GDNF mRNA in Schwann cells and DRG satellite cells after chronic sciatic nerve injury

Hammarberg, Henrik; Piehl, Fredrik; Cullheim, Staffan; Fjell, Jenny; Hökfelt, Tomas; Fried, K

doi:10.1097/00001756-199603220-00004

Cited by 186 publications

(113 citation statements)

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“…However, a more detailed analysis of ganglia is needed to determine these relationships and the possible existence of cells coexpressing both binding proteins. It is interesting to note that peripheral nerves are strongly positive for GDNFR-␤ mRNA during development, suggesting that Schwann cells, known to produce GDNF (Hammarberg et al, 1996), express both binding proteins but not RET.…”

Section: Discussionmentioning

confidence: 99%

Neurturin and Glial Cell Line-Derived Neurotrophic Factor Receptor-β (GDNFR-β), Novel Proteins Related to GDNF and GDNFR-α with Specific Cellular Patterns of Expression Suggesting Roles in the Developing and Adult Nervous System and in Peripheral Organs

et al. 1997

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Cloning strategies were used to identify a gene termed glial cell line-derived neurotrophic factor receptor-␤ (GDNFR-␤) related to GDNFR-␣. In situ hybridization was then used to map cellular expression of the GDNF-related trophic factor neurturin (NTN) and GDNFR-␤ mRNA in developing and adult mice, and comparisons with GDNFR-␣ and RET were made. Neurturin is expressed in postnatal cerebral cortex, striatum, several brainstem areas, and the pineal gland. GDNFR-␤ mRNA was more widely expressed in the developing and adult CNS, including cerebral cortex, cerebellum, thalamus, zona incerta, hypothalamus, brainstem, and spinal cord, and in subpopulations of sensory neurons and developing peripheral nerves. NTN colocalized with RET and GDNFR-␣ in ureteric buds of the developing kidney. The circular muscle layer of the developing intestines, smooth muscle of the urether, and developing bronchiolae also expressed NTN. GDNFR-␤ was found in myenteric but not submucosal intestinal plexuses. In developing salivary glands NTN had an epithelial expression, whereas GDNFR-␤ was expressed in surrounding tissue. Neurturin and GDNFR-␤ were present in developing sensory organs. In the gonads, NTN appeared to be expressed in Sertoli cells and in the epithelium of the oviduct, whereas GDNFR-␤ was expressed by the germ cell line. Our findings suggest multiple roles for NTN and GDNFR-␤ in the developing and adult organism. Although NTN and GDNFR-␤ expression patterns are sometimes complementary, this is not always the case, suggesting multiple modi operandi of GDNF and NTN in relation to RET and the two binding proteins, GDNFR-␣ and GDNFR-␤.

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Section: Discussionmentioning

confidence: 99%

Neurturin and Glial Cell Line-Derived Neurotrophic Factor Receptor-β (GDNFR-β), Novel Proteins Related to GDNF and GDNFR-α with Specific Cellular Patterns of Expression Suggesting Roles in the Developing and Adult Nervous System and in Peripheral Organs

et al. 1997

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“…Functionally, satellite cells are responsible for maintaining the homeostasis of sensory neurons by regulating extracellular ion and nutrient levels within the DRG (For review see (Hanani, 2005). Following sciatic nerve axotomy in rats, satellite cells have been shown to undergo morphological changes (Woodham et al, 1989;Stephenson & Byers, 1995), proliferate (Shinder & Devor, 1994) and upregulate a variety of growth factors (Hammarberg et al, 1996;Zhou et al, 1999). Additionally, hypertrophy and up-regulation of GFAP in satellite cells has been observed following mechanical injury to the peripheral terminals of sensory neurons that innervate the teeth whose cell bodies are housed within trigeminal ganglia (Stephenson & Byers, 1995).…”

Section: Cellular Alterations In Satellite Cells Macrophages and Schmentioning

confidence: 99%

“…Functionally, macrophage activation within the DRG and peripheral nerve following peripheral nerve injury is known to help remove degenerating neuronal debris and myelin as well as contribute to subsequent regeneration (Lu and Richardson 1993, Perry and Brown 1992, Hu and McLachlan 2002. Recently, it has been recognized that inflammatory responses in the DRG may also contribute to the development pathological pain states (Cui et al, 2000;Xie et al, 2006) through the release of a variety of proinflammatory factors which are capable of sensitizing primary afferent neurons whose cell bodies are housed in the DRG (Taniuchi et al, 1986;Lindholm et al, 1987;Meyer et al, 1992;Hammarberg et al, 1996;Ma & Eisenach, 2002.…”

