Gametic Embryogenesis in Triticum: a Study of Some Critical Factors in Haploid (Microspore) Embryogenesis

Sola, Luis Cistué; Kasha, K. J.

doi:10.1007/7089_031

Cited by 7 publications

(11 citation statements)

References 70 publications

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“…In anther and microspore culture, the type of pretreatment and the carbohydrate used in culture medium are factors that affect barley and wheat androgenesis (Cistué and Kasha 2006;Devaux and Kasha 2009;Redha and Talaat 2008). In green plant regeneration, however, osmotic potential and the carbohydrate source also played significant roles in the switch from gametophytic to sporophytic phase of barley and wheat anthers.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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Androgenesis of wheat genotypes was evaluated by pretreating anthers or embryo-like structures (ELS) with polyamines. Anthers of the genotype DH were pretreated with different concentrations of putrescine, spermidine, and spermine for 1, 3, and 6 h, and those of drought-tolerant International Center for Agricultural Research in the Dry Areas (ICARDA) wheat accessions were treated for 1 and 3 h. ELS of two genotypes were also treated for 30 and 60 min with the same polyamines and evaluated for green plant regeneration. The pretreatment of anthers with polyamines enhanced the development of ELS in all genotypes. The formation of ELS varied significantly with genotype. Pretreated anthers showed that four treatments improved significantly green plant regeneration with the genotype ICR 17. However, two treatments (1 mM putrescine or spermine for 1 h) significantly improved green plant regeneration per 100 ELS of only two ICAR-DA genotypes. ELS treated with polyamines for 30 min were greener and formed more adventitious roots. The chloroplasts of these greener ELS examined with a transmission electron microscope had agranal to grana thylakoids, while those of the control had plastids with mostly starch globules. Although exogenous application of polyamines to anthers improved the production of ELS and green plants, the effects of putrescine, spermidine, and spermine was dependent on genotype and the duration of pretreatment of anthers with the polyamines.

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Section: Introductionmentioning

confidence: 99%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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“…Additionally, the position of the spike on the plant can affect the quality of regenerated plantlets in terms of albinism. However growing conditions can be better controlled in growth chambers so that the number of treatments needed to avoid diseases is lower; and furthermore, different temperatures and controlled fertilizers can be used for the donor plants (Cistué and Kasha 2005). Górecka et al (2005) showed that each donor plant of carrot can respond in a different way to anther culture.…”

Section: Introductionmentioning

confidence: 99%

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Cistué

Romagosa

Batlle³

et al. 2009

Plant Cell Rep

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“…The concentration, combination and exogenous hormones balance in the medium are critical. Growth condition of donor plants and stresses to them may greatly affect the endogenous hormone levels and subsequently the hormone levels required in embryo induction medium (Cistue & Kasha, 2005). Profitable effects of PAA have been reported on barley and wheat androgenesis.…”

Section: The Effect Of Embryo Induction Mediummentioning

confidence: 99%

The Evaluation of Response to Isolated Microspores Culture in Some Iranian Hexaploid Wheat (Triticum aestivum L.) Cultivars

Ekhveh¹,

Moieni²,

Javaran³

2013

JAS

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The most important method of haploid plant production is isolated microspores culture. To be successful in this method, genetic potential of cultivars is very important. In this research, ten Iranian hexaploid wheat cultivars (exclusive of MV17) on the basis of their characters such as heat, drought and salinity resistant have been selected and their responses to isolated microspore culture were evaluated. Meanwhile the effects of two pretreatments (cold and heat + chemical) and embryo induction media (NPB99 and CHB-2) on androgenic traits including embryogenesis, total plant regeneration and green plant regeneration were studied in selected cultivars. There were significant interactions between cultivars and pretreatments and also between cultivars and media in most cases. The interaction between cultivars Chamran and Navid with 21 days cold pretreatment produced the highest number of embryos and the interaction between cultivar Hamoon and 21 days cold and heat + chemical pretreatments produced the most green plant regeneration percentage. Also, the results showed that the interaction between cultivar Chamran and CHB-2 medium produced the highest number of embryos while the interaction between cultivar Hamoon and NPB99 medium had the highest green plant regeneration.

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Gametic Embryogenesis in Triticum: a Study of Some Critical Factors in Haploid (Microspore) Embryogenesis

Cited by 7 publications

References 70 publications

Effects of exogenous application of polyamines on wheat anther cultures

Effects of exogenous application of polyamines on wheat anther cultures

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

The Evaluation of Response to Isolated Microspores Culture in Some Iranian Hexaploid Wheat (Triticum aestivum L.) Cultivars

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