Gas to protein: Microbial single cell protein is an alternative to fishmeal in aquaculture

Woolley, L.D.; Chaklader, Reaz; Pilmer, Luke; Stephens, Frances; Wingate, Catherine; Salini, Michael; Partridge, Gavin J.

doi:10.1016/j.scitotenv.2022.160141

Cited by 16 publications

(9 citation statements)

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“…The fermenter unit, also known as the bioreactor, is the production chain core of neoteric biotechnological facilities producing significant products for sustainable development by means of biosynthesis, e.g., single cell proteins [27]. Performing essentially as a mass transfer apparatus, the fermenters are designed to carry out the process of microorganism cultivation [28] with the ability to work on high pressure regimes (up to 6 and above atmospheres) [29,30].…”

Section: Methodology For Conducting the Verification Base For The Mod...mentioning

confidence: 99%

Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms

et al. 2023

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Development of energy-efficient and high-performance bioreactors requires progress in methods for assessing the key parameters of the biosynthesis process. With a wide variety of approaches and methods for determining the phase contact area in gas–liquid flows, the question of obtaining its accurate quantitative estimation remains open. Particularly challenging are the issues of getting information about the mass transfer coefficients instantly, as well as the development of predictive capabilities for the implementation of effective flow control in continuous fermentation both on the laboratory and industrial scales. Motivated by the opportunity to explore the possibility of applying classical and non-classical computer vision methods to the results of high-precision video records of bubble flows obtained during the experiment in the bioreactor vessel, we obtained a number of results presented in the paper. Characteristics of the bioreactor’s bubble flow were estimated first by classical computer vision (CCV) methods including an elliptic regression approach for single bubble boundaries selection and clustering, image transformation through a set of filters and developing an algorithm for separation of the overlapping bubbles. The application of the developed method for the entire video filming makes it possible to obtain parameter distributions and set dropout thresholds in order to obtain better estimates due to averaging. The developed CCV methodology was also tested and verified on a collected and labeled manual dataset. An onwards deep neural network (NN) approach was also applied, for instance the segmentation task, and has demonstrated certain advantages in terms of high segmentation resolution, while the classical one tends to be more speedy. Thus, in the current manuscript both advantages and disadvantages of the classical computer vision method (CCV) and neural network approach (NN) are discussed based on evaluation of bubbles’ number and their area defined. An approach to mass transfer coefficient estimation methodology in virtue of obtained results is also represented.

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Section: Methodology For Conducting the Verification Base For The Mod...mentioning

confidence: 99%

Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms

et al. 2023

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“…β-glucans, nucleotides) and their co-products (e.g. organic acids) can improve immune resistance and digestive tract function of aquatic animals (Glencross et al 2020;Woolley et al 2023).…”

Section: Fermented Ingredients/microbial Proteinsmentioning

confidence: 99%

Aquaculture nutrition in Australia: challenges and trends

et al. 2023

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This review provides an outline of some of the challenges facing nutritionists in the Australian aquaculture industry. It commences with a brief description of how aquaculture nutrition differs from that for terrestrial livestock – the challenges of providing nutrition in aqueous environments, the diversity of species and the high trophic level of most. Specific challenges of aquaculture nutrition are then discussed in further detail, including the difficulty of measuring feed intake and digestibility, the limited ability to use carbohydrates for carnivores, and the specific requirements of species for lipids, including cholesterol, phospholipids, long-chain polyunsaturated fatty acids and carotenoids. The review then examines how environmental, social and governance concerns are leading to new trends in nutrition for the Australian industry. This includes topics such as the replacement of wild-caught fish meal and fish oil, in terms of both sources of omega-3 lipids and protein. For the former, possible solutions include greater use of seafood trimming, algal oil, and GMO-derived products. For the latter, solutions can include use of livestock render, plant protein meals, fermented products, and insects. Nutrient discharge is also a concern for the industry and nutritionist can assist by improving digestibility and nutrient retention from feeds. Finally, the carbon footprint of aquaculture is leading to new directions for industry and, in turn, for the field of aquaculture nutrition.

