Gelatin modified with alkoxysilanes (GelmSi) forms hybrid hydrogels for bioengineering applications

Simon, Matthieu; Maumus, Marie; Legrand, Baptiste; Sole, Lilian; Dufaud, Marjorie; Mehdi, Ahmad; Jørgensen, Christian; Noël, Danièle; Subra, Gilles

doi:10.1016/j.bioadv.2023.213321

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…87 Simon et al developed a novel hydrogel precursor, "GelmSi", by modifying gelatin, expanding the range of applicable conditions. 88 Additionally, Liu et al engineered GelMA composite hydrogels with nanoattapulgite, significantly enhancing mechanical strength and promoting bone regeneration and angiogenesis. 89 Fazal et al demonstrated the capability of additive-lathe printing with GelMA hydrogel filaments for producing uniform wall thickness grafts with anisotropic properties.…”

Section: Nanomaterials-based Hybrid Bioinksmentioning

confidence: 99%

“…Chen et al created a biocompatible photosensitive hydrogel material composed of GelMA, suitable for robot-assisted in situ bioprinting of skin wounds . Simon et al developed a novel hydrogel precursor, “GelmSi”, by modifying gelatin, expanding the range of applicable conditions . Additionally, Liu et al engineered GelMA composite hydrogels with nanoattapulgite, significantly enhancing mechanical strength and promoting bone regeneration and angiogenesis .…”

Section: Nanomaterials-based Hybrid Bioinksmentioning

confidence: 99%

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Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine

Chandra,

Reis,

Kundu

et al. 2024

ACS Biomater. Sci. Eng.

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3D bioprinting is recognized as the ultimate additive biomanufacturing technology in tissue engineering and regeneration, augmented with intelligent bioinks and bioprinters to construct tissues or organs, thereby eliminating the stipulation for artificial organs. For 3D bioprinting of soft tissues, such as kidneys, hearts, and other human body parts, formulations of bioink with enhanced bioinspired rheological and mechanical properties were essential. Nanomaterials-based hybrid bioinks have the potential to overcome the above-mentioned problem and require much attention among researchers. Natural and synthetic nanomaterials such as carbon nanotubes, graphene oxides, titanium oxides, nanosilicates, nanoclay, nanocellulose, etc. and their blended have been used in various 3D bioprinters as bioinks and benefitted enhanced bioprintability, biocompatibility, and biodegradability. A limited number of articles were published, and the above-mentioned requirement pushed us to write this review. We reviewed, explored, and discussed the nanomaterials and nanocomposite-based hybrid bioinks for the 3D bioprinting technology, 3D bioprinters properties, natural, synthetic, and nanomaterial-based hybrid bioinks, including applications with challenges, limitations, ethical considerations, potential solution for future perspective, and technological advancement of efficient and cost-effective 3D bioprinting methods in tissue regeneration and healthcare.

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Section: Nanomaterials-based Hybrid Bioinksmentioning

confidence: 99%

Section: Nanomaterials-based Hybrid Bioinksmentioning

confidence: 99%

Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine

Chandra,

Reis,

Kundu

et al. 2024

ACS Biomater. Sci. Eng.

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“…Then, gelatin was silylated with functionalization degree of 40% or 100% (Gelatin-PTES-40 and Gelatin-PTES-100) using an already described protocol [30]. Briefly, Gelatin-PTES were obtained by adding ICPTES (5 eq or 0.5 eq for Gelatin-PTES-40 and Gelatin-PTES-100 respectively) and N, N-Diisopropylethylamine (DIEA, 10 eq) to 30 ml of a 2% (w/ v) gelatin solution.…”

Section: Synthesis Of Tetra-triethoxysilane Functionalized Pla (Pla-4...mentioning

confidence: 99%

Development of hybrid bioactive nanofibers composed of star Poly(lactic acid) and gelatin by sol–gel crosslinking during the electrospinning process

