GC-MS analysis and antimicrobial activity of the aqueous extract from the bulbs of Allium chinense G. Don. cultivated in North Sumatra, Indonesia

Naibaho, Frans Grovy

doi:10.35495/ajab.2019.12.562

Cited by 5 publications

(3 citation statements)

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“…A higher abundance of Mortierella was detected in healthy yaks compared with ruminants infected with Cryptosporidium parvum [40]. A lower abundance of Plectosphaerella was examined in fluoride-induced mice [41,42], Tomentella was positively related to the generation of short-chain fatty acids [43][44][45] and Filobasidium reduced ulcerative colitis [46]; the higher abundance of this genus in the EZ group revealed that concentrated feed #3 promoted sheep health by mediating fungi abundance. The genera of Aphanoascus, Gibberella, Thelonectria and Sebacina are commonly reported in the environment [47][48][49][50].…”

Section: Discussionmentioning

confidence: 97%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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The gut microbiome plays an important role in the metabolism, nutrient absorption and immunocompetency of animals. The dynamics of the microbiota can be influenced by modulatory factors that involve nutrition, environment, health, diseases, etc. Few reports have been documented regarding the effects of different feeds on the fungi microbiome of Suffolk crossed with Tibetan sheep. A total of 30 Suffolk crossed with Tibetan sheep (ST sheep) were selected for the study and randomly divided into five equal groups (n = 6): AZ, BZ, CZ, DZ and EZ. Group AZ was fed with alfalfa and oat grass, whereas group BZ was fed with mixture of concentrated feed, alfalfa and oat grass. Groups CZ, DZ and EZ were fed with concentrated feed #1, #2 and #3, respectively. All experimental animals were fed twice a day for four months, and rectum samples were collected for microbiota analysis. Results revealed that 2,781,461 raw reads and 2,333,239 clean reads were achieved in the ST sheep. When compared with the sheep of groups AZ and BZ (164), the shared amplicon sequence variants (ASVs) between AZ and CZ (109), AZ (113) and DZ (118) as well as AZ along with EZ were fewer. Conspicuous different phyla (8) and genera (56) were examined and compared with free-range sheep in AZ. Genera including Xeromyces, Kazachstania, Cordyceps, Rhodotorula, Pichia, Spor, etc. were found higher in animals in the CZ, DZ and EZ groups. The results of this study provide new insights regarding the effects of different feeds on the fungi microbiome of sheep farmed on the plateau. We concluded that the differences in feed in Suffolk crossed with Tibetan sheep altered their gut microbiota.

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Section: Discussionmentioning

confidence: 97%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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“…net), identified by its retention indices (relative to n-alkanes C8-C22) and mass spectrum matching to available authentic standards. Wiley spectral library collection and the National Institute of Standards and Technology (NIST) library database curves generated by running GC analysis of representative authentic compounds 24,27 .…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysis Of P P...mentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

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Natural products play a significant role in providing the current demand as antiparasitic agents, which offer an attractive approach for the discovery of novel drugs. The present study aimed to evaluate in vitro the potential impact of seaweed Padina pavonica (P. pavonica) extract in combating Acanthamoeba castellanii (A. castellanii). The phytochemical constituents of the extract were characterized by Gas chromatography–mass spectrometry. Six concentrations of the algal extract were used to evaluate its antiprotozoal activity at various incubation periods. Our results showed that the extract has significant inhibition against trophozoites and cysts viability, with complete inhibition at the high concentrations. The IC50 of P. pavonica extract was 4.56 and 4.89 µg/mL for trophozoites and cysts, respectively, at 24 h. Morphological alterations of A. castellanii trophozoites/cysts treated with the extract were assessed using inverted and scanning electron microscopes and showed severe damage features upon treatment with the extract at different concentrations. Molecular Docking of extracted compounds against Acanthamoeba cytochrome P450 monooxygenase (AcCYP51) was performed using Autodock vina1.5.6. A pharmacokinetic study using SwissADME was also conducted to investigate the potentiality of the identified bioactive compounds from Padina extract to be orally active drug candidates. In conclusion, this study highlights the in vitro amoebicidal activity of P. pavonica extract against A. castellanii adults and cysts and suggests potential AcCYP51 inhibition.

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“…Cows suffering from metritis have decreased ovarian functionality [6], and it goes to unleash in the presence of bacterial infections like S. aureus and E. coli, as these are common pathogens circulating in humans and animals. Alternatives to antimicrobials have been used to tackle the growing issues of antimicrobial resistance [7]. These pathogens remain a serious concern for public health as metritis is often a sequel of mastitis and thus can sufficiently be harmful to humans who deal with animals or consume milk [8].…”

Section: Introductionmentioning

confidence: 99%

Recent Trends in S. aureus and E. coli-Based Endometritis, and the Therapeutic Evaluation of Sodium Alginate-Based Antibiotics and Nanoparticles

Talib,

Nabeel,

Haq

et al. 2023

Gels

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Postpartum infection of the uterus by pathogenic bacteria is exacerbated due to a lack of sufficient epidemiological studies and evidence-based therapeutics. Therefore, this study was planned to find the prevalence, risk factors, and drug-resistance profile of S. aureus and E. coli isolated from bovine endometritis and to evaluate the antibacterial potential of sodium alginate-based antibiotics and nanoparticles. The study revealed 34.21% S. aureus and 31.57% E. coli, whereas most of the assumed risk factors presented significant association in this study. S. aureus showed the highest resistance against fusidic acid (60%) and cefoxitin (50%), while the highest resistance in E. coli was found against fusidic acid (60%), gentamicin (60%), chloramphenicol (50%), and cefoxitin (50%). Tylosin coupled with MgO nanoparticles stabilized in sodium alginate gel (Tylo + MgO + gel) presented significantly lower minimum inhibitory concentration (MIC) against E. coli, showing 13.88 ± 4.51 µg/mL after 24 h incubation. On the other hand, gel-based preparations showed MIC as 31.25 ± 0 µg/mL (Tylo + gel + MgO) and 26.04 ± 9.02 µg/mL (Tylo + Gel) against S. aureus. Generally, the MICs of non-gel-based preparations were significantly higher against bacteria except ampicillin against S. aureus in this study. The toxicity analysis of MgO nanoparticles presented 20–80% mortality of snails against a wider range of 0.01 mg/mL–10 mg/mL. The histopathological parameters concluded MgO nanoparticles safe to use on off targets. The current study thus concludes the rise in antimicrobial resistance while the gel-based products appearing as effective antimicrobials with sufficient safety margins for off-targets. The study thus invites further investigation for the development of suitable and affordable modified therapeutics for better health and production of animals.

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GC-MS analysis and antimicrobial activity of the aqueous extract from the bulbs of Allium chinense G. Don. cultivated in North Sumatra, Indonesia

Cited by 5 publications

References 0 publications

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Recent Trends in S. aureus and E. coli-Based Endometritis, and the Therapeutic Evaluation of Sodium Alginate-Based Antibiotics and Nanoparticles

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