Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

Silva, Gizele A.L.; Araújo, Luana Brito do Nascimento; Silva, Larissa C.R.; Gouveia, B.B.; Barberino, Ricássio de Sousa; Lins, T.L.B.G.; Monte, Alane Pains Oliveira do; Macedo, T. J. S.; Santos, J.M.S.; Menezes, Vanúzia Gonçalves; Silva, R.L.S.; Matos, Maria Helena Tavares de

doi:10.1016/j.anireprosci.2021.106767

Cited by 5 publications

(3 citation statements)

References 49 publications

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“…In addition, Wang found that miR-29 regulated the proliferation of goat ovarian granulosa cells through the PI3K/AKT/mTOR signalling pathway 43 . Silva's study found that the PI3K pathway acted on ovine ovaries, and gallic acid was used to induce ovine follicle development through PI3K pathway treatment 44 . In conclusion, the upregulation of the COL1A1 gene can activate the PI3K/AKT/mTOR signalling pathway, promote the synthesis of ECM to regulate the proliferation and migration of granulocytes, and affect the development and maturation of goat follicles.…”

Section: Discussionmentioning

confidence: 99%

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

Zhou

Zhao

Chen

et al. 2023

Preprint

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Collagen type I alpha 1 chain (COL1A1), an important component of type I collagen protein, plays a role in regulating follicle development and ovarian cell proliferation, which is closely related to animal reproductive traits. The aim of this study was to explore the relationship between the expression of COL1A1 and lambing traits of Guizhou black goats (Capra hircus), and regulatory mechanisms in follicle development. Results showed that the protein expression level of COL1A1 in ovarian tissues of the polytocous group were higher than those of the monotocous group, and COL1A1 was expressed in all follicles and ovarian cells. Upregulation of COL1A1 significantly promoted cell proliferation, migration and the development of cells from G1 to G2/M phase, reduced intracellular ROS levels. Additionally, COL1A1 overexpression resulted in significantly increased expression of proliferating cell nuclear antigen (PCNA) and the ratios of p-PI3K (phosphorylation-phosphatidylinositol 3-kinase)/PI3K, p-AKT (phosphorylation-protein kinase B)/AKT and p-mTOR (phosphorylation-mammalian target of rapamycin) /mTOR, and decreased expression of BCL2-Associated X (BAX), caspase-3 and caspase-9. The reverse result, however, was seen when COL1A1 expression was downregulated in granulosa cells. Taken together, COL1A1 was widely and highly expressed in ovarian tissues of polytocous goats, and it may participate in regulations of lambing traits by regulating the proliferation and migration of granulosa cells by activating the PI3K/AKT/mTOR signalling pathway and reducing ROS production in granulosa cells to facilitate the development and maturation of follicle.

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Section: Discussionmentioning

confidence: 99%

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

Zhou

Zhao

Chen

et al. 2023

Preprint

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“…Next, the ovaries were transported within 1 h to the laboratory in Corning®tubes (Sarstedt, Newton, NC, USA) containing 0.9% saline solution with antibiotics at 4°C (G.A. Silva et al, 2021a).…”

Section: Source Of Ovarian Tissuementioning

confidence: 99%

“…After in vitro culture, the oocytes were recovered, and intracellular GSH concentrations and mitochondrial activity were determined as previously described (G.A. Silva et al, 2021a). Briefly, oocytes were incubated in the dark for 30 min in phosphatebuffered saline (PBS; pH 7.2) supplemented with 10 mM 4-chloromethyl-6.8-difluoro-7-hydroxycoumarin (CellTracker® Blue; Invitrogen Corporation, Carlsbad, CA, USA) and 100 nM MitoTracker Red (MitoTracker® Red, CMXRos, Molecular Probes, Melbourne, Victoria, Australia) at 39ºC to detect GSH levels as blue fluorescence and mitochondrial activity as red fluorescence.…”

Section: Assessment Of Gsh Concentrations and Mitochondrial Activitymentioning

confidence: 99%

Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Guimarães,

Silva,

Barberino

et al. 2023

Sem. Ci. Agr.

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This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.

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Protective effect of gallic acid on doxorubicin-induced ovarian toxicity in mouse

Silva

Lins

Monte

et al. 2023

Reproductive Toxicology

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Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

Cited by 5 publications

References 49 publications

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles

Protective effect of gallic acid on doxorubicin-induced ovarian toxicity in mouse

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