Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction

Abstract

Background: Ganoderma lucidum has been widely used as a herbal medicine for promoting health and longevity in China and other Asian countries. Polysaccharide extracts from Ganoderma lucidum have been reported to exhibit immuno-modulating and anti-tumor activities. In previous studies, F3, the active component of the polysaccharide extract, was found to activate various cytokines such as IL-1, IL-6, IL-12, and TNF-. This gave rise to our investigation on how F3 stimulates immuno-modulating or anti-tumor effects in human leukemia THP-1 cells.

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

“…Ganoderma lucidum (a medicinal fungi in Asia) has been used for a long time to prevent and treat various human diseases (Lin et al, 2006;Cheng et al, 2007;Hua et al, 2007;Ji et al, 2007;Zhu et al, 2007;Chuang et al, 2009;Chen et al, 2010;Lai et al, 2010). The mainly components of G. lucidum polysaccharides are (1→3) and (1→6)--D-glucan.…”

Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3

“…The anticancer properties of Ganoderma lucidum have been attributed to its bioactive compounds including polysaccharides and a group of triterpenes (15)(16)(17)(18). To date, the molecular details by which Ganoderma lucidum exerts its biological effects remain incompletely understood.…”

Cytotoxicity of New Metal Porphyrinates Based on Chlorophyll a Derivatives

“…Forward Primer Reverse Primer Source BAX AGAGGATGATTGCCGCCGT CAACCACCCTGGTCTTGGAT [16] BBC3 CTGTGAATCCTGTGCTCTGC TCCTCCCTCTTCCGAGATTT [16] TNFSF10 GCTGAAGCAGATGCAGGACAAG CTGACGGAGTTGCCACTTGAC [17] CDKN1A CAGCAGAGGAAGACCATGTG GGCGTTTGGAGTGGTAGAAA [18] CDKN2A GACATCCCCGATTGAAAGAA TTTACGGTAGTGGGGGAAGG Primer BLAST*…”

“…GCTGACGGCTGCATCTGTT CCTGATTTGGACAAGCAGCAA [19] GSR ATCCCCGGTGCCAGCTTAGG AGCAATGTAACCTGCACCAACAA [20] PBP74 TCTGGACTGAATGTGCTTCG ATCCCCATTTGTGGATTTCA [21] GAPDH ACACCCACTCCTCCACCTTTG GCTGTAGCCAAATTCGTTGTCATAC [17] ACTB GCGCGGCTACAGCTTCA CTTAATGTCACGCACGATTTCC [22] *Developed using Primer BLAST online service: http://www.ncbi.nlm.nih.gov/tools/primer-blast/…”