Gardenia jasminoides extract without crocin improved atopic dermatitis-like skin lesions via suppression of Th2-related cytokines in Dfe-induced NC/Nga mice

Park, Sun Haeng; An, Jeong Eun; Jang, Seol; Kim, Jung Young; Lee, Jin Wook; Kim, Ho Kyoung

doi:10.1016/j.jep.2019.112015

Cited by 21 publications

(11 citation statements)

References 33 publications

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“…Importantly, the central pathophysiology in AD is the generation of allergen-specific IgE, most of which is Th2-mediated. We previously reported that GJExCR treatment improved AD skin symptoms by enhancing skin barrier factors in DfE-induced NC/Nga mice [ 18 ]. In the present study, we demonstrated the effect of GJExCR treatment on an OVA-induced allergic reaction.…”

Section: Discussionmentioning

confidence: 99%

“…GJExCR shows β-Hexosaminidase activity and possible anti-inflammatory action in mast cells GJE contains geniposide, which inhibits airway inflammation and hyperresponsiveness in mice through p38 MAPK activation [ 17 ]. Our previous work has shown that although GJExCR treatment inhibits AD through increasing skin barrier function in Dermatophagoides farinae extract (DfE)-induced NC/Nga mice [ 18 ], one component of GJE, crocine, has no effect on AD [ 18 , 19 ]. Here, we further investigated the effectiveness of GJE after removal of the crocin and the mechanism of action for GJExCR protection in regulation of CD4+ and CD8+ cells, and the Th2 related cytokine response during the development of an allergic response in OVA-induced mice.…”

Section: Introductionmentioning

confidence: 99%

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Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Park

Song

et al. 2023

Cells

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A key feature of an allergic immune response is a T helper type 2 (Th2)-mediated response with production of allergen-specific IgE antibodies. Gardenia jasminoides extract with the crocin removed (GJExCR) has been shown to inhibit IgE-mediated allergic disease. To evaluate the efficacy and mechanism-of-action of this inhibition, GJExCR was used in an ovalbumin (OVA)-induced allergy model in BALB/C mice. Sensitization of BALB/C mice with OVA and aluminum hydroxide was performed on days 1 and 14 by intraperitoneal injection, followed by OVA challenge to the dorsal skin for 2 weeks before removal. Seven days post-challenge, mice were treated with GJExCR topically every day for 11 days. Enzyme-linked immunosorbent assay, flow cytometry analysis, real-time PCR, and western blot were performed to determine IgE and Th2 cytokine levels. Following OVA challenge, Th2 cytokine expression and both total and OVA-specific serum IgE levels increased, of which OVA-specific IgE and Th2 cytokine levels decreased after GJExCR treatment. Flow cytometry analysis revealed that GJExCR treatment decreased CD4+ and CD8+ T cell populations in the spleen and lymph nodes. In addition, treatment with GJExCR downregulated signal transducer and activator of transcription 1 (STAT1) activation and Th2 cytokine levels as compared to control. GJExCR containing geniposide downregulated STAT1 activation in HaCaT cells. These findings demonstrate that GJExCR exerts its anti-allergy effect via inhibition of STAT1 activation, thus regulating the immune response via modulation of Th2 cytokine release and IgE levels. Therefore, we propose GJExCR as a potential treatment for allergic hypersensitivity reactions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Park

Song

et al. 2023

Cells

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“…Applying the dried fruit of this plant from Rubiaceae family, practitioners attempt to reduce inflammatory symptoms which result from hepatic disorders. Experimental approaches reported its hepatoprotective [44], anti-inflammatory effects in diseases such as allergy [45][46][47], pancreatitis [48], and endothelial inflammation [49]. Furthermore, numerous reports provide evidence for the anti-cancer effects of GJ and its constituents.…”

Section: Discussionmentioning

confidence: 99%

Gardenia jasminoides Enhances CDDP-Induced Apoptosis of Glioblastoma Cells via AKT/mTOR Pathway While Protecting Death of Astrocytes

