GC-MS and MALDI-TOF MS Profi ling of Sucrose Esters from Nicotiana tabacum and N. rustica

Abstract

Matrix-assisted laser desorption/ionization-time-of-fl ight mass spectrometry (MALDI-TOF MS) has been applied for the fi rst time to the analysis of the sucrose esters from the surface of Nicotiana L. leaves. The profi les obtained for the model plant N. tabacum were similar to those from the gas chromatography-fl ame ionization detector (GC-FID) analysis. The most reproducible results were obtained using a dihydroxybenzoic acid (DHB) matrix. The main advantage of this method is that crude plant extracts can be analysed without sample clean-up. GC-MS analysis of Aztec tobacco (N. rustica) extracts revealed the presence of three types of sucrose esters. All identifi ed compounds had three C 4 − C 8 acyl chains substituting the glucose moiety, while the fructose part of the molecule was substituted with 0, 1, or 2 acetyl groups. MALDI-TOF MS analysis of the sucrose ester fraction revealed the presence of compounds not eluting from a GC column. Combining the data from both GC-MS and MALDI-TOF MS experiments, we obtained a full sucrose ester profi le, which is based on the molecular weight of the compounds and on the number of acyl chains in the molecule.

Characterization of Trichome-Expressed BAHD Acyltransferases in Petunia axillaris Reveals Distinct Acylsugar Assembly Mechanisms within the Solanaceae

“…Secretion of acylsugars creates a sticky exudate that covers the aerial surfaces of plants that is implicated in direct defense against herbivores, tritrophic interactions, and possesses antimicrobial activity (Goffreda et al, 1989;Chortyk et al, 1993;Hare, 2005;Weinhold and Baldwin, 2011;Luu et al, 2017). Acylsugars consist of either a Suc or Glc backbone onto which acyl groups of differing lengths, typically C2 to C12, are esterified at various positions (King et al, 1986(King et al, , 1988Halinski and Stepnowski, 2013;Ghosh et al, 2014). Acyl groups can be straight chain (n) or branched chain, with the latter present in the iso (i) and anteiso (ai) configurations (Ghosh et al, 2014;Liu et al, 2017).…”

Biochemical Characterization and Responses of Two Contrasting Genotypes of Chenopodium quinoa Willd. to Salinity in a Hydroponic System

“…Maximum supernatant was then transferred to 1.5 mL tubes and then centrifuged at 12000 g for 10 minutes at room temperature. An amount of 150 μl supernatant was then transferred to a 2 mL sample vial and placed SpeedVac (Thermo Fisher) vacuum rotary evaporation for 4 h. Methoxypyridine solution {methoxyamine hydrochloride (42 mg) + pyrimidine (2.1 mL)}2(80 μl, 20 mgmL ) for the analysis of the sugars [38,39].…”