Gardenia jasminoides Attenuates Allergic Rhinitis-Induced Inflammation by Inhibiting Periostin Production

Pyun, Bo-Jeong; Lee, Joo Young; Kim, Yu Jin; Ji, Kon-Young; Jung, Dong Ho; Park, Ki-Sun; Jo, Kyuhyung; Choi, Susanna; Jung, Myung-A; Kim, Taesoo

doi:10.3390/ph14100986

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…The extent of airway mucopolysaccharide positivity was scored by two independent assessors with a score ranging from 0 (none) to 3 (full of mucin) as described [ 26 , 69 , 70 , 71 ] ( Supplementary Materials ). Hematologic Stain from Abcam, Cambridge, UK (ab150665) was used to stain eosinophils [ 72 , 73 , 74 ].…”

Section: Methodsmentioning

confidence: 99%

Obesity Enhances Non-Th2 Airway Inflammation in a Murine Model of Allergic Asthma

Mohamed,

Amrani

2024

IJMS

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Obese patients with asthma present with aggravated symptoms that are also harder to treat. Here, we used a mouse model of allergic asthma sensitised and challenged to house dust mite (HDM) extracts to determine whether high-fat-diet consumption would exacerbate the key features of allergic airway inflammation. C57BL/6 mice were intranasally sensitised and challenged with HDM extracts over a duration of 3 weeks. The impact of high-fat-diet (HFD) vs. normal diet (ND) chow was studied on HDM-induced lung inflammation and inflammatory cell infiltration as well as cytokine production. HFD-fed mice had greater inflammatory cell infiltration around airways and blood vessels, and an overall more severe degree of inflammation than in the ND-fed mice (semiquantitative blinded evaluation). Quantitative assessment of HDM-associated Th2 responses (numbers of lung CD4+ T cells, eosinophils, serum levels of allergen-specific IgE as well as the expression of Th2 cytokines (Il5 and Il13)) did not show significant changes between the HFD and ND groups. Interestingly, the HFD group exhibited a more pronounced neutrophilic infiltration within their lung tissues and an increase in non-Th2 cytokines (Il17, Tnfa, Tgf-b, Il-1b). These findings provide additional evidence that obesity triggered by a high-fat-diet regimen may exacerbate asthma by involving non-Th2 and neutrophilic pathways.

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Section: Methodsmentioning

confidence: 99%

Obesity Enhances Non-Th2 Airway Inflammation in a Murine Model of Allergic Asthma

Mohamed,

Amrani

2024

IJMS

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“…Zhizi and Shuizhizi are rich in active chemicals, including iridoids (e.g., geniposide, geniposidic acid, shanzhiside, deacetylasperulosidic acid methyl ester, genipin 1-gentiobioside, genipin), pigments (e.g., crocin I, crocin II), organic acids (e.g., caffeic acid, chlorogenic acid, neochlorogenic acid, protocatechuic acid), and flavonoids (e.g., isoquercitrin, rutin) ( Xiao et al., 2017 ; Ye et al., 2022b ; Zhang et al., 2024 ). The abundant chemical components in Zhizi exhibit various pharmacological effects such as hepatoprotection ( Fan et al., 2020 ), anti-inflammatory ( Zhou et al., 2019 ), anti-hyperglycemia ( Zhou et al., 2023 ), anti-depression ( Zou et al., 2021 ), and anti-allergy ( Pyun et al., 2021 ) activities. Zhizi is commonly used in traditional Chinese medicine for clearing heart fire, relieving restlessness, eliminating damp heat, and cooling blood detoxification ( Committee for the Pharmacopoeia of PR China, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive quality evaluation of different types of Gardeniae Fructus (Zhizi) and Shuizhizi based on LC-MS/MS

Qian,

Hu,

Wang

et al. 2024

Front. Plant Sci.

