GC MS Analysis of One Ayurvedic Preparation ‘Aswagandharishtam

Kotteswari, M.; Rao, Mudiganti Ram Krishna; Kumar, S Arun; Prabhu, K; Sundaram, R. Lakshmi; Dinakar, Shruthi

doi:10.13005/bpj/1467

Cited by 13 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, hexadecanoic acid methyl ester [39], 9,12,15-octadecatrienoic acid methyl ester, which was present at the highest level relative to the other constituents [42], and methyl stearate [45] were the identified fatty acids methyl esters that are known to exert antioxidant and anticancer effects. However, the fatty acid methyl ester cis-5,8,11-eicosatrienoic acid methyl ester exerts anti-inflammation-related effects [48]. The identified diterpene was phytol, of which there was a considerable amount relative to the other components, and it has antioxidative stress and anticancer activities [43,44].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis and Profiling of Antioxidants and Anticancer Compounds from Tephrosia purpurea (L.) subsp. apollinea Family Fabaceae

2023

View full text Add to dashboard Cite

Tephorosia purpurea subsp. apollinea was extracted with methanol and n-hexane to obtain sub-fractions. The chemical compounds identified with GC-MS and HPLC in T. purpurea subsp. apollinea extracts showed antioxidant and anticancer properties. The antioxidant and anticancer activities were investigated using DDPH and ABTS assays, and MTT assay, respectively. Stigmasta-5,24(28)-dien-3-ol, (3 β,24Z)-, 9,12,15-octadecatrienoic acid methyl ester, phytol, chlorogenic acid, and quercetin were the major chemical compounds detected in T. purpurea subsp. apollinea. These compounds possessed antioxidant and anticancer properties. The methanol extract showed antioxidant properties with DDPH and ABTS radical scavenging of 84% and 94%, respectively, relative to ascorbic acid and trolox. The anticancer effects of T. purpurea subsp. apollinea against the cancer cell lines MCF7 (IC50 = 102.8 ± 0.6 μg/mL), MG63 (IC50 = 118.3 ± 2.5 μg/mL), T47D (IC50 = 114.7 ± 1.0 μg/mL), HeLa (IC50 = 196.3 ± 2.3 μg/mL), and PC3 (IC50 = 117.7 ± 1.1 μg/mL) were greater than its anticancer effects against U379 (IC50 = 248.4 ± 7.5 μg/mL). However, it had no adverse effects on the normal cells (WI38) (IC50 = 242.9 ± 1.8 μg/mL). Therefore, the major active constituents presented in T. purpurea subsp. apollinea can be isolated and studied for their potential antioxidant and anticancer effects against breast, cervical, and prostate cancers and osteosarcoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis and Profiling of Antioxidants and Anticancer Compounds from Tephrosia purpurea (L.) subsp. apollinea Family Fabaceae

2023

View full text Add to dashboard Cite

show abstract

“…Hemidesmus indicus has numerous medicinal properties varying from chemo-preventive, anti-diarrheal, anti-cancerous, wound healing power, immunomodulatory, antioxidant, anti-venoms, antileprotic, diuretic properties etc. (Kotteswari et al, 2018). Previous studies reported in vitro antibacterial activity of H. indicus n-butanolic extract's inhibitory activity against human pathogenic bacterial strains viz.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Leaf, Stem and Root Extracts of Hemidesmus indicus Against Aquatic Pathogens