Section: Cellular Alterations In Satellite Cells Macrophages and Schmentioning

confidence: 99%

An evolving cellular pathology occurs in dorsal root ganglia, peripheral nerve and spinal cord following intravenous administration of paclitaxel in the rat

et al. 2007

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Paclitaxel (Taxol®) is a frontline antineoplastic agent used to treat a variety of solid tumors including breast, ovarian, or lung cancer. The major dose limiting side effect of paclitaxel is a peripheral sensory neuropathy that can last days to a lifetime. To begin to understand the cellular events that contribute to this neuropathy, we examined a marker of cell injury/regeneration (activating transcription factor 3; ATF3), macrophage hyperplasia/hypertrophy; satellite cell hypertrophy in the dorsal root ganglion (DRG) and sciatic nerve as well as astrocyte and microglial activation within the spinal cord at 1, 4, 6 and 10 days following intravenous infusion of therapeutically relevant doses of paclitaxel. At day 1 post-infusion there was an up-regulation of ATF3 in a subpopulation of large and small DRG neurons and this up-regulation was present through day 10. In contrast, hypertrophy of DRG satellite cells, hypertrophy and hyperplasia of CD68+ macrophages in the DRG and sciatic nerve, ATF3 expression in S100β+ Schwann cells and increased expression of the microglial marker (CD11b) and the astrocyte marker glial fibrillary acidic protein in the spinal cord were not observed until day 6 post infusion. The present results demonstrate that using the time points and markers examined, DRG neurons show the first sign of injury which is followed days later by other neuropathological changes in the DRG, peripheral nerve and dorsal horn of the spinal cord. Understanding the cellular changes that generate and maintain this neuropathy may allow the development of mechanism-based therapies to attenuate or block this frequently painful and debilitating condition.

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“…Compared with SCs, activated SCs enhance axonal regeneration in SCI models and significantly promote functional recovery by expressing various neurotrophic factors and cell adhesion molecules and supply the extracellular matrix [33][34][35][36][37] . In this study, after coculture in vitro for 7 d, ASCs promoted the differentiation of MSCs into neuron-like cells that express immature and mature neuron markers, such as β-tubulin 3 and MAP 2.…”

Section: Discussionmentioning

confidence: 99%

“…Injury to the PNS produces a cascade of cellular and molecular events, which promote the proliferation and activation of SCs within the distal nerve stump [24,30] . Activated SCs (ASCs) express various neurotrophic factors and cell adhesion molecules and supply the extracellular matrix, which plays an important role in remyelination and interactions between SCs and axons [31][32][33][34][35] . Compared with SCs, ASCs enhance axonal regeneration in SCI models and significantly promote functional recovery [36,37] .…”

Section: Introductionmentioning

confidence: 99%

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

Yang

Zhang

et al. 2017

Acta Pharmacol Sin

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The adult mammalian CNS has a limited capacity to regenerate after traumatic injury. In this study, a combinatorial strategy to promote axonal regeneration and functional recovery after spinal cord injury (SCI) was evaluated in adult rats. The rats were subjected to a complete transection in the thoracic spinal cord, and multichannel scaffolds seeded with activated Schwann cells (ASCs) and/or rat bone marrow-derived mesenchymal stem cells (MSCs) were acutely grafted into the 3-mm-wide transection gap. At 4 weeks posttransplantation and thereafter, the rats receiving scaffolds seeded with ASCs and MSCs exhibited significant recovery of nerve function as shown by the Basso, Beattie and Bresnahan (BBB) score and electrophysiological test results. Immunohistochemical analyses at 4 and 8 weeks after transplantation revealed that the implanted MSCs at the lesion/graft site survived and differentiated into neuron-like cells and co-localized with host neurons. Robust bundles of regenerated fibers were identified in the lesion/graft site in the ASC and MSC co-transplantation rats, and neurofilament 200 (NF) staining confirmed that these fibers were axons. Furthermore, myelin basic protein (MBP)-positive myelin sheaths were also identified at the lesion/graft site and confirmed via electron microscopy. In addition to expressing mature neuronal markers, sparse MSC-derived neuron-like cells expressed choline acetyltransferase (ChAT) at the injury site of the ASC and MSC co-transplantation rats. These findings suggest that co-transplantation of ASCs and MSCs in a multichannel polymer scaffold may represent a novel combinatorial strategy for the treatment of spinal cord injury.

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GDNF mRNA in Schwann cells and DRG satellite cells after chronic sciatic nerve injury

Cited by 186 publications

References 0 publications

Neurturin and Glial Cell Line-Derived Neurotrophic Factor Receptor-β (GDNFR-β), Novel Proteins Related to GDNF and GDNFR-α with Specific Cellular Patterns of Expression Suggesting Roles in the Developing and Adult Nervous System and in Peripheral Organs

Neurturin and Glial Cell Line-Derived Neurotrophic Factor Receptor-β (GDNFR-β), Novel Proteins Related to GDNF and GDNFR-α with Specific Cellular Patterns of Expression Suggesting Roles in the Developing and Adult Nervous System and in Peripheral Organs

An evolving cellular pathology occurs in dorsal root ganglia, peripheral nerve and spinal cord following intravenous administration of paclitaxel in the rat

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

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