Gastrointestinal Epithelial Renewal

Eastwood, Gregory L.

doi:10.1016/s0016-5085(77)80221-7

Cited by 215 publications

(39 citation statements)

References 147 publications

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“…In the esophagus, the proliferative zone is a basal layer adjacent to the base-ment membrane. In mice, replacement of the esophageal mucosal epithelium takes 7 days [12]. In any event, the intestinal mucosa and esophageal mucosa show rapid renewal.…”

Section: Discussionmentioning

confidence: 99%

Telomerase activity in esophageal carcinoma

et al. 1997

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Background and Objectives Telomerase is a ribonucleoprotein that synthesizes telomeric DNA. Immortalized and carcinoma cells show no loss of telomere length during cell division. Telomerase activity has been demonstrated in carcinomas of various organs, but not in nonneoplastic tissues. In patients with esophageal carcinoma, no data have been reported concerning the relationship between telomerase activity and clinicopathological findings. Materials and Methods Esophageal carcinomas from 31 patients and normal esophageal mucosae from 92 patients were examined. Telomeric Repeat Amplification Protocol assay to detect telomerase activity and Southern blot analysis to examine telomere length were performed. Results Of the 31 carcinomas, 27 (87%) had detectable telomerase activity. Twenty‐one (23%) of the 92 normal esophageal mucosae from autopsied patients also had detectable telomerase activity. There was no difference between stage and outcome and absence or presence of telomerase activity. No difference in terminal restriction fragment (TRF) length was observed between carcinomas with and without telomerase activity. Conclusion Telomerase activity was demonstrated in a considerable number of normal esophageal mucosae. This suggests the possibility of a high frequency of false positivity if the presence of telomerase activity alone is used as a tumor‐specific marker. J. Surg. Oncol. 1997;66:88–92. © 1997 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Telomerase activity in esophageal carcinoma

et al. 1997

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“…[7][8][9][10][11][13][14][15][16][17][18][19][20][21][22][23][24] These values were mostly within the range 20-60 bp per year. In an autoradiographic study in mice, cellular renewal times of hepatocytes, renal tubular and glomerular cells, and splenic tissue were found to be 460-480, 140-190 and 1-21 days, respectively, 25 whereas very rapid renewal was found to occur in epithelial cells of the gastrointestinal mucosae, showing a turnover of within 7 days. 12,25 Previous data we had obtained for annual telomere reduction in gastrointestinal mucosae [9][10][11] showed values lower than 70 bp.…”

Section: Annual Telomere Reduction Rate and Tissue Renewal Timesmentioning

confidence: 94%

“…In digestive tract mucosae, after mitosis, one of two daughter cells migrates up from the proliferative zone to the surface layer and then desquamates into the gastrointestinal lumen within 7 days. 12,25 Hepatocytes in the portal space gradually stream toward the terminal hepatic vein, where they are probably eliminated by apoptosis; this process takes approximately 201 days. 28 Renal cortical cells have never been reported to undergo cellular streaming.…”

Section: Annual Telomere Reduction Rate and Tissue Renewal Timesmentioning

confidence: 99%

Changes of telomere length with aging

Takubo

Aida

Izumiyama-Shimomura

et al. 2010

Geriatrics Gerontology Int

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We reviewed our methodology and results of telomere measurements, with reference to telomere length and aging. Human tissues always showed telomere shortening with age, except for the brain and myocardium. Yearly rates of telomere length reduction in various tissues were mostly within the range 20-60 bp, and thus compatible with that expected from only one round of mitosis. It was suggested that when telomeres were found to be longer in any specific organ in a given individual, then the other organs in that individual would also have longer telomeres. Using the quantitative fluorescence in situ hybridization (Q-FISH) method for telomere measurement, we were able to measure the telomere lengths of various cell types within tissues. Here we summarize the results obtained for various cell types in the stomach, tongue and breast. Our Q-FISH method using our original software program "Tissue Telo" is excellent for measuring telomere lengths using tissue sections and PNA probes. Geriatr Gerontol Int 2010; 10 (Suppl. 1): S197-S206.

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“…enteroendocrine cells), or not at all (e.g. Paneth cells) [33]. Neither explains the lacking increase of 59 Fe absorption 12 h after H 2 O 2 exposure.…”

Section: Discussionmentioning

confidence: 93%