GammaProteobacteria as a potential bioindicator of a multiple contamination by polycyclic aromatic hydrocarbons (PAHs) in agricultural soils

Niepceron, Maïté; Martin-Laurent, Fabrice; Crampon, Marc; Portet‐Koltalo, Florence; Akpa‐Vinceslas, Marthe; Legras, Marc; Bru, David; Bureau, Fabrice; Bodilis, Josselin

doi:10.1016/j.envpol.2013.05.040

Cited by 85 publications

(43 citation statements)

References 34 publications

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“…Interestingly, the portion of HMW PAH increased with increasing total PAH content, while LMW PAHs showed a contrary trend in the examined soils. Some studies and the results here have shown that microbial populations involved in PAH degradation may be enriched by PAH pollution (Laurie and Lloyd-Jones 2000;Niepceron et al 2013), and it is also well known that HMW PAHs are more resistant to degradation by soil microbes than LMW PAHs (Johnsen et al 2005;Lors et al 2010). So a higher proportion of LMW PAHs may be eliminated in seriously polluted soils than in slightly polluted soils, and consequently, HMW PAHs tend to accumulate in seriously polluted soils (Johnsen et al 2006).…”

Section: N4(6)mentioning

confidence: 57%

“…Moreover, there was a very significant correlation between the nah gene abundance and PAH content. The close relationship between Gammaproteobacteria and Betaproteobacteria and PAH in soils has been reported in recent studies, especially Gammaproteobacteria has been suggested as a bioindicator of PAH pollution (Lors et al 2010;Niepceron et al 2013). Lors et al (2010) monitored the PAHdegrading bacterial community structures by PCR and clone library in a coal-tar-contaminated soil in the process of windrow treatment.…”

mentioning

confidence: 76%

“…Thus, the Gammaproteobacteria were suggested as a bioindicator of the potential biodegradation of PAHs in contaminated soil. Niepceron et al (2013) monitored the abundances of ten bacterial phyla and classes by qPCR and Pseudomonas isolates using direct culture in soil microcosms spiked with a mixture of seven PAHs over a 90-day p e r i o d a n d f o u n d t h a t B e t a p r o t e o b a c t e r i a a n d Gammaproteobacteria were more sensitive to the PAH mixture than other bacterial groups. Abundance of the Betaproteobacteria was significantly higher compared to the controls only at 60 days of incubation, while abundances of the Gammaproteobacteria and culturable Pseudomonas (affiliated to the Gammaproteobacteria) significantly increased over the longer period of incubation in response to the addition of PAH mixture.…”

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confidence: 99%

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Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Han

Liu

Zheng

et al. 2014

Environ Sci Pollut Res

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Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by realtime quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.

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Section: N4(6)mentioning

confidence: 57%

mentioning

confidence: 76%

mentioning

confidence: 99%

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Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Han

Liu

Zheng

et al. 2014

Environ Sci Pollut Res

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“…Even so, most of C12O clones were still related to Gammaproteobacteria and Betaproteobacteria. It indicated that Gammaproteobacteria and Betaproteobacteria could be important microbial degraders of PAHs in soils [3,40]. PERM ANOVA analyses on T-RFLP profiles showed that addition of DOMs and incubation time exerted the significant influence on community compositions of both nah and C12O genes.…”

Section: Discussionmentioning

confidence: 99%

“…Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous chemical pollutants which can pose a threat to human health and ecosystem safety as a result of their recalcitrant, mutagenic, and carcinogenic properties and high bioaccumulation of the food chain [1][2][3]. Soil is the main natural reservoir of PAHs; thereby, remediation of the PAH-contaminated soil has received a lot of scientific attention in recent years [4].…”

Section: Introductionmentioning

confidence: 99%

Insight into the Modulation of Dissolved Organic Matter on Microbial Remediation of PAH-Contaminated Soils

et al. 2015

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Microorganisms play a key role in degradation of polycyclic aromatic hydrocarbons (PAHs) in environments. Dissolved organic matter (DOM) can enhance microbial degradation of PAHs in soils. However, it is not clear how will the soil microbial community respond to addition of DOM during bioremediation of PAH-contaminated soils. In this study, DOMs derived from various agricultural wastes were applied to remediate the aging PAH-contaminated soils in a 90-day microcosm experiment. Results showed that the addition of DOMs offered a more efficient and persistent elimination of soil PAHs compared to the control which had no DOM addition. PAH removal effects were different among treatments with various DOMs; the addition of DOMs with high proportion of hydrophobic fraction could remove PAHs more efficiently from the soil. Low-molecular-weight (LMW) PAHs were more easily eliminated than that with high-molecularweight (HMW). Addition of DOMs significantly increased abundance of 16S ribosomal RNA (rRNA), pdo1, nah, and C12O genes and obviously changed community compositions of nah and C12O genes in different ways in the PAHcontaminated soil. Phylogenetic analyses of clone libraries exhibited that all of nah sequences and most of C12O sequences were affiliated into Gammaproteobacteria and Betaproteobacteria. These results suggested that external stimuli produced by DOMs could enhance the microbial degradation of PAHs in soils through not only solubilizing PAHs but also altering abundance and composition of indigenous microbial degraders. Our results reinforce the understanding of role of DOMs in mediating degradation of PAHs by microorganims in soils.

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Role of Pet Dogs and Cats as Sentinels of Human Exposure to Polycyclic Aromatic Hydrocarbons

Rial-Berriel

Henríquez-Hernández

Luzardo

2019

Pets as Sentinels, Forecasters and Promoters of Human Health

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GammaProteobacteria as a potential bioindicator of a multiple contamination by polycyclic aromatic hydrocarbons (PAHs) in agricultural soils

Cited by 85 publications

References 34 publications

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area

Insight into the Modulation of Dissolved Organic Matter on Microbial Remediation of PAH-Contaminated Soils

Role of Pet Dogs and Cats as Sentinels of Human Exposure to Polycyclic Aromatic Hydrocarbons

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