Kim

Hong

et al. 2020

Nutrients

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Gliomas are the most observed primary brain tumor, of which glioblastoma multiform (GBM) shows the highest incidence. Radiotherapy with temozolomide is the standard therapeutic method, but because of side effects, search for alternative therapies is required. Gardenia jasminoides (GJ) is flavonoid abundant with beneficial effects on inflammation, metabolic diseases, and cancers. In this study, we investigated the synergistic combination of GJ and cisplatin (CDDP) in U87MG and U373MG GBM cells. GJ and CDDP both showed cytotoxicity in U87MG cells, however GJ did not affect viability of normal astrocytes while CDDP displayed high toxicity. Cytotoxic effect of GJ and CDDP was related in apoptosis when confirmed by Western blot assays on cleaved caspase-3, caspase-9, and PARP. Moreover, GJ and CDDP showed synergistic combination in cell death of GBM cells, which was further confirmed by Western blot assays of apoptosis factors and also flow cytometry of Annexin V. Analysis on autophagy factors showed that GJ/CDDP combination induced autophagy, and through inhibition of autophagy, we could confirm autophagy is crucial to cytotoxicity of GJ/CDDP in GBM cell lines. The autophagy-mediated apoptosis of GJ/CDDP was dependent on the AKT/mTOR pathway. Overall, our results suggest GJ/CDDP combination as an effective yet safe therapeutic approach to GBMs.

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“…Gardenia and its active chemical constituents, such as genipin, have been extensively studied [5]. In particular, numerous pharmacological studies have examined GF for its anti-inflammatory activity and therapeutic effects on patients with allergic diseases [2,3,6,16,17]. Most of these studies have examined how GF and Fig.…”

Section: Discussionmentioning

confidence: 99%

Gardenia jasminoides extract and its constituent, genipin, inhibit activation of CD3/CD28 co-stimulated CD4⁺ T cells via ORAI1 channel

Kim

Nam

Woo

et al. 2020

Korean J Physiol Pharmacol

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Gardenia jasminoides (GJ) is a widely used herbal medicine with antiinflammatory properties, but its effects on the ORAI1 channel, which is important in generating intracellular calcium signaling for T cell activation, remain unknown. In this study, we investigated whether 70% ethanolic GJ extract (GJ EtOH) and its subsequent fractions inhibit ORAI1 and determined which constituents contributed to this effect. Whole-cell patch clamp analysis revealed that GJ EtOH (64.7% ± 3.83% inhibition at 0.1 mg/ml) and all its fractions showed inhibitory effects on the ORAI1 channel. Among the GJ fractions, the hexane fraction (GJ HEX , 66.8% ± 9.95% at 0.1 mg/ml) had the most potent inhibitory effects in hORAI1-hSTIM1 co-transfected HEK293T cells. Chemical constituent analysis revealed that the strong ORAI1 inhibitory effect of GJ HEX was due to linoleic acid, and in other fractions, we found that genipin inhibited ORAI1. Genipin significantly inhibited I ORAI1 and interleukin-2 production in CD3/ CD28-stimulated Jurkat T lymphocytes by 35.9% ± 3.02% and 54.7% ± 1.32% at 30 μM, respectively. Furthermore, the same genipin concentration inhibited the proliferation of human primary CD4 + T lymphocytes stimulated with CD3/CD28 antibodies by 54.9% ± 8.22%, as evaluated by carboxyfluorescein succinimidyl ester assay. Our findings suggest that genipin may be one of the active components of GJ responsible for T cell suppression, which is partially mediated by activation of the ORAI1 channel. This study helps us understand the mechanisms of GJ in the treatment of inflammatory diseases.

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Gardenia jasminoides extract without crocin improved atopic dermatitis-like skin lesions via suppression of Th2-related cytokines in Dfe-induced NC/Nga mice

Cited by 21 publications

References 33 publications

Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Gardenia jasminoides Enhances CDDP-Induced Apoptosis of Glioblastoma Cells via AKT/mTOR Pathway While Protecting Death of Astrocytes

Gardenia jasminoides extract and its constituent, genipin, inhibit activation of CD3/CD28 co-stimulated CD4⁺ T cells via ORAI1 channel

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Gardenia jasminoides extract without crocin improved atopic dermatitis-like skin lesions via suppression of Th2-related cytokines in Dfe-induced NC/Nga mice

Cited by 21 publications

References 33 publications

Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice

Gardenia jasminoides Enhances CDDP-Induced Apoptosis of Glioblastoma Cells via AKT/mTOR Pathway While Protecting Death of Astrocytes

Gardenia jasminoides extract and its constituent, genipin, inhibit activation of CD3/CD28 co-stimulated CD4+ T cells via ORAI1 channel

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Gardenia jasminoides extract and its constituent, genipin, inhibit activation of CD3/CD28 co-stimulated CD4⁺ T cells via ORAI1 channel