analysis. Eighty-five thyroid specimens were obtained by LNAB (20-gauge needles) from thyroid nodules with indeterminate follicular FNA-cytology. Aspirated material was processed as a tissue microbiopsy to obtain cell blocks for both cyto/ histo-morphological evaluation and galectin-3 expression analysis, by using a purified monoclonal antibody to galectin-3 and a biotinfree immunoperoxidase staining method. Preoperative diagnosis was compared to the final histology. LNAB and cell-block technique allow a preliminary distinction between nodules with a homogeneous microfollicular/trabecular structure, as frequently observed in tumours, and lesions with mixed normo -micro -macrofollicular architecture, as observed in goitre. Furthermore, LNAB provides optimal substrates for galectin-3 expression analysis. Among 85 cases tested, 14 galectin-3-positive cases were discovered preoperatively (11 thyroid cancers and three adenomas confirmed at the final histology), whereas galectin-3-negative cases were 71 (one carcinoma and 70 benign proliferations at the final histology). Sensitivity, specificity and diagnostic accuracy of this integrated morphologic and phenotypic diagnostic approach were 91.6, 97.2 and 95.3%, respectively. In conclusion, LNAB plus galectin-3 expression analysis when applied preoperatively to selected thyroid nodules candidate to surgery can potentially reduce unnecessary thyroid resections.

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Section: Discussionmentioning

confidence: 99%

Large needle aspiration biopsy and galectin-3 determination in selected thyroid nodules with indeterminate FNA-cytology

et al. 2006

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“…In the present study, the observed specificity of galectin-3 in thyroid carcinomas was 82%. However, some researchers reported conflicting results with the use of the galectin-3-expression test in the diagnosis of follicular thyroid proliferations (25,26). According to Bartolazzi et al, the controversy may result from methodological issues.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of cytokeratin 19, HBME-1, and galectin-3 immunostaining of cell block from fine-needle aspiration specimens in papillary carcinoma of the thyroid

Haltaş¹,

Bayrak²,

Yenıdünya³

et al. 2013

Turk J Med Sci

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In ARIMA modeling studies, the selection of a best model fit to historical data is directly related to whether residual analysis is performed well. Therefore, diagnostic checks including the independence, normality and homoscedasticity of residuals is the most important stage of ARIMA model building. This study is concerned with testing residuals from ARIMA models for monthly streamflow data from the Ç ekerek Stream watershed. Alternative tests including the Ljung-Box Q statistic, runs test and turning point test for independence analysis of the residuals; Kolmogorov-Smirnov and Anderson-Darling tests for normality of residuals; and Goldfeld-Quandt, Breusch and Pagan and Spearman's rho approaches for the homoscedasticity of residuals were used. The selected parsimony model for each data set among the ARIMA models fulfilled the diagnostic checks, considering the Schwarz Bayesian criterion.

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“…Diffi culties in diff erentiating follicular thyroid lesions have instigated large investigations of potential molecular markers that could reliably distinguish benign from malignant thyroid nodules 22,23 . Several diagnostic thyroid markers have been thus evaluated but despite the advantages they possess, most also have certain limitations.…”

Section: Discussionmentioning

confidence: 99%

Potential Diagnostic Markers in Nodular Lesions of the Thyroid Gland: An Immunohistochemical Study

Rydlová¹,

Ludvíková²,

Stankova³

2008

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims:The diff erential diagnosis of thyroid nodules in routine practice can be problemmatic for both pathologists and clinicians. Eff ective treatment requires a determination of the biological nature of the lesions. For this reason, ancilliary diagnostic markers along with histological examination of the nodules may be useful. The objective of this study was to evaluate the diagnostic usefulness of novel markers in the diagnosis of hyperplastic and neoplastic nodules.Methods: Forty eight thyroid lesions forming four diagnostic groups including adenomatous goiters (AS), follicular adenomas (FA), follicular (FC) and papillary carcinomas (PC) were examined using standard immunohistochemical methods. Monoclonal antibodies against galectin-3, matrix metalloproteinases (MMPs) -2 and -7 and endothelial markers CD31 and CD105 were used.Results: The cytoplasmatic expression of galectin-3 was positive in all cases of papillary carcinoma. Moreover, statistically signifi cant diff erences between fused groups of benign (AS and FA) and malignant lesions (FC and PC) were found Fischer's exact test (p = 0.0001). No signifi cant diff erences in cytoplasmic expression of MMPs -2 and -7 and in vascular density assessed by using of both endothelial markers between benign lesions and malignant tumors were revealed.Conclusions: Galectin-3 appears to be a useful marker in the diagnosis of papillary carcinoma only. The matrix metalloproteinases-2 and -7 are not helpful in distinguishing hyperplastic and neoplastic thyroid nodules. Endothelial markers do not appear to be suitable for thyroid diff erential diagnosis. A panel of antibodies in the diff erential diagnosis of thyroid nodular lesions would seem most suitable and further studies with larger sets of patients are awaited.

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Galectin‐3 does not reliably distinguish benign from malignant thyroid neoplasms

Abstract: The data demonstrate that galectin-3 is not a reliable immunohistochemical marker to distinguish benign from malignant thyroid follicular lesions.

Cited by 102 publications

References 28 publications

Large needle aspiration biopsy and galectin-3 determination in selected thyroid nodules with indeterminate FNA-cytology

Large needle aspiration biopsy and galectin-3 determination in selected thyroid nodules with indeterminate FNA-cytology

Diagnostic value of cytokeratin 19, HBME-1, and galectin-3 immunostaining of cell block from fine-needle aspiration specimens in papillary carcinoma of the thyroid

Potential Diagnostic Markers in Nodular Lesions of the Thyroid Gland: An Immunohistochemical Study

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