Gastrodin alleviates bone damage by modulating protein expression and tissue redox state

Zheng, Bowen; Shi, Chunling; Muhammed, Fenik Kaml; He, Jia; Abdullah, Adil O.

doi:10.1002/2211-5463.12991

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…GAS has been widely reported to exhibit beneficial effects in a variety of neurological diseases and psychiatric disorders, such as Alzheimer's disease, Parkinson's disease and affective disorders, by inhibiting oxidation, inflammation and apoptosis, suppressing microglial activation and regulating mitochondrial cascades (35). Although a previous study revealed the protective effect of GAS on bone-related diseases (36), research into the specific effects and underlying mechanisms of action of GAS in LPS-induced hPDLSC injury is unknown to the best of our knowledge. The present study demonstrated that GAS could protect hPDLSCs against LPS-induced decrease in cell viability.…”

Section: Discussionmentioning

confidence: 99%

Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression

Feng¹

2022

Exp Ther Med

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Periodontitis is a common inflammatory gum disease that destroys the periodontal tissue. Gastrodin (GAS) is the predominant bioactive component of Gastrodia elata Blume and exhibits anti-inflammatory, anti-apoptotic and antioxidant effects in various diseases, including bone-related diseases. The aim of the present study was to investigate whether GAS could protect lipopolysaccharide (LPS)-treated human periodontal ligament stem cells (hPDLSCs) against injury and inflammation, and to determine the potential underlying mechanisms. hPDLSCs were treated with LPS and GAS, alone or in combination, and cell viability, inflammation, oxidative stress levels and apoptosis were analyzed using a Cell Counting Kit-8 assay, ELISA assay, western blotting and flow cytometry, respectively. The osteogenic differentiation capacity was evaluated using an alkaline phosphatase (ALP) assay and Alizarin Red S staining. Sirtuin 3 (SIRT3) was silenced in cells treated with LPS and GAS to verify the involvement of SIRT3 in the effects of GAS. The results demonstrated that LPS-induced decrease in cell viability was rescued by treatment with 1, 10 or 50 µM GAS. The LPS-induced production of proinflammatory cytokines and increased level of oxidative stress were also inhibited following treatment with 50 µM GAS. Furthermore, GAS significantly promoted ALP activity, increased the number of mineralized nodules and increased the expression of proteins involved in osteogenic differentiation, including ALP, Runx2, osteocalcin and osteopontin, after osteoinduction, which were all downregulated following LPS stimulation. In addition, GAS prevented LPS-induced cell apoptosis and restored the imbalance of anti-apoptotic and proapoptotic proteins in hPDLSCs. In addition, SIRT3 knockdown significantly inhibited the protective effect of GAS on LPS-induced hPDLSC injury. In summary, the findings form the present study suggested that GAS may protect hPDLSCs from LPS-induced inflammation, apoptosis and oxidative stress, as well as promote their osteogenic differentiation. The effect of GAS on the osteogenic differentiation of hPDLSCs may therefore depend on the upregulated expression of SIRT3.

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Section: Discussionmentioning

confidence: 99%

Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression

Feng¹

2022

Exp Ther Med

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“…Modern medical research has shown that it not only has antioxidant, anti-inflammatory, and anti-apoptotic properties, but it also improves microcirculation, protects neurons, lowers blood pressure, and prevents osteonecrosis, among other functions. Currently, gastrodin is mostly used for the treatment of cardiovascular, cerebrovascular, and neuropsychiatric diseases [9,16,[53][54][55][56] (Figure 2). decline in bone strength and mass, with age, is temporally associated with in tosis of bone cells such as osteoblasts.…”

Section: Gastrodinmentioning

confidence: 99%

“…In vivo OVX BALB/c female mice ↑Trabecular microstructure, ↑Mineral apposition rate, ↓MDA, ↑GSH [37] In vivo High glycolipid + Streptozocin SD rats ↑Trabecular microstructure, ↓MDA, ↑SOD [68] In vivo Sodium fluoride Wistar rats ↑Trabecular microstructure, ↓MDA, ↑CAT [9] In vitro LPS hPDLSCs ↑Osteogenic differentiation, ↓ROS, ↓MDA, ↓ LDH, ↑SIRT3…”

Section: Gastrodin For Osteoporosis Treatmentmentioning

confidence: 99%

“…Chinese herbal medicines have the advantages of multi-site action, multi-targeted action, high safety, large dosing space, less toxic side effects, and systemic conditioning [8]. Gastrodin, epimedium, curcumin, and other herbal active ingredients have received attention, and their antioxidant, anti-inflammatory, and osteogenic properties have been proven in numerous studies [9][10][11][12]. Compared to other herbal components such as epimedium and curcumin, gastrodin has a relatively simple chemical structure and comprises polyhydroxy functional groups, which are more conducive to chemical polymerization [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Mechanism and Prospect of Gastrodin in Osteoporosis, Bone Regeneration, and Osseointegration

