Gamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models

Costa, Andrea da; Zorgi, Nahiara Esteves; Nascimento, N.; Júnior, Andres Jimenez Galisteo; Júnior, Heitor Franco de Andrade

doi:10.1016/j.biopha.2018.06.155

Cited by 15 publications

(10 citation statements)

References 36 publications

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“…This may have been due to structural changes in specific epitopes on the toxin molecule. Similar results were also found in crotoxin [65] and total protein extract of Toxoplasma gondii [66] in which these antigens show conserved antigenic and immunological properties after the radiation process.…”

Section: Discussionsupporting

confidence: 81%

Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

Sartori

Costa

Macarini

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of 60 Co. Methods: Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results: Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin

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Section: Discussionsupporting

confidence: 81%

Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

Sartori

Costa

Macarini

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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“…This remarkably effective primary tumor defense is activated and orchestrated by modulation of tumor-associated myeloid cells by T gondii, which results in the reduction of tumor-associated immune suppression, thus facilitating the activation of substantial antitumor immune responses. 2 An effective immunotherapy recently reported for known tumors is that induced by the attenuated nonreplicating T gondii. 4 Previous studies demonstrated immunotherapeutic potency of treatment with nonreplicating attenuated T gondii uracil auxotroph (CPS) that can reverse tumor-associated immunosuppression.…”

Section: Introductionmentioning

confidence: 99%

Gamma Radiation-Attenuated Toxoplasma gondii Provokes Apoptosis in Ehrlich Ascites Carcinoma-Bearing Mice Generating Long-Lasting Immunity

Hafez

Moawed

Abdel-Hamid

et al. 2020

Technol Cancer Res Treat

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Purpose: Pathological angiogenesis and apoptosis evasions are common hallmarks of cancer. A different approach to the antitumor effect of parasitic diseases caused by certain protozoans and helminthes had been adopted in recent years as they can affect many cancer characteristics. The present work is an attempt to assess the effect of gamma radiation-attenuated Toxoplasma gondii ME49 as an antiapoptotic and angiogenic regulator modifier on tumor growth aimed at improving cancer protective protocols. Methods: Attenuated Toxoplasma gondii ME49 was administered orally to mice 2 weeks before inoculation with Ehrlich ascites carcinoma to allow stimulation of the immune response. Hepatic histopathology and immune responses were determined for each group. Results: Marked suppression of the tumor proliferation with induction of long-lasting immunity by stimulating interferon γ and downregulating transforming growth factor β. The level of tumor promoting inflammatory markers (STAT-3 and tumor necrosis factor α), the angiogenic factors (vascular endothelial growth factor A, integrin, and matrix metallopeptidase 2 and matrix metallopeptidase 9), as well as nitric oxide concentration were significantly decreased. This was collimated with an improvement in apoptotic regulators (cytochrome-c, Bax, Bak, and caspase 3) in liver tissues of vaccinated mice group compared to Ehrlich ascites carcinoma-bearing one. Moreover, the histopathological investigations confirmed this improvement. Conclusion: Hence, there is an evidence of potency of radiation attenuated Toxoplasma vaccine in immune activation and targeting tumor cell that can be used as a prophylactic or an adjuvant in combination with chemotherapeutic drugs.

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“…Yoon et al7 have found that PDT with ALA-hx (hexenyl ester of 5-aminolaevulinic acid) more efficiently produced reactive oxygen species (ROS) and suppressed MCF-7/ADR cells viability; the findings suggest that ALA-hx PDT may be used as a therapeutic alternative for drug-resistant breast cancer 7. In addition, the combination of PDT and chemotherapy always has potential advantages compared with traditional treatments, such as high selectivity, minimal invasiveness, and effectiveness in overcoming chemotherapy drug resistance 8–10…”

Section: Introductionmentioning

confidence: 99%

<p>Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy</p>

Wang¹,

Li²,

Li³

et al. 2019

IJN

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BackgroundDrug resistance is one of the prime reasons of chemotherapy failure in breast cancer and is also an important factor affecting prognosis.PurposeIn this study, we constructed a functional magnetic mesoporous silica-based nanocomposite (MMSN) for breast cancer chemotherapy/photodynamic therapy.MethodsMMSN was characterized by scanning electron microscopy and transmission electron microscopy to observe the morphology. The size distribution and zeta potential of the MSNs were determined using Malvern Particle Size Analyzer. Anti-tumor activity in vitro was investigated by CCK-8 assay, flow cytometry and transwell experiment, and the anti-tumor activity in vivo was probed into by magnetic targeting, toxicity, and antitumor effects in breast cancer-bearing BABL/c nude mice.ResultsThe results showed that the release of doxorubicin in the nanocomposites was pH sensitive, and the cumulative release rate reached 80.53% at 60 h under acidic conditions. The nanocomposites had a high cellular uptake ability in MCF-7/ADR cells, and the IC50 value of the nanocomposites on MCF-7/ADR cells was 4.23 μg/mL, much smaller than that of free DOX (363.2 μg/mL). The nanocomposites could effectively reverse resistance and induce apoptosis of MCF-7/ADR cells. The blood biochemistry parameters and H&E staining results showed no serious adverse effects after treatment with the nanocomposites. Prussian blue staining showed that the nanocomposites were able to target tumor tissues in tumor-bearing mice under a magnetic field. The combined chemical/photodynamic therapy significantly inhibited tumor growth in vivo.ConclusionNanocomposites with magnetic and pH dual-responsive performance has shown a promising platform for enhanced drug-resistant breast cancer treatment.

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Gamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models

Cited by 15 publications

References 36 publications

Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

Gamma Radiation-Attenuated Toxoplasma gondii Provokes Apoptosis in Ehrlich Ascites Carcinoma-Bearing Mice Generating Long-Lasting Immunity

<p>Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy</p>

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