Nisha¹,

Rosmin²,

Preethi³

et al. 2022

IJZI

View full text Add to dashboard Cite

The present study aimed to assess the antimicrobial activity of Hemidesmus indicus against aquatic pathogens. Hemidesmus indicus is a twining shrub with lactiferous branches that produces numerous bioactive compounds. Plant parts are commonly used in traditional medicine to treat a variety of diseases. A comparative evaluation of the antimicrobial activity of Hexane, Ethyl acetate, Ethanol and water extracts of leaf, stem ad root of the Hemidesmus indicus was tested against the aquatic pathogens including bacteria and fungi by standard agar well diffusion method. The results indicated that all the plant parts exhibited varying degrees of antimicrobial properties. However, among the selected parts the highest antimicrobial property was shown by root against the bacteria Streptococcus agalactiae (16 mm), Streptococcus iniae (12 mm), Aeromonas hydrophila (20 mm), Vibrio cholera (21 mm), Pseudomonas aeruginosa (19 mm), and the fungus Aspergillus niger (19 mm) and Candida albicans (15 mm). Whereas the ethyl acetate fractions of the leaf showed maximum inhibitory activity against aquatic pathogens next to the root and the aqueous extract of the stem exhibited strong inhibition against Vibrio cholera. The study confirmed that the ethyl acetate extract of the Hemidesmus indicus root has high antibacterial activity against fish pathogens meanwhile the ethanol extract exhibited high antifungal activity. The present investigation confirmed the efficacy of Hemidesmus indicus as a natural alternative to synthetic antibiotics making promising contributions to the field of drug discovery and tremendous scope in the field of aquaculture.

show abstract

“…Ashwagandha (winter cherry), is an important medicinal herb in Indian Ayurveda, for more than 3000 years used as indigenous medicinal herb (Murthy et al 2010) and cultivated (Kotteswari et al 2018). Indian ginseng is the other name given based on its reported properties like, sedative, tonic, adaptogens, hypnotic, analgesic, diuretic, antipyretic, abortifacient, and anti-in ammatory (Murthy et al 2010).…”

Section: Introductionmentioning

confidence: 99%

HPTLC Fingerprinting Reveals Leaf and Roots Phytochemical Variability in Developmental stages of Withania somnifera

Lingfa

Baloji

Jagtap

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Withania somnifera has been the foundation of numerous contemporary medicines today for treatment of various diseases. However, phytochemical variability profile is lacking in the root and leaf organs. Aim This study was aimed to elucidate the phytochemical profile in seedling, vegetative and reproductive stages of leaf and roots in W. somnifera using High performance thin layer chromatography (HPTLC) fingerprinting. Methods The HPTLC with dual wavelength UV (254/366 nm) was used for the analysis of phytochemical variation in leaf and root methanolic extracts of W. somnifera. Results HPTLC chromatograms in 366 nm, 254 nm, and normal day light, seedling, vegetative and reproductive stages leaf and root exhibited bands variability. At 450 nm leaf HPTLC fingerprints in seedling stage showed five peaks with Rf values in the range of 0.42 to 0.90, vegetative stage with nine peaks of Rf values in range of 0.10 to 0.89, and reproductive stage with six peaks of Rf values in range of 0.30 to 0.92. In root showed six peaks with Rf values in range of 0.00 to 0.82 in seedling stage, ten peaks with Rf values in range of 0.00 to 0.96 in vegetative stage, seven peaks with Rf values in range of 0.01 to 0.91 in reproductive stage. In roots, higher peak areas were found at the reproductive stage (3.33%) compared to vegetative stage (1.65%). Conclusion The comparative HPTLC fingerprints of leaves and roots in W. somnifera presented phytochemical variability in leaf and root that varied with developmental stages.

show abstract

GC MS Analysis of One Ayurvedic Preparation ‘Aswagandharishtam

Cited by 13 publications

References 45 publications

Phytochemical Analysis and Profiling of Antioxidants and Anticancer Compounds from Tephrosia purpurea (L.) subsp. apollinea Family Fabaceae

Phytochemical Analysis and Profiling of Antioxidants and Anticancer Compounds from Tephrosia purpurea (L.) subsp. apollinea Family Fabaceae

Antimicrobial Activity of Leaf, Stem and Root Extracts of Hemidesmus indicus Against Aquatic Pathogens

HPTLC Fingerprinting Reveals Leaf and Roots Phytochemical Variability in Developmental stages of Withania somnifera

Contact Info

Product

Resources

About