Ganoderma lucidum polysaccharides inhibit in vitro tumorigenesis, cancer stem cell properties and epithelial-mesenchymal transition in oral squamous cell carcinoma

Camargo, Marcela Rodrigues de; Frazon, Talita Fonseca; Inacio, Kelly Karina; Smiderle, Fhernanda Ribeiro; Amôr, Nádia Ghinelli; Dionísio, Thiago José; Santos, Carlos; Rodini, Camila Oliveira; Lara, Vanessa Soares

doi:10.1016/j.jep.2021.114891

Cited by 33 publications

(13 citation statements)

References 88 publications

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“…Feng et al demonstrated that treatment of Eca109 cells with 2 mg/mL dose of Ganoderma lucidum polysaccharides accelerated the apoptosis of Eca109 cells through the mitochondrial pathway with a proliferation inhibition rate of 74.63% [ 47 ]. Gamargo et al revealed that only 10 mg/mL and 5 mg/mL of Ganoderma lucidum polysaccharides significantly reduced tongue squamous cell metabolism, resulting in non-invasive and less proliferative behavior of tumor cells [ 48 ]. Together, these results indicated that DLP120 has a certain potential to inhibit the proliferation of HepG2 cells.…”

Section: Resultsmentioning

confidence: 99%

Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

et al. 2022

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Dandelion (Taraxacum mongolicum Hand.-Mazz.) is a medicinal and edible plant. Dandelion has great development value for its health promoting benefits; additionally, Dandelion grows almost anywhere in the world. In this study, we report the structural characteristics and anti-cancer activity of novel dandelion leaf polysaccharides extracted by pressurized hot water extraction at 120 °C (DLP120) with Mw relative to dextran of 1.64 × 106 Da. Structural analysis indicated that DLP120 is a complex polysaccharide composed of pectin and arabinogalactan. It was mainly composed of arabinose (32.35 mol%) and galactose (44.91 mol%). The main glycosidic linkages of DLP120 were 4-β-D-Galp, 4-α-D-GalpA, T-β-D-Galp, 5-α-L-Araf, 3,5-α-L-Araf, and T-α-L-Araf. In vitro, DLP120 inhibited HepG2 cell proliferation in a dose-dependent manner by inducing cell apoptosis. Cell cycle detection results revealed that DLP120 mainly arrests the cell cycle in S phase. Cells treated with DLP120 displayed obvious apoptotic morphology, including cell volume shrinks and cytoskeleton breaks down. In short, DLP120 has potential as an anti-cancer agent.

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Section: Resultsmentioning

confidence: 99%

Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

et al. 2022

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“…EMT in cancers allows cells to detach from the original tumor tissue and may cause an invasion-metastasis cascade ( 39 ). Various polysaccharides have demonstrated anti-tumor mechanisms by inhibiting EMT, such as Ganoderma lucidum polysaccharides, Huaier polysaccharides and Se-lentinan ( 40 – 42 ).…”

Section: Resultsmentioning

confidence: 99%

A novel acidic polysaccharide from blackened jujube: Structural features and antitumor activity in vitro

