Gender differences in the bronchoalveolar lavage cell proteome of patients with chronic obstructive pulmonary disease

Kohler, Maxie; Sandberg, AnnSofi; Kjellqvist, Sanela; Thomas, Andreas; Karimi, Reza; Nyrén, Sven; Eklúnd, Anders; Thevis, Mario; Sköld, C. Magnus; Wheelock, Åsa M.

doi:10.1016/j.jaci.2012.09.024

Cited by 65 publications

(84 citation statements)

References 53 publications

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“…We also investigated the gene expression profile of bronchial epithelial cells in bronchial brush biopsies harvested from healthy subjects by selecting data of interest from a broad mRNA analyses of the COSMIC cohort material (22,23). Here, we found that these bronchial epithelial cells contain mRNA for IL26, TLR3, TLR7 and TLR8 among others ( Figure 6A), which are compatible with our finding on primary bronchial epithelial cells in vitro.…”

Section: Interleukin-26 and Its Archetype Signaling Responses In Bronsupporting

confidence: 85%

“…The data (raw signals) were presented as relative fluorescence units (RFU). Here, 10 randomly selected bronchial brush biopsy samples from the human (4 male and 6 female) donors were selected from the pool of the COSMIC cohort (22,23). The analyses of mRNA were then performed on the cells in the bronchial brush biopsy samples as previously described (27).…”

Section: Cell Culture and Stimulationmentioning

confidence: 99%

“…Thus, data and samples from 37 control subjects in the Karolinska Institutet COSMIC cohort (ClinicalTrials.gov Identifier: NCT02627872) were utilized and some of the clinical data sets have been utilized in other studies (22,23). The COSMIC cohort study was previously approved by the Regional committee for ethical review in Stockholm, Sweden (Diary No 2006/ 959-31/1).…”

Section: Bronchoscopy and Collection Of Bronchial Biopsies Bronchoalmentioning

confidence: 99%

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Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

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Kaarteenaho

Lappi‐Blanco

et al. 2017

Mol Med

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Interleukin (IL)-26 is abundant in human airways and this cytokine is involved in the local immune response to a bacterial stimulus in vivo. Specifically, local exposure to the toll-like receptor (TLR) 4 agonist endotoxin does increase IL-26 in human airways and this cytokine potentiates chemotactic responses in human neutrophils. In addition to T-helper (Th) 17 cells, alveolar macrophages can produce IL-26, but it remains unknown whether this cytokine can also be produced in the airway mucosa per se in response to a viral stimulus. Here, we evaluated whether this is the case using primary bronchial epithelial cells from the airway epithelium in vitro and explored the signaling mechanisms involved, including the modulatory effects of additional Th17 cytokines. Finally, we assessed IL-26 and its archetype signaling responses in healthy human airways in vivo. We found increased transcription and release of IL-26 protein after stimulation with the viral-related double stranded (ds) RNA polyinosinic-polycytidylic acid (poly-IC) and showed that this IL-26 release involved mitogen-activated protein (MAP) kinases and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). The release of IL-26 in response to a viral stimulus was modulated by additional Th17 cytokines. Moreover, there was transcription of IL26 mRNA and expression of the protein in epithelial cells of bronchial brush and tissue biopsies respectively after harvest in vivo. In addition, the extracellular IL-26 protein concentrations in bronchoalveolar lavage (BAL) samples did correlate with increased epithelial cell transcription of an archetype intracellular signaling molecule downstream of the IL-26-receptor complex, STAT1, in the bronchial brush biopsies. Thus, our study suggests that viral stimulation causes the production of IL-26 in lining epithelial cells of human airways, structural cells that constitute a critical immune barrier and that this production is modulated by Th17 cytokines.

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Section: Interleukin-26 and Its Archetype Signaling Responses In Bronsupporting

confidence: 85%

Section: Cell Culture and Stimulationmentioning

confidence: 99%

Section: Bronchoscopy and Collection Of Bronchial Biopsies Bronchoalmentioning

confidence: 99%

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Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Fru

Kaarteenaho

Lappi‐Blanco

et al. 2017

Mol Med

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“…As a control group, healthy age-matched never smokers and current smokers with normal lung function from the Karolinska COPD and Smoking from an OMIC Perspective (COSMIC) cohort was used16 consisting of 79 healthy volunteers who underwent bronchoscopy and BAL. Another 15 individuals served as a control group for comparison of biopsies.…”

Section: Methodsmentioning

confidence: 99%

Signs of immune activation and local inflammation are present in the bronchial tissue of patients with untreated early rheumatoid arthritis

et al. 2015

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“…Improvements to the two-dimensional gel electrophoresis in the form of differential protein labelling with multiple dyes has recently emphasised the utility of the two-dimensional gel technique for finding biomarkers of lung diseases in challenging biofluids such as sputum [69]. KOHLER et al [70] recently utililised multiplexed differential gel electrophoresis to identify a female-dominated subphenotype of COPD. In this study, a subset of 19 proteins in alveolar macrophages primarily originating from the lysosomal activity and oxidative phosphorylation pathways were found to provide classification of female COPD patients with 78% predictive power.…”

Section: Proteomicsmentioning

confidence: 99%

Application of ’omics technologies to biomarker discovery in inflammatory lung diseases

et al. 2013

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Inflammatory lung diseases are highly complex in respect of pathogenesis and relationships between inflammation, clinical disease and response to treatment. Sophisticated large-scale analytical methods to quantify gene expression (transcriptomics), proteins (proteomics), lipids (lipidomics) and metabolites (metabolomics) in the lungs, blood and urine are now available to identify biomarkers that define disease in terms of combined clinical, physiological and patho-biological abnormalities. The aspiration is that these approaches will improve diagnosis, i.e. define pathological phenotypes, and facilitate the monitoring of disease and therapy, and also, unravel underlying molecular pathways. Biomarker studies can either select predefined biomarker(s) measured by specific methods or apply an ''unbiased'' approach involving detection platforms that are indiscriminate in focus. This article reviews the technologies presently available to study biomarkers of lung disease within the 'omics field. The contributions of the individual 'omics analytical platforms to the field of respiratory diseases are summarised, with the goal of providing background on their respective abilities to contribute to systems medicine-based studies of lung disease. @ERSpublications Summary of the application of 'omics-based analytical platforms for biomarker discovery in inflammatory lung diseases

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Gender differences in the bronchoalveolar lavage cell proteome of patients with chronic obstructive pulmonary disease

Cited by 65 publications

References 53 publications

Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Interleukin-26 Production in Human Primary Bronchial Epithelial Cells in Response to Viral Stimulation: Modulation by Th17 cytokines

Signs of immune activation and local inflammation are present in the bronchial tissue of patients with untreated early rheumatoid arthritis

Application of ’omics technologies to biomarker discovery in inflammatory lung diseases

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