Gametosomatic hybridization between egg cell protoplast and mesophyll protoplast of Petunia hybrida

Sangthong, Ratchada; Chin, Dong Poh; Supaibulwatana, Kanyaratt; Mii, Masahiro

doi:10.5511/plantbiotechnology.26.377

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Isolation of ovules from the ovary is a very cumbersome procedure, with risk of damage to ovary and contamination from somatic tissue. Using a procedure developed for isolating female germ unit (FGU) from ovules of Petunia (Sangthong et al, 2009a), Sangthong et al (2009b) successfully reported gametosomatic hybridization by using female gametophyte (egg cell) as the gametic-haploid partner instead of male gametophyte in Petunia hybrida. More recently, Skálová et al (2012) reported isolation of haploid and mixoploid protoplasts in cucumber (Cucumis sativus L.; 2x/4x; 2x = 14).…”

Section: Gametosomatic Hybridizationmentioning

confidence: 98%

Haploids: Constraints and opportunities in plant breeding

Dwivedi

Britt

Tripathi

et al. 2015

Biotechnology Advances

235

134

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The discovery of haploids in higher plants led to the use of doubled haploid (DH) technology in plant breeding. This article provides the state of the art on DH technology including the induction and identification of haploids, what factors influence haploid induction, molecular basis of microspore embryogenesis, the genetics underpinnings of haploid induction and its use in plant breeding, particularly to fix traits and unlock genetic variation. Both in vitro and in vivo methods have been used to induce haploids that are thereafter chromosome doubled to produce DH. Various heritable factors contribute to the successful induction of haploids, whose genetics is that of a quantitative trait. Genomic regions associated with in vitro and in vivo DH production were noted in various crops with the aid of DNA markers. It seems that F2 plants are the most suitable for the induction of DH lines than F1 plants. Identifying putative haploids is a key issue in haploid breeding. DH technology in Brassicas and cereals, such as barley, maize, rice, rye and wheat, has been improved and used routinely in cultivar development, while in other food staples such as pulses and root crops the technology has not reached to the stage leading to its application in plant breeding. The centromere-mediated haploid induction system has been used in Arabidopsis, but not yet in crops. Most food staples are derived from genomic resources-rich crops, including those with sequenced reference genomes. The integration of genomic resources with DH technology provides new opportunities for the improving selection methods, maximizing selection gains and accelerate cultivar development. Marker-aided breeding and DH technology have been used to improve host plant resistance in barley, rice, and wheat. Multinational seed companies are using DH technology in large-scale production of inbred lines for further development of hybrid cultivars, particularly in maize. The public sector provides support to national programs or small-medium private seed for the exploitation of DH technology in plant breeding.

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Section: Gametosomatic Hybridizationmentioning

confidence: 98%

Haploids: Constraints and opportunities in plant breeding

Dwivedi

Britt

Tripathi

et al. 2015

Biotechnology Advances

235

134

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“…Enzyme treatment in particular has been used to generate protoplasts from the female gametophytes of a wide range of plant species including Ginkgo biloba, Salvinia natans, Laminaria digitata, Petunia hybrida (Laurain et al 1993, Nakamura and Maeda 1994, Benet et al 1997, Sangthong et al 2009, and Torenia fournieri (Mol 1986, Chen et al 2008.…”

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confidence: 99%

Isolation of Gametophytic Cells and Identification of Their Cell-Specific Markers in Torenia fournieri, T. concolor and Lindernia micrantha

et al. 2011

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Summary Gametophytic cells in the female embryo sac, the egg, the central and the synergid cells have unique roles in sexual reproduction in higher plants. To investigate their cell-specific features, an efficient method of distinguishing and isolating each gametophytic cell is required. By testing the activities of several cellulase enzymes, we modified our previous cell isolation method and succeeded in isolating each cell from the Torenia and Lindernia species. We also established genetic markers that show specific expression patterns in each gametophytic cell. These markers, together with the cell isolation method, will be useful for analyzing the function of gametophytic cells.

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Double Haploid: An Overview

Kumar¹,

Choudhary²

2020

Int.J.Curr.Microbiol.App.Sci

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Gametosomatic hybridization between egg cell protoplast and mesophyll protoplast of Petunia hybrida

Cited by 3 publications

References 26 publications

Haploids: Constraints and opportunities in plant breeding

Haploids: Constraints and opportunities in plant breeding

Isolation of Gametophytic Cells and Identification of Their Cell-Specific Markers in Torenia fournieri, T. concolor and Lindernia micrantha

Double Haploid: An Overview

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