Belabbes,

Simon,

Leon-Valdivieso

et al. 2023

Nanotechnology

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The design of a biomimetic scaffold is a major challenge in tissue engineering to promote tissue reconstruction. The use of synthetic polymer nanofibers is widely described as they provide biocompatible matrices whose topography mimics natural extracellular matrix (ECM). To closely match the biochemical composition of the ECM, bioactive molecules such as gelatin are added to the nanofibers to enhance cell adhesion and proliferation. To overcome the rapid solubilization of gelatin in biological fluids and to allow a lasting biological effect, the covalent crosslinking of this macromolecule in the network is crucial. The sol–gel route offers the possibility of gentle crosslinking during shaping but is rarely combined with electrospinning. In this study, we present the creation of Poly(lactic acid)/Gelatin hybrid nanofibers by sol–gel route during electrospinning. To enable sol–gel crosslinking, we synthesized star-shaped PLA and functionalized it with silane groups; then we functionalized gelatin with the same groups for their subsequent reaction with the polymer and thus the creation of the hybrid nanonetwork. We evaluated the impact of the presence of gelatin in Poly(lactic acid)/Gelatin hybrid nanofibers at different percentages on the mechanical properties, nanonetwork crosslinking, degradation and biological properties of the hybrid nanofibers. The addition of gelatin modulated nanonetwork crosslinking that impacted the stiffness of the nanofibers, resulting in softer materials for the cells. Moreover, these hybrid nanofibers also showed a significant improvement in fibroblast proliferation and present a degradation rate suitable for tissue reconstruction. Finally, the bioactive hybrid nanofibers possess versatile properties, interesting for various potential applications in tissue reconstruction.

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“…[ 6,7 ] Compounding with this issue, as Geltrex's components are unknown, other soluble factors cannot be added or removed to adjust the matrix to support the growth of different tissue types. [ 8 ] Besides, reports have also showed that Geltrex may be contaminated with lactate dehydrogenase‐elevating virus (LDHV), a naturally occurring virus in mice, [ 9 ] which can affect the immune system and inhibit tumor growth. [ 10 ]…”

Section: Introductionmentioning

confidence: 99%

Injectable Hydrogels Prepared Using Novel Synthetic Short Peptides with Defined Structure and Gelatin as Scaffolds to Support Cell and Tissue Growth

Lee,

Balakrishnan,

et al. 2023

Adv Healthcare Materials

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Animal‐derived basement‐membrane matrices such as Geltrex are used to grow cells and tissues. Particularly, these are commonly applied to support tumor growth in animals for cancer research. However, a material derived from an animal source has an undefined composition, and may thus have unavoidable batch‐to‐batch variation in properties. To overcome these issues, a series of synthetic short peptides to form hydrogels is designed in combination with gelatin to promote cell adhesion and growth. The peptides have sequences of (X1Y1X2Y2)2, where X1 and X2 are hydrophobic residues, while Y1 and Y2 are hydrophilic residues. The peptides spontaneously fold and self‐assemble into a β‐sheet secondary structure upon contact with salts, and then aggregate to form hydrophilic networks of hydrogels. Hybrid hydrogels formed by mixing the peptide IEVEIRVK (IVK8) with gelatin are injectable and enzymatically degradable. The hybrid hydrogels at optimal compositions support SW480 and HepG2 tumor spheroid growth in vitro as effectively as Geltrex. More importantly, the peptide/gelatin hydrogels support tumor growth in a SW480 human colorectal adenocarcinoma xenograft mouse model. Altogether, the results illustrate that the synthetic peptide/gelatin hybrid hydrogel is a promising scaffold that can be used to support cell and tissue growth both in vitro and in vivo.

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Gelatin modified with alkoxysilanes (GelmSi) forms hybrid hydrogels for bioengineering applications

Cited by 5 publications

References 34 publications

Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine

Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine

Development of hybrid bioactive nanofibers composed of star Poly(lactic acid) and gelatin by sol–gel crosslinking during the electrospinning process

Injectable Hydrogels Prepared Using Novel Synthetic Short Peptides with Defined Structure and Gelatin as Scaffolds to Support Cell and Tissue Growth

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