Gelatin-Based Hydrogels Blended with Gellan as an Injectable Wound Dressing

Abstract

Injectable scaffolds are of great interests for skin regeneration because they can fill irregularly shaped defects through minimally invasive surgical treatments. In this study, an injectable hydrogel from biopolymers is developed and its application as wound dressings is examined. Gelatin-based hydrogels were successfully prepared at body temperature upon blending with low content of gellan, and the synergetic effect on the gel formation was carefully characterized through rheological methods. The electrostatic complexation between gelatin and gellan was confirmed to contribute a continuous hydrogel network. The obtained blend hydrogel demonstrates remarkable shear-thinning and self-recovering properties. For antibacterial purpose, tannic acid was incorporated into the blend hydrogel. In addition, tannic acid-loaded blend hydrogel was verified to accelerate the wound healing on the mice model, significantly than the control groups. Thus, this paper presents a facile approach without chemical modification to construct injectable gelatin-based hydrogels, which have great potential as a wound dressing or tissue scaffold at body temperature.

Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

“…Examples are alginate, chitosan, heparin, chondroitin, proteoglycans, collagen, gelatin, fibrin, keratin, and silk fibroin [18]. The use of alginate and gelatin in tissue engineering, drug delivery, and wound dressings [11,[19][20][21][22][23][24] is very well documented in the literature. If formulated with AgNPs, a hydrophilic environment is required to facilitate the release of the nanoparticles from the polymeric network, maintaining the wound hydrated to promote better healing.…”

Recent Advances in Nanomaterial-Based Wound-Healing Therapeutics

“…The new materials are capable of not only preserving the wound environment but also of transferring active compounds to aid in the wound-healing process [14]. In this regard, various wound-dressing products, such as antibacterial creams, ointments, hydrogels, and antibacterial agents in combination with polymers, are currently available and consist mainly of biodegradable materials such as chitosan, hyaluronic acid, collagen, silicon, cellulose, and gelatin [27][28][29][30][31][32].…”

Injectable hydrogels for tendon and ligament tissue engineering

“…Natural biomaterials, including collagen, fibrin, gelatin, CS, alginate, and HA, have been used in injectable hydrogel preparations. Their advantages and disadvantages are shown in Table 1 (Credi et al, 2014; Di Martino, Sittinger, & Risbud, 2005; Drury & Mooney, 2003; Lee, Singla, & Lee, 2001; Li et al, 2014; Mredha et al, 2017; Palmer, Abreu, Mastrangelo, & Murray, 2009; Pérez, Panitch, & Chmielewski, 2011; Ramshaw, 2016; Sadeghi‐Avalshahr, Nokhasteh, Molavi, Khorsand‐Ghayeni, & Mahdavi‐Shahri, 2017; Shariatinia & Jalali, 2018; Tammi, Day, & Turley, 2002; Tan, Chu, Payne, & Marra, 2009; Zheng et al, 2018).…”

“…Gelatin extracted from collagen has high abundance, low cost, and good biocompatibility, biodegradability, and antigenicity (Zheng et al, 2018). Gelatin methacryloyl (GelMA) hydrogels have some basic properties similar to those of natural ECM due to the presence of cell attachment and matrix metalloproteinase‐reactive peptide motifs, allowing cells to proliferate and spread in GelMA‐based scaffolds (Yue et al, 2015).…”