Gel Filtration Chromatography

Kummari, Raghupathi; Bose, Kakoli

doi:10.1007/978-981-16-4987-5_8

Cited by 1 publication

(1 citation statement)

References 35 publications

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“…HSA-conjugated with ZW800-PEG (HSA-ZW800-PEG) was prepared by adding ZW800-PEG NHS ester in the nucleophilic HSA in PBS, pH 8.0 ( Figure 1 b). The reaction mixture was stirred at room temperature for 3 h, followed by purification using gel-filtration chromatography (GFC) connected with Bio-Scale Mini Bio-Gel P-6 Desalting columns (Bio-Rad, Hercules, CA, USA) [ 39 , 40 , 41 ]. HSA-ZW800-PEG shows a clean, distinct peak around 4.2 min, while ZW800-PEG appears at 9.2 min, confirmed by a photodiode array (PDA) detector at 254, 660, and 760 nm wavelengths ( Figure 1 c).…”

Section: Resultsmentioning

confidence: 99%

HSA-ZW800-PEG for Enhanced Optophysical Stability and Tumor Targeting

Jang,

Ser,

Cardenas

et al. 2023

IJMS

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Small molecule fluorophores often face challenges such as short blood half-life, limited physicochemical and optical stability, and poor pharmacokinetics. To overcome these limitations, we conjugated the zwitterionic near-infrared fluorophore ZW800-PEG to human serum albumin (HSA), creating HSA-ZW800-PEG. This conjugation notably improves chemical, physical, and optical stability under physiological conditions, addressing issues commonly encountered with small molecules in biological applications. Additionally, the high molecular weight and extinction coefficient of HSA-ZW800-PEG enhances biodistribution and tumor targeting through the enhanced permeability and retention effect. The unique distribution and elimination dynamics, along with the significantly extended blood half-life of HSA-ZW800-PEG, contribute to improved tumor targetability in both subcutaneous and orthotopic xenograft tumor-bearing animal models. This modification not only influences the pharmacokinetic profile, affecting retention time and clearance patterns, but also enhances bioavailability for targeting tissues. Our study guides further development and optimization of targeted imaging agents and drug-delivery systems.

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Section: Resultsmentioning

confidence: 99%