Gastrointestinal Cancer Markers

Sjögren, Hans; Wahrén, Britta

doi:10.1007/978-1-4612-5808-7_5

Cited by 4 publications

(3 citation statements)

References 103 publications

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“…Among colonic epithelial cells, CSA is expressed predominantly on the cell surface of goblet cells, as detected by immunofluorescence. Furthermore, CSA is present in higher concentrations in colon tumors and in polyps than in normal adult or fetal intestinal tissues (for review, see Sjogren and Wahren, 1982).…”

Section: Dlscussionmentioning

confidence: 99%

“…Tiis observable change in tissue architecture and in intercellular association may reflect biochemical changes in structural molecules as well as changes in antigenic expression of specific molecules within the preneoplastic or transformed cell (Matsushima et al, 1968;Potter et al, 1969). Several heteroantisera and MAbs have been described which are reactive with a spectrum of antigens and immunoreactive with colon carcinomas and/or normal colonic epithelium, (for review see Gold and Goldenberg, 1890;Sjogren and Wahren, 1982). The antigens detected by these antibodies could be divided into 3 groups according to their relative tissue distribution.…”

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confidence: 99%

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Immunological characterization of a novel human colon‐associated antigen (CAA) by a monoclonal antibody

Muraro

Wunderlich

Thor

et al. 1987

Intl Journal of Cancer

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We have generated a monoclonal antibody (MAb), designated anti-CAA (colon-associated antigen), using as immunogen a membrane-enriched fraction of a biopsy from a moderately-differentiated human colonic adenocarcinoma. The molecular weight of this reactive antigen was determined by Western blotting to be greater than 200 kDa. When immunohistochemical techniques were used, MAb anti-CAA reacted with epithelium in the majority of normal, dysplastic and malignant colon specimens tested (greater reactivity was observed in normal colon than in benign or malignant lesions). Cell sorter analyses demonstrated a heterogeneous distribution of CAA on the cell surface of the well-differentiated LS-174T cell line. Antigen positive and antigen-negative cells were separated by means of flow cytometric techniques. These two subpopulations were then inoculated into immunosuppressed rats, resulting in xenograft tumors which differed significantly in their degree of histologic differentiation. Antigen-positive cells developed into well-differentiated adenocarcinomas, while antigen-negative cells developed into poorly differentiated adenocarcinomas. These results, along with immunohistochemical studies, indicate that the antigen detected by MAb anti-CAA has characteristics of a colon-associated antigen whose expression correlates with cellular differentiation. Moreover, differences in molecular weight as well as tissue distribution indicate that CAA may be a novel antigen different from those previously described.

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Section: Dlscussionmentioning

confidence: 99%

mentioning

confidence: 99%

Immunological characterization of a novel human colon‐associated antigen (CAA) by a monoclonal antibody

Muraro

Wunderlich

Thor

et al. 1987

Intl Journal of Cancer

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“…Various cancer markers have been tested for their possible usefulness in complementing clinical, radiological, and histopathological examinations of the intestine in UC (see Sjogren and Wahren, 1982, for review). Thus far, carcinoembryonic antigen (CEA) in serum seems to be limited since serum levels are also increased in cases of benign UC (Loewenstein and Zamcheck, 1978).…”

mentioning

confidence: 99%

Gastrointestinal carcinoma‐associated antigen detected by a monoclonal antibody in dysplasia and adenocarcinoma associated with chronic ulcerative colitis

Olding

Åhrén

Svalander

et al. 1985

Intl Journal of Cancer

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Colorectal tissue specimens from 13 patients with chronic ulcerative colitis, of whom all had epithelial dysplasia and 2 had adenocarcinoma, were tested for the presence of gastrointestinal carcinoma-associated antigen (GICA), using an immunoperoxidase technique with a monoclonal antibody (MAb) against this antigen. GICA was present in the formaldehyde-fixed and paraffin-embedded sections of dysplastic and cancer tissue but absent from normal or hyperplastic epithelium. However, the pattern and extent of staining with the antibody did not correlate with the degree of dysplasia, i.e., "mild" dysplasia was often positive, and "severe" dysplasia was sometimes negative. Changes classified as "indefinite for dysplasia but probably negative" were variable in their expression of GICA. The adenocarcinomas were selectively labelled within cell clusters. In contrast, ulcerative colitis (UC) patients with severe inflammatory changes but with no detectable dysplasia were negative for GICA. GICA could be eluted from paraffin blocks of dysplastic tissue and biochemically characterized as a glycolipid. The detection of this antigen might be a useful complement to morphological examination in discriminating between precancerous and benign epithelial lesions of the colon.

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Urinary modified nucleosides as tumor markers in cancer of the urinary organs or female genital tract

Kawakami¹,

Harmenberg²,

Stendahl

et al. 1985

Urol. Res.

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Using a sensitive and specific method involving high-performance liquid chromatography, urinary levels of four modified nucleosides--pseudouridine (psi), 1-methylinosine (m1I), 1-methyladenosine (m1A), and 1-methylguanosine (m1G)--were investigated before and after treatment in 31 patients with cancer of the urinary organs or the female genital tract. Before treatment m1I was the most frequently elevated nucleoside (77%). Pretreatment urinary levels of psi, m1I, and m1A in patients with stage 2-4 cancer of the female genital tract were significantly elevated compared to human healthy volunteers (p less than 0.005). Compared with the other nucleosides, psi appeared to correlate more closely with the clinical outcome (progression or regression) of patients with cancer of the female genital tract. In the case of patients with cancer of the urinary organs, m1I followed the clinical outcome better than the other nucleosides measured. Therefore psi and m1I seem to be useful for monitoring genito-urinary cancers.

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Gastrointestinal Cancer Markers

Cited by 4 publications

References 103 publications

Immunological characterization of a novel human colon‐associated antigen (CAA) by a monoclonal antibody

Immunological characterization of a novel human colon‐associated antigen (CAA) by a monoclonal antibody

Gastrointestinal carcinoma‐associated antigen detected by a monoclonal antibody in dysplasia and adenocarcinoma associated with chronic ulcerative colitis

Urinary modified nucleosides as tumor markers in cancer of the urinary organs or female genital tract

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