GC–MS Based Metabolomics Strategy for Cost-Effective Valorization of Agricultural Waste: Groundnut Shell Extracts and Their Biological Inhibitory Potential

Arumugam, Manohar; Manikandan, Dinesh Babu; Sridhar, Arun; Palaniyappan, Sivagaami; Jayaraman, Sudharshini; Ramasamy, Thirumurugan

doi:10.1007/s12649-022-01768-z

Cited by 8 publications

(4 citation statements)

References 180 publications

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“…They also have lipopolysaccharides that limit the penetration of hydrophobic compounds [55]. Based on our previous results, both GNS and BGP solvent extracts possess phenols and tannins [56,57]. These primary bioactive compounds are the major cause that exhibits better antioxidant and antibacterial properties [58,59].…”

Section: Discussionmentioning

confidence: 92%

“…Groundnut shells (GNS) and black gram pods (BGP) were collected based on their detailed experimental procedures [56,57]. The collected agri-wastes were shade dried at 37 • C, ground into a coarse powder, sieved using 0.2 mm sieve plates, and then stored at −20 • C for subsequent examination in an airtight container.…”

Section: Preparation Of Extractmentioning

confidence: 99%

“…This ligand screening is based on the metabolites identified through GC-MS analysis from various solvent extracts of groundnut shells and black gram pods from our previous study [56,57]. A total of 325 metabolites were identified, of which 14 compounds from groundnut shells and 20 compounds from black gram pods with potential antibacterial efficacy were chosen for this study based on the earlier literature to analyze their interaction with the aerolysin (Table 1).…”

Section: Ligand Screening For Molecular Dockingmentioning

confidence: 99%

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Evaluating Biofilm Inhibitory Potential in Fish Pathogen, Aeromonas hydrophila by Agricultural Waste Extracts and Assessment of Aerolysin Inhibitors Using In Silico Approach

et al. 2023

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Aeromonas hydrophila, an opportunistic bacteria, causes several devastating diseases in humans and animals, particularly aquatic species. Antibiotics have been constrained by the rise of antibiotic resistance caused by drug overuse. Therefore, new strategies are required to prevent appropriate antibiotic inability from antibiotic-resistant strains. Aerolysin is essential for A. hydrophila pathogenesis and has been proposed as a potential target for inventing drugs with anti-virulence properties. It is a unique method of disease prevention in fish to block the quorum-sensing mechanism of A. hydrophila. In SEM analysis, the crude solvent extracts of both groundnut shells and black gram pods exhibited a reduction of aerolysin formation and biofilm matrix formation by blocking the QS in A. hydrophila. Morphological changes were identified in the extracts treated bacterial cells. Furthermore, in previous studies, 34 ligands were identified with potential antibacterial metabolites from agricultural wastes, groundnut shells, and black gram pods using a literature survey. Twelve potent metabolites showed interactions between aerolysin and metabolites during molecular docking analysis, in that H-Pyran-4-one-2,3 dihydro-3,5 dihydroxy-6-methyl (−5.3 kcal/mol) and 2-Hexyldecanoic acid (−5.2 kcal/mol) showed promising results with potential hydrogen bond interactions with aerolysin. These metabolites showed a better binding affinity with aerolysin for 100 ns in molecular simulation dynamics. These findings point to a novel strategy for developing drugs using metabolites from agricultural wastes that may be feasible pharmacological solutions for treating A. hydrophila infections for the betterment of aquaculture.

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Section: Discussionmentioning

confidence: 92%

Section: Preparation Of Extractmentioning

confidence: 99%

Section: Ligand Screening For Molecular Dockingmentioning

confidence: 99%

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Evaluating Biofilm Inhibitory Potential in Fish Pathogen, Aeromonas hydrophila by Agricultural Waste Extracts and Assessment of Aerolysin Inhibitors Using In Silico Approach

et al. 2023

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“…ABTS [2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging activity was determined by cation decolorization assay with slight modifications in Arumugam et al [ 99 ] to analyze the antioxidant potential of various solvent extracts of CR. The ABTS stock solution was prepared by mixing equal volumes of 7 mM ABTS and 140 mM of potassium persulfate solution and allowed them to react in dark conditions at 25 °C for 12–16 h before use.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Phytochemical Screening, Pigment Content, In Vitro Antioxidant, Antibacterial Potential and GC-MS Metabolite Profiling of Green Seaweed Caulerpa racemosa

et al. 2023

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Exploration of seaweeds to unravel their bioactive metabolites from the perspective of wider applications gained substantial importance. The present study was performed to investigate the total phenolic, flavonoid, tannin content, antioxidant activity and antibacterial potential of various solvent extracts of green seaweed Caulerpa racemosa. The methanolic extract showed higher phenolic (11.99 ± 0.48 mg gallic acid equivalents/g), tannin (18.59 ± 0.54 mg tannic acid equivalents/g) and flavonoid (33.17 ± 0.76 mg quercetin equivalents/g) content than other extracts. Antioxidant activity was determined by using 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay with different concentrations of C. racemosa extracts. The methanolic extract showed higher scavenging potential in both the DPPH and ABTS activity with the inhibition value of 54.21 ± 1.39% and 76.62 ± 1.08%, respectively. Bioactive profiling was also identified by using Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared (FT-IR) techniques. These studies revealed the presence of valuable bioactive compounds in C. racemosa extracts and these compounds might be responsible for antimicrobial, antioxidant, anticancer and anti-mutagenic properties. Major compounds identified in GC-MS were 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, 3-hexadecene and Phthalic acid. In terms of antibacterial activity, C. racemosa has promising antibacterial potential against aquatic pathogens Aeromonas hydrophila, Aeromonas veronii and Aeromonas salmonicida. Further evaluation studies focusing aquatic related aspects would reveal the novel bioproperties and applications of C. racemosa.

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Bioactive Analysis of Antibacterial Efficacy and Antioxidant Potential of Aloe barbadensis Miller Leaf Extracts and Exploration of Secondary Metabolites Using GC–MS Profiling

Palaniyappan

Sridhar

Arumugam

et al. 2023

Appl Biochem Biotechnol

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GC–MS Based Metabolomics Strategy for Cost-Effective Valorization of Agricultural Waste: Groundnut Shell Extracts and Their Biological Inhibitory Potential

Cited by 8 publications

References 180 publications

Evaluating Biofilm Inhibitory Potential in Fish Pathogen, Aeromonas hydrophila by Agricultural Waste Extracts and Assessment of Aerolysin Inhibitors Using In Silico Approach

Evaluating Biofilm Inhibitory Potential in Fish Pathogen, Aeromonas hydrophila by Agricultural Waste Extracts and Assessment of Aerolysin Inhibitors Using In Silico Approach

Evaluation of Phytochemical Screening, Pigment Content, In Vitro Antioxidant, Antibacterial Potential and GC-MS Metabolite Profiling of Green Seaweed Caulerpa racemosa

Bioactive Analysis of Antibacterial Efficacy and Antioxidant Potential of Aloe barbadensis Miller Leaf Extracts and Exploration of Secondary Metabolites Using GC–MS Profiling

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