Li¹,

Li²

2022

Pharmaceuticals

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Gastrodin, a traditional Chinese medicine ingredient, is widely used to treat vascular and neurological diseases. However, recently, an increasing number of studies have shown that gastrodin has anti-osteoporosis effects, and its mechanisms of action include its antioxidant effect, anti-inflammatory effect, and anti-apoptotic effect. In addition, gastrodin has many unique advantages in promoting bone healing in tissue engineering, such as inducing high hydrophilicity in the material surface, its anti-inflammatory effect, and pro-vascular regeneration. Therefore, this paper summarized the effects and mechanisms of gastrodin on osteoporosis and bone regeneration in the current research. Here we propose an assumption that the use of gastrodin in the surface loading of oral implants may greatly promote the osseointegration of implants and increase the success rate of implants. In addition, we speculated on the potential mechanisms of gastrodin against osteoporosis, by affecting actin filament polymerization, renin–angiotensin system (RAS) and ferroptosis, and proposed that the potential combination of gastrodin with Mg2+, angiotensin type 2 receptor blockers or artemisinin may greatly inhibit osteoporosis. The purpose of this review is to provide a reference for more in-depth research and application of gastrodin in the treatment of osteoporosis and implant osseointegration in the future.

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“…The PON-1 rs662 polymorphism was associated with higher enzymatic activity, which may lead to different disease susceptibility (18). Studies reported that CAT and SOD enzyme activities decreased in a fluorosis patient group compared with a control group (19,20). Studies reported evidence that coal-burning fluorosis was related to decreased SOD activity and gene expression (17,21).…”

Section: Introductionmentioning

confidence: 99%

Association Between Antioxidant Nutrients, Oxidative Stress-Related Gene Polymorphism and Skeletal Fluorosis in Guizhou, China

Chen

et al. 2022

Front. Public Health

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BackgroundOxidative stress plays an important role in the pathogenesis of endemic fluorosis. We analyzed associations between oxidative stress-related gene polymorphisms (PON1 rs662, CAT rs769217, rs2300182, and SOD2 rs11968525) and skeletal fluorosis, and examined potential gene–environment interactions with dietary vitamin C, vitamin E, zinc, and selenium intake.MethodsA cross-sectional study was conducted in the Zhijin County, Guizhou Province of China. Skeletal fluorosis was identified according to the Chinese Diagnostic Criteria of Endemic Skeletal Fluorosis. Dietary information was assessed through face-to-face interviews by trained interviewers using a 75-item food frequency questionnaire. The genotype was detected by high throughput TaqMan-MGB RT-PCR technology. Odds ratios (ORs) and 95% CIs were calculated using an unconditional logistic regression model.ResultsIntake of vitamin E, zinc, and selenium was found to be inversely associated with the risk of skeletal fluorosis. The multivariable-adjusted ORs were 0.438 (95% CI: 0.268 to 0.715, P-trend < 0.001) for vitamin E, 0.490 (95% CI: 0.298 to 0.805, P-trend = 0.001) for zinc, and 0.532 (95% CI: 0.324 to 0.873, P-trend = 0.010) for selenium when comparing the highest with the lowest quartile. The relationship for vitamin C was not observed after adjustment for risk factors. Furthermore, participants with PON1 rs662 AA genotype had a significantly decreased risk of skeletal fluorosis compared with those with the GG genotype (OR = 0.438, 95% CI: 0.231 to 0.830). GG + AG genotype carriers were 2.212 times more likely to have skeletal fluorosis than AA carriers (OR = 2.212, 95% CI: 1.197 to 4.090). Compared with AA carriers, AG carriers had a 2.182 times higher risk of skeletal fluorosis (OR = 2.182, 95% CI: 1.143 to 4.163). Although we observed the risk of skeletal fluorosis was higher with a lower intake of antioxidant nutrients, the potential interactions between nutrient intake and genetic polymorphisms were not observed.ConclusionParticipants with a higher intake of vitamin E, zinc, and selenium have a lower likelihood of skeletal fluorosis. In addition, the PON1 rs662 polymorphism is related to skeletal fluorosis.

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Gastrodin alleviates bone damage by modulating protein expression and tissue redox state

Cited by 10 publications

References 35 publications

Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression

Gastrodin attenuates lipopolysaccharide‑induced inflammation and oxidative stress, and promotes the osteogenic differentiation of human periodontal ligament stem cells through enhancing sirtuin3 expression

Mechanism and Prospect of Gastrodin in Osteoporosis, Bone Regeneration, and Osseointegration

Association Between Antioxidant Nutrients, Oxidative Stress-Related Gene Polymorphism and Skeletal Fluorosis in Guizhou, China

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