Gamma ray induced genetic changes in different organs of chick embryo using peripheral blood micronucleus test and comet assay

Malladi, S.M.; Bhilwade, Hari N.; Khan, Muhammad Zargham; Chaubey, R.C.

doi:10.1016/j.mrgentox.2007.02.002

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…The dose range between 45 -85 Gy the sensitivity of kidney was observed to be more than that of the liver tissue and lastly the blood. This result at low frequencies is in good agreement to some extent with the findings of [8] which showed se- The interchange in sensitivity of liver samples and kidney tissue at higher frequencies ( Figure 2) may be due to changes in polarization of counter ions near charged surfaces in tissue and possibly, the polarization of the large membrane bound structures and electrolytes [5].…”

Section: Methodssupporting

confidence: 89%

A Comparison of Gamma Irradiation Response Models of Bovine Blood, Liver and Kidney Tissues at Radiofrequency

Sombo¹,

Agba²,

Ige³

et al. 2018

OJBIPHY

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This study is aimed at identifying gamma irradiated response of bovine blood, liver and kidney tissues at radiofrequency. For this purpose, impedence meter (Booton 7200) working in conjunction with signal generators (Loadster, SG416013 and Harris G857993) and improvised parallel plate dielectric cells constructed in line with the method of Laogun et al., (2005) were used to obtain the dielectric spread parameter gamma radiation α, of blood, liver and kidney tissues exposed to gamma irradiation dose range of 1.0-85.0 Gy. The dielectric spread parameter α gives the extent of damage induced in an irradiated tissue. Results of this work revealed that at 0-50 MHz frequency range, Kidney tissues displayed higher sensitive, followed Liver tissues and lasted the bovine blood between 0-60 Gy but reversed for blood and liver at 85 Gy. At 0-100 kHz frequency range liver tissue is more venerable to radiation injuries between gamma irradiation dose range of 0-20 Gy while between 43-85 Gy the Kidney's sensitivity is the highest followed by blood and liver tissues. This implies that the liver tissues are less liable to radiation injuries at radiofrequency. A comparison of the linear, exponential and polynomial models using Akaike Information Criteria (AIC) revealed that linear models were the most suitable models for describing the effect of gamma radiation on the dielectric dispersion properties of bovine tissues at low and high radiofrequencies. This implies that the response of the investigated tissues increases linearly with gamma irradiation dose.

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Section: Methodssupporting

confidence: 89%

A Comparison of Gamma Irradiation Response Models of Bovine Blood, Liver and Kidney Tissues at Radiofrequency

Sombo¹,

Agba²,

Ige³

et al. 2018

OJBIPHY

View full text Add to dashboard Cite

show abstract

“…Langthasa et al (2022) showed an increase in DNA damage of peripheral blood in chick embryos exposed to BPA. Using a comet assay, Malladi et al (2007) also demonstrated that BPA is potentially enough to induce DNA damage in blood. It was found that BPA can cause genetic damage in estrogen receptors (ERs) and its genotoxicity is dependent on hormone receptors expression.…”

Section: Ggt Enzymementioning

confidence: 99%

Genotoxic Effect and Oxidative Damage in the Chicken Embryo Caused by Bisphenol A

Al-Sharifi,

Mohammed,

Alsultan

2023

Intern. J. Zool. Invest.

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The present study aimed to evaluate the Bisphenol A (BPA) effect on chicken embryos. 240 fertilized eggs of Ross 308 were divided into eight groups (n=30 for each), including two control groups. Embryos were injected on day 2 with 100 µl of increasing concentrations of BPA then dissected on days 21-22. The livers were removed from the chicken to evaluate DNA damage (using the PCR technique) and oxidative stress biomarkers. The results showed that BPA induces DNA damage and extensively increases oxidative stress. These confirmed that BPA can affect embryos at early stages by alteration in the genetic materials and changing liver enzymes.

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Gamma irradiation‐induced oxidative stress and developmental impairment in the hermaphroditic fish, Kryptolebias marmoratus embryo

Rhee

Kim

Kang

et al. 2012

Enviro Toxic and Chemistry

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This study investigated the effects of gamma radiation on the early developmental stages in hermaphroditic fish embryos of Kryptolebias marmoratus. The authors measured reactive oxygen species (ROS) level and antioxidant enzyme activities with the endpoint hatching rate after gamma irradiation of different embryonic stages. Then, the transcriptional changes of antioxidant enzyme-coding genes were evaluated by quantitative real-time reverse transcription polymerase chain reaction in response to gamma radiation on embryonic stages. Gamma radiation inhibited hatching rate and caused developmental impairment in a dose-dependent manner. Embryos showed tolerances in a developmental stage-dependent manner, indicating that early embryonic stages were more sensitive to the negative effects of gamma radiation than were later stages. After 5 Gy rate of radiation, the ROS level increased significantly at embryonic stages 2, 3, and 4 with a significant induction of all antioxidant enzyme activities. The expressions of glutathione S-transferase isoforms, catalase, superoxide dismutase (Mn-SOD, Cu/Zn-SOD), glutathione reductase, and glutathione peroxidase mRNA were upregulated in a dose-and-developmental stage-dependent manner. This finding indicates that gamma radiation can induce oxidative stress and subsequently modulates the expression of antioxidant enzyme-coding genes as one of the defense mechanisms. Interestingly, embryonic stage 1 exposed to gamma radiation showed a decreased expression in most antioxidant enzyme-coding genes, suggesting that this is also related to a lower hatching rate and developmental impairment. The results of this study provide a better understanding of the molecular mode of action of gamma radiation in aquatic organisms.

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Gamma ray induced genetic changes in different organs of chick embryo using peripheral blood micronucleus test and comet assay

Cited by 9 publications

References 38 publications

A Comparison of Gamma Irradiation Response Models of Bovine Blood, Liver and Kidney Tissues at Radiofrequency

A Comparison of Gamma Irradiation Response Models of Bovine Blood, Liver and Kidney Tissues at Radiofrequency

Genotoxic Effect and Oxidative Damage in the Chicken Embryo Caused by Bisphenol A

Gamma irradiation‐induced oxidative stress and developmental impairment in the hermaphroditic fish, Kryptolebias marmoratus embryo

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