GC–MS analysis for compound identification in leaf extract of Lumnitzera racemosa and evaluation of its in vitro anticancer effect against MCF7 and HeLa cell lines

Eswaraiah, G.; Peele, K. Abraham; Krupanidhi, S.; Indira, M.; Kumar, Rahul; Venkateswarulu, T.C.

doi:10.1016/j.jksus.2019.01.014

Cited by 25 publications

(10 citation statements)

References 13 publications

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“…Obtained pentacyclic triterpenoid 3β,13β,14β-13, 27-cycloursan-3-ol acetate possesses antibacterial, antioxidant and anticarcinogenic properties [21]. Olean-12-en-3-yl acetate has an antiinflammatory, antidepressant and anticancer activity [22]. Due to the availability of pharmacological active components in L. tatarica roots, it can be recom mended for further investigations onto the respective area.…”

Section: Resultsmentioning

confidence: 99%

Gas chromatography – mass spectrometry analysis of acetone extract of Lactuca tatarica (L.) C. A. Mey. roots

Shukurlu

2022

Experimental Biology and Biotechnology

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Research work is devoted to the chemical investigation of the roots of the Lactuca tatarica (L.) C. A. Mey. by gas chromatography – mass spectrometry method. From the crude extract that is obtained from the roots, the presence of natural compounds of various groups has been detected. As a result, 25 different substances including acyclic compounds (methyl myristate, palmitic acid methyl ester, ethyl palmitate, methyl elaidate, methyl stearate, ethyl oleate, etc.) have been identified from the solid mass of the research object. The most abundant fatty acid esters are methyl oleate (6.831 %) and palmitic acid methyl ester (2.902 %). In addition, the triterpenoid compounds (lupeol acetate, cycloartenol acetate, 3β,13β,14β-13, 27-cycloursan-3-ol acetate) which are characteristic of the L. tatarica have been identified. Lupeol acetate is the predominant component which makes up 56.35 % of the whole obtained substances. Elaidic acid which belongs to the fatty acids group, bis(2-ethylhexyl)phthalate which belongs to the aromatic dicarboxylic acid esters and other compounds have also been identified. Among the identified substances, some are found previously from the other species of the genus Lactuca L. but with regards to the L. tatarica to the best of our knowledge the majority of them are firstly being reported in this study. The investigated plant is widespread in the lowlands and coastal areas of Azerbaijani flora. The availability of some pharmacological effective compounds (lupeol acetate, 3β,13β,14β-13, 27-cycloursan-3-ol acetate, olean-12-en-3-yl acetate) makes the research significant in terms of chemical aspect. Therefore, the results of this investigation open up new horizons for future studies in various fields including the pharmaceutical one.

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Section: Resultsmentioning

confidence: 99%

Gas chromatography – mass spectrometry analysis of acetone extract of Lactuca tatarica (L.) C. A. Mey. roots

Shukurlu

2022

Experimental Biology and Biotechnology

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“…The final crude extract was dissolved in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich Chemicals Pvt Ltd, Merck, Bangalore, India), made to final concentration of 100 mg/mL and used for antiproliferative studies. The concentration of DMSO maintained in the wells was less than 1%, which is not toxic to the cell lines ( 8 , 9 ).…”

Section: Methodsmentioning

confidence: 99%

Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells

Venkateswarulu

Eswaraiah

Krupanidhi

et al. 2020

Food technol. biotechnol. (Online)

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Mangroves contain a wide range of bioactive compounds with pharmacological activities. In the present study, we analysed the separation and detection of phytoconstituents with the methanol extract of Ipomoea tuba leaf using gas chromatography-mass spectrometry (GC-MS) and tested its in vitro cytotoxicity effect against MCF-7 and HeLa cells. Phytochemical compounds such as docosanoic, octadecatrienoic and cis-9-octadecanoic acids, triterpenoid γ-sitosterol, and terpene alcohol in methanol extract of I. tuba leaf were identified. Furthermore, in vitro antiproliferative activity of the extract of I. tuba leaf was evaluated using MCF-7 and HeLa cells. The results indicated a reduction of cell viability of 37.43 and 41.89 % of MCF-7 and HeLa cells respectively. The methanol extract of I. tuba leaf proved to be effective in protecting the cells against oxidative stress. This is the first report on the in vitro cytotoxicity effect of I. tuba leaf extract on MCF-7 and HeLa cells.

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“…Recent research found that chloroquine-sensitive (MRC-2) and chloroquine-resistant (RKL-9) plasmodium falciparum isolates were both significantly inhibited by leaf chloroform and methanol extract, with IC50 values of less than 2 g/ml. [35] . The HepG2 cell line was used to test the anti-cancer properties of four different mangrove plants: Bruguiera gymnorrhiza, Aegiceras corniculatum, Aegialitis rotundifolia, and Lumnitzera racemosa.…”

Section: Anti-hypertensive Activitymentioning

confidence: 99%

Insights into phyto pharmaceutical studies of the under-utilized mangrove species of Lumnitzera racemosa wild: A review

Soumya¹,

Srivani²,

Mohan³

2022

Int. J. Herb. Med.

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The traditional medical system has made extensive use of plants and naturally occurring compounds derived from plants. The plant has been discovered to be phyto chemically abundant in tannins, terpenes, terpenoids, phenolic compounds, phytosterols, and a variety of new metabolites that have displayed notable pharmacological effects. Lumnitzera racemosa wild is a non-viviparous evergreen shrub or small tree, an Indo-West Pacific mangrove. It is extensively used therapeutically and is becoming more wellknown as a valuable medicinal plant. Its morphology, chemical components, and biological activity in connection to extracts and extracted secondary metabolites have all been extensively studied in several examinations of this medicinal plant. This plant is used as anti-inflammatory, anti-angiogenic, anticancer, anti-diabetic, anti-oxidant, anti-hypertensive, anti-bacterial, and Cytotoxic, Hepatoprotective and anti-leishmanial activity. This review summarises the present level of knowledge about morphology, important bioactive components, their chemistry, purported medicinal properties, pharmacological activity, and traditional applications.

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GC–MS analysis for compound identification in leaf extract of Lumnitzera racemosa and evaluation of its in vitro anticancer effect against MCF7 and HeLa cell lines

Cited by 25 publications

References 13 publications

Gas chromatography – mass spectrometry analysis of acetone extract of Lactuca tatarica (L.) C. A. Mey. roots

Gas chromatography – mass spectrometry analysis of acetone extract of Lactuca tatarica (L.) C. A. Mey. roots

Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells

Insights into phyto pharmaceutical studies of the under-utilized mangrove species of Lumnitzera racemosa wild: A review

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