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“…<75 g) is 25-30 g MJ −1 (Glencross, 2006). There has been limited testing of SCP bacterial meals in barramundi (Delamare-Deboutteville et al, 2019;Woolley et al, 2023) and other carnivorous marine fishes (Aas et al, 2007;Yu et al, 2023). A SCP meal generated from a mixed culture of purple phototrophic bacteria (PPB) (Rhodopseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

“…to 100% FM replacement compared with the control diet (Delamare-Deboutteville et al, 2019). A recent study by Woolley et al (2023) tested a SCP meal derived from M. capsulatus (StringBio Pty, Ltd., Bengaluru, India) at inclusion levels of 25%-75% of FM replacement in juvenile barramundi (average starting weight 89 g). Significant improvements in growth performance and feed utilization were observed over the 31-day trial at the highest inclusion level equal to 30% of total diet (75% FM replacement) relative to the reference diet (Woolley et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

“…A recent study by Woolley et al (2023) tested a SCP meal derived from M. capsulatus (StringBio Pty, Ltd., Bengaluru, India) at inclusion levels of 25%-75% of FM replacement in juvenile barramundi (average starting weight 89 g). Significant improvements in growth performance and feed utilization were observed over the 31-day trial at the highest inclusion level equal to 30% of total diet (75% FM replacement) relative to the reference diet (Woolley et al, 2023). The studies by Delamare-Deboutteville et al (2019) and Woolley et al (2023) differed in the origin source of the SCP incorporated in the test diets, the age/size of the barramundi and had contrasting outcomes with respect to growth performance and survival.…”

Section: Introductionmentioning

confidence: 99%

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Replacement of fishmeal with a microbial single‐cell protein induced enteropathy and poor growth outcomes in barramundi (Lates calcarifer) fry

Samsing,

Sullivan,

Truong

et al. 2024

Journal of Fish Diseases

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Fish meal (FM) replacement is essential for the sustainable expansion of aquaculture. This study focussed on the feasibility of replacing FM with a single‐cell protein (SCP) derived from methanotrophic bacteria (Methylococcus capsulatus, Bath) in barramundi fry (Lates calcarifer). Three isonitrogenous and isoenergetic diets were formulated with 0%, 6.4% and 12.9% inclusion of the SCP, replacing FM by 0%, 25% and 50%. Barramundi fry (initial body weight 2.5 ± 0.1 g) were fed experimental diets for 21 days to assess growth performance, gut microbiome composition and gut histopathology. Our findings revealed that both levels of SCP inclusion induced detrimental effects in barramundi fry, including impaired growth and reduced survival compared with the control group (66.7% and 71.7% survival in diets replacing FM with SCP by 25% and 50%, respectively; p < .05). Both dietary treatments presented mild necrotizing enteritis with subepithelial oedema and accumulation of PAS positive, diastase resistant droplets within hepatocytes (ceroid hepatopathy) and pancreatic atrophy. Microbiome analysis revealed a marked shift in the gut microbial community with the expansion of potential opportunistic bacteria in the genus Aeromonas. Reduced overall performance in the highest inclusion level (50% SCP) was primarily associated with reduced feed intake, likely related to palatability issues, albeit pathological changes observed in gut and liver may also play a role. Our study highlights the importance of meticulous optimization of SCP inclusion levels in aquafeed formulations, and the need for species and life‐stage specific assessments to ensure the health and welfare of fish in sustainable aquaculture practices.

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Gas to protein: Microbial single cell protein is an alternative to fishmeal in aquaculture

Cited by 16 publications

References 50 publications

Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms

Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms

Aquaculture nutrition in Australia: challenges and trends

Replacement of fishmeal with a microbial single‐cell protein induced enteropathy and poor growth outcomes in barramundi (Lates calcarifer) fry

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