Gellan gum-based hydrogels support the recreation of the dermal papilla microenvironment

Abreu, Carla M.; Lago, Manuela E.L.; Pires, Joana; Reis, Rui L.; Silva, Lucília P. da; Marques, Alexandra P.

doi:10.1016/j.bioadv.2023.213437

Cited by 2 publications

(2 citation statements)

References 58 publications

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“… [ [40] , [41] , [42] , [43] , [44] , [45] ] Biofunctionalization Can be functionalized with bioactive molecules. Tailors hydrogel for specific cellular responses Direct biofunctionalization of synthetic polymers like Poly (lactic-co-glycolic acid) compared to naturally derived polymers like gellan gum, silk fibroin, and chitosan may present challenges [ 34 , 46 ] Sterilizability Compatible with various sterilization methods. Ensures safety and sterility for clinical use Synthetic polymers generally maintain their structural integrity, whereas natural polymers such as silk fibroin, sodium alginate, and chitosan may undergo degradation when subjected to certain sterilization techniques.…”

Section: Introductionmentioning

confidence: 99%

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Exploring gellan gum-based hydrogels for regenerating human embryonic stem cells in age-related macular degeneration therapy: A literature review

Moyo,

Adali,

Tulay

2024

Regenerative Therapy

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Section: Introductionmentioning

confidence: 99%

“…Synthetic polymers like Polyethylene Glycol exhibit variability in stability. [ [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] ] …”

Section: Introductionmentioning

confidence: 99%

Exploring gellan gum-based hydrogels for regenerating human embryonic stem cells in age-related macular degeneration therapy: A literature review

Moyo,

Adali,

Tulay

2024

Regenerative Therapy

View full text Add to dashboard Cite

3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

Paoletti,

Baschieri,

Migliorini

et al. 2024

J Biomedical Materials Res

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The ever‐growing need for new tissue and organ replacement approaches paved the way for tissue engineering. Successful tissue regeneration requires an appropriate scaffold, which allows cell adhesion and provides mechanical support during tissue repair. In this light, an interpenetrating polymer network (IPN) system based on biocompatible polysaccharides, dextran (Dex) and gellan (Ge), was designed and proposed as a surface that facilitates cell adhesion in tissue engineering applications. The new matrix was developed in glycerol, an unconventional solvent, before the chemical functionalization of the polymer backbone, which provides the system with enhanced properties, such as increased stiffness and bioadhesiveness. Dex was modified introducing methacrylic groups, which are known to be sensitive to UV light. At the same time, Ge was functionalized with RGD moieties, known as promoters for cell adhesion. The printability of the systems was evaluated by exploiting the ability of glycerol to act as a co‐initiator in the process, speeding up the kinetics of crosslinking. Following semi‐IPNs formation, the solvent was removed by extensive solvent exchange with HEPES and CaCl2, leading to conversion into IPNs due to the ionic gelation of Ge chains. Mechanical properties were investigated and IPNs ability to promote osteoblasts adhesion was evaluated on thin‐layer, 3D‐printed disk films. Our results show a significant increase in adhesion on hydrogels decorated with RGD moieties, where osteoblasts adopted the spindle‐shaped morphology typical of adherent mesenchymal cells. Our findings support the use of RGD‐decorated Ge/Dex IPNs as new matrices able to support and facilitate cell adhesion in the perspective of bone tissue regeneration.

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Gellan gum-based hydrogels support the recreation of the dermal papilla microenvironment

Cited by 2 publications

References 58 publications

Exploring gellan gum-based hydrogels for regenerating human embryonic stem cells in age-related macular degeneration therapy: A literature review

Exploring gellan gum-based hydrogels for regenerating human embryonic stem cells in age-related macular degeneration therapy: A literature review

3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

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