Ganglioside from eel serum high density lipoprotein (HDL) and its role as a ligand for HDL binding protein

Kumagai, Akiko; Chisada, Shin ichi; Kamei, Yuto; Ito, Makoto; Hayashi, Seiichi

doi:10.1016/j.cbpb.2007.04.007

Cited by 3 publications

(5 citation statements)

References 24 publications

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“…The binding of FITC-HDL to the hepatocytes was inhibited in the presence of 50 times excess of non-labeled HDL. These results show that FITC-HDL bound specifically to cultured hepatocytes (Kumagai et al 2007).…”

Section: -4a Stimulatory Effect Of Hdl On Vldl-like Lipoprotein Synmentioning

confidence: 53%

“…24). These results suggest that neither apoAI nor AII functions as the ligand of eel HDL receptor (Kumagai et al 2007).…”

Section: -4b Apoai and Apoaii Of Hdl Do Not Function As A Ligand Fomentioning

confidence: 71%

“…Gangliodside GM4 was purified from lyophilized eel serum HDL, as reported by Kumagai et al (2007). The mobilities of the purified GM4 and its lyso-form were the same as those of authentic GM4 from human brain and its lyso-form on HPTLC analysis.…”

Section: -4c-1 Ganglioside Gm4 Isolated From Eel Serum Hdlmentioning

confidence: 72%

“…Although there are reports on gangliosides of serum lipoproteins in human serum (Chatterjee and Kwiterovich 1976;Clark 1980), the report on ganglioside GM4 of eel HDL is the first one in Teleostei (Kumagai et al 2007). In human serum the major gangliosides of serum HDL are gangliosides GM3, GD3, and GM2, but GM4 is not detected (Senn et al 1989).…”

Section: -4c-1 Ganglioside Gm4 Isolated From Eel Serum Hdlmentioning

confidence: 95%

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Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Hayashi¹,

Kumagai²

2008

Aqua-BioSci. Monogr. (ABSM)

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Perfused eel livers, isolated eel hepatocytes, and cultured eel hepatocytes are used to investigate eel liver functions such as gluconeogenesis, glycogen synthesis, and lipoprotein synthesis and methods for preparation are described. A novel phosphoenolpyruvate (PEP) synthesis pathway from pyruvate in gluconeogenesis in eel liver was elucidated and PEP synthesis pathways in eel, rat, and pigeon livers were compared. Glycogen synthesis from pyruvate, lactate, and glucose was investigated by using cultured eel hepatocytes. It was found that 10-6 or 10-7 M glucagon didn't stimulate glycogen degradation in the presence of pyruvate but stimulated glycogen degradation in the presence of lactate. Glycogen synthesis from pyruvate was observed even when 10-6 or 10-7 M glucagon was present. The characteristics of the lipoprotein synthesized and secreted by cultured eel hepatocytes are clarified. Thyroxine and eel serum high-density lipoprotein (HDL) stimulated the lipoprotein synthesis by cultured eel hepatocytes. In the presence of estradiol-17β, eel serum HDL stimulated vitellogenin synthesis in eel hepatocytes. HDL specifically bound to eel hepatocytes and the ligand of HDL receptor in the plasma membrane of eel hepatocytes was identified to be ganglioside GM4 of eel serum HDL.

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Section: -4a Stimulatory Effect Of Hdl On Vldl-like Lipoprotein Synmentioning

confidence: 53%

“…24). These results suggest that neither apoAI nor AII functions as the ligand of eel HDL receptor (Kumagai et al 2007).…”

Section: -4b Apoai and Apoaii Of Hdl Do Not Function As A Ligand Fomentioning

confidence: 71%

Section: -4c-1 Ganglioside Gm4 Isolated From Eel Serum Hdlmentioning

confidence: 72%

Section: -4c-1 Ganglioside Gm4 Isolated From Eel Serum Hdlmentioning

confidence: 95%

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Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Hayashi¹,

Kumagai²

2008

Aqua-BioSci. Monogr. (ABSM)

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“…Differences in the expression of GSL-glycans between serum lipoproteins and RBCs should be investigated in future studies. Kumagai et al reported that the ligand for the eel serum HDL-binding protein is GM4 of HDL, but little is known about the functions of GSLs in human serum. Blood group-specific glyco-epitopes were abundant on GSLs in both RBCs and serum lipoproteins.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Glycomic Approach Reveals Novel Low-Molecular-Weight Blood Group-Specific Glycans in Serum and Cerebrospinal Fluid

et al. 2021

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ABO blood antigens on the human red blood cell membrane as well as different cells in various human tissues have been thoroughly studied. Anti-A and -B antibodies of IgM are present in serum/plasma, but blood group-specific glyco-antigens have not been extensively described. In this study, we performed comprehensive and quantitative serum glycomic analyses of various glycoconjugates and free oligosaccharides in all blood groups. Our comprehensive glycomic approach revealed that blood group-specific antigens in serum/plasma are predominantly present on glycosphingolipids on lipoproteins rather than glycoproteins. Expression of the ABO antigens on glycosphingolipids depends not only on blood type but also on secretor status. Blood group-specific glycans in serum/plasma were classified as type I, whereas those on RBCs had different structures including hexose and hexosamine residues. Analysis of free oligosaccharides revealed that low-molecular-weight blood group-specific glycans, commonly containing lacto-N-difucotetraose, were expressed in serum/plasma according to blood group. Furthermore, comprehensive glycomic analysis in human cerebrospinal fluid showed that many kinds of free oligosaccharides were highly expressed, and low-molecular-weight blood group-specific glycans, which existed in plasma from the same individuals, were present. Our findings provide the first evidence for low-molecular-weight blood group-specific glycans in both serum/plasma and cerebrospinal fluid.

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Molecular Marker–Assisted Selective Breeding of Largemouth Bass

Bai¹,

Li²

2019

Genetic Breeding and Molecular Marker-Assisted Selective Breeding of Largemouth Bass

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Ganglioside from eel serum high density lipoprotein (HDL) and its role as a ligand for HDL binding protein

Cited by 3 publications

References 24 publications

Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Studies on Eel Liver Functions Using Perfused Liver and Primary Cultured Hepatocytes

Comprehensive Glycomic Approach Reveals Novel Low-Molecular-Weight Blood Group-Specific Glycans in Serum and Cerebrospinal Fluid

Molecular Marker–Assisted Selective Breeding of Largemouth Bass

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