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Gardeniae Fructus (Zhizi) serves as both a medicinal and edible substance and finds widespread use in various industries. There are often two kinds of medicinal materials in the market: Zhizi and Shuizhizi. Typically, Zhizi with small, round fruit is used for medicinal purposes, while Shuizhizi, characterized by large, elongated fruit, is employed for dyeing. Market surveys have revealed a diverse range of Zhizi types, and modern research indicates that Shuizhizi contains rich chemical components and pharmacological activities. In this study, we collected 25 batches of Zhizi and Shuizhizi samples, categorizing them based on appearance into obovate and round fruits, with seven length grades (A–G). Using the ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS) method, we simultaneously quantified 13 main chemical components in fruits of Gardenia species. In addition, we compared the weight percentage of the pericarp, flesh, and seeds parts of samples with different traits, and quantified 13 chemical components in different parts. Results indicated that, aside from a few instances of overlapping fruit size ranges, Shuizhizi generally exhibits larger and longer dimensions than Zhizi. The weight proportion of the Shuizhizi pericarp is often higher than that of the Zhizi pericarp. Quantitative results highlighted significant differences in the chemical component content between Zhizi and Shuizhizi, with Shuizhizi generally containing higher levels of iridoids. The PCA and OPLS-DA analysis distinctly divided Shuizhizi and Zhizi, among which three iridoids, two organic acids, and one flavonoid made significant contributions to their classification. Cluster heatmap analysis also demonstrated complete separation between Zhizi and Shuizhizi, with clear distinctions among Zhizi samples from different origins. The distribution of the 13 chemical components in different Zhizi and Shuizhizi parts remained consistent, with iridoids and pigments concentrated in the seeds and flesh, and two organic acids and one flavonoid enriched in the pericarp. In summary, this study contributes valuable insights for classifying Zhizi and offers guidance on the rational use of Shuizhizi and the different parts of Zhizi.

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Hederagenol improves multiple sclerosis by modulating Th17 cell differentiation

Guan,

Li,

Zhao

et al. 2024

IUBMB Life

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Multiple sclerosis (MS) is a common autoimmune illness that is difficult to treat. The upregulation of Th17 cells is critical in the pathological process of MS. Hederagenol (Hed) has been shown to lower IL‐17 levels, although its role in MS pathophysiology is uncertain. In this study, we explore whether Hed could ameliorate MS by modulating Th17 cell differentiation, with the goal of identifying new treatment targets for MS. The experimental autoimmune encephalomyelitis (EAE) mouse model was conducted and Hed was intraperitoneally injected into mice. The weight was recorded and the clinical symptom grade was assessed. Hematoxylin‐eosin staining was carried out to determine the extent of inflammation in the spinal cord and liver. The luxol Fast Blue staining was performed to detect the pathological changes in the myelin sheath. Nerve damage was detected using NeuN immunofluorescence staining and terminal deoxynucleotidyl transferase dUTP nick‐end labeling staining. Immunohistology approaches were used to study alterations in immune cells in the spinal cord. The proportions of T cell subsets in the spleens were analyzed by flow cytometry. RORγt levels were measured using quantitative real‐time PCR or Western blot. The activity of the RORγt promoter was analyzed by Chromatin immunoprecipitation. Hed administration reduced the clinical symptom grade of EAE mice, as well as the inflammatory infiltration, demyelination, and cell disorder of the spinal cord, while having no discernible effect on the mouse weight. In addition, Hed treatment significantly reduced the number of T cells, particularly Th17 cells in the spinal cord and spleen‐isolated CD4+ T cells. Hed lowered the RORγt levels in spleens and CD4+ T cells and overexpression of RORγt reversed the inhibitory effect of Hed on Th17 differentiation. Hed decreased nerve injury by modulating Th17 differentiation through the RORγt promoter. Hed regulates Th17 differentiation by reducing RORγt promoter activity, which reduces nerve injury and alleviates EAE.

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Gardenia jasminoides Attenuates Allergic Rhinitis-Induced Inflammation by Inhibiting Periostin Production

Cited by 3 publications

References 45 publications

Obesity Enhances Non-Th2 Airway Inflammation in a Murine Model of Allergic Asthma

Obesity Enhances Non-Th2 Airway Inflammation in a Murine Model of Allergic Asthma

Comprehensive quality evaluation of different types of Gardeniae Fructus (Zhizi) and Shuizhizi based on LC-MS/MS

Hederagenol improves multiple sclerosis by modulating Th17 cell differentiation

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