2022

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Liver cancer is one of the most common cancers, with increasing trends in incidence and mortality. A novel acidic polysaccharide (BJP-2) obtained from blackened jujube was extracted by hot water followed by chromatographic purification employing DEAE-cellulose 52 and Sephadex G-100 column. And then BJP-2 was identified by SEC-MALLS-RI, GC-MS, methylation and NMR for the following characteristics: molecular weight of 6.42 × 104 Da, monosaccharide composition of glucuronic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), xylose (Xyl), glucuronic acid (GlcA), glucose (Glc), fucose (Fuc) and mannose (Man) with the percentage of 39.78, 31.93, 16.86, 6.43, 1.86, 1.28, 1.02, 0.61, and 0.23%, as well as the main chain of → 5)-α-L-Araf (1 → 4)-β-D-Gal(1 → , T-α-L-Araf (1 → 4)-β-D-Gal(1 → , and → 4)-α-L-6MeGalAp(1 → . The effect of BJP-2 on the apoptosis of HepG2 cells and its anti-tumor mechanism were further explored. The analysis by MTT and flow cytometry showed that BJP-2 suppressed cell proliferation by inducing apoptosis in a concentration-dependent manner. Cell scratching and Transwell revealed that BJP-2 was able to block the invasion and metastasis of tumor cells. Western blot results demonstrated that BJP-2 exhibited antitumor activity through a mitochondria-dependent pathway, as evidenced by overexpression of Bax, Cleaved Caspase-3/Caspase-3 and Cleaved Caspase-9/Caspase-9 and downregulation of Bcl-2. Therefore, BJP-2 has broad research prospects as a tumor preventive or therapeutic agent.

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“…The presence of cancer stem cells (CSCs) is responsible for tumor chemoresistance and recurrence ( Gaggianesi et al, 2021 ). GLP suppressed the proliferation, metabolic activity and migration of oral squamous cell carcinoma (OSCC) ( de Camargo et al, 2022 ). Importantly, GLP impeded CSC properties and eventually enhanced the therapeutic responsiveness of OSCC cells by lowering the expression of CSC, EMT and chemosensitivity markers (e.g., BMI1, p75NGFR, twist, N-cadherin, and ABCB1) ( de Camargo et al, 2022 ).…”

Section: The Antitumor Activities Of Polysaccharides From G...mentioning

confidence: 99%

“…GLP suppressed the proliferation, metabolic activity and migration of oral squamous cell carcinoma (OSCC) ( de Camargo et al, 2022 ). Importantly, GLP impeded CSC properties and eventually enhanced the therapeutic responsiveness of OSCC cells by lowering the expression of CSC, EMT and chemosensitivity markers (e.g., BMI1, p75NGFR, twist, N-cadherin, and ABCB1) ( de Camargo et al, 2022 ). GLP might act as therapeutic co-adjuvants to promote CSC elimination, hence suppressing OSCC malignancy and chemosensitizing OSCC cells to traditional treatments.…”

Section: The Antitumor Activities Of Polysaccharides From G...mentioning

confidence: 99%

Research Progress on the Anticancer Activities and Mechanisms of Polysaccharides From Ganoderma

Wang

2022

Front. Pharmacol.

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Cancer ranks as a primary reason for death worldwide. Conventional anticancer therapies can cause severe side effects, and thus natural products may be promising drug candidates for cancer therapy. Accumulating evidence has verified the prominent anticancer properties of Ganoderma polysaccharides, suggesting that Ganoderma polysaccharides may be effective chemopreventive agents of natural origin. Based on their abilities to prevent cancer development by regulating the DNA damage response, cancer cell proliferation, apoptosis, host immunity, gut microbiota and therapeutic sensitivity, there has been increasing interest in elucidating the clinical implication of Ganoderma polysaccharides in cancer therapy. In this review, we summarize recent findings pertaining to the roles of bioactive polysaccharides from Ganoderma in cancer pathogenesis, discuss the multifarious mechanisms involved and propose future directions for research. A more sophisticated understanding of the anticancer benefits of Ganoderma polysaccharides will be helpful for improving current treatments and developing novel therapeutic interventions for human malignancies.

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Ganoderma lucidum polysaccharides inhibit in vitro tumorigenesis, cancer stem cell properties and epithelial-mesenchymal transition in oral squamous cell carcinoma

Cited by 33 publications

References 88 publications

Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

Structural Characteristic and In-Vitro Anticancer Activities of Dandelion Leaf Polysaccharides from Pressurized Hot Water Extraction

A novel acidic polysaccharide from blackened jujube: Structural features and antitumor activity in vitro

Research Progress on the Anticancer Activities and Mechanisms of Polysaccharides From Ganoderma

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