Gender related differences in the oxidative stress response to PCB exposure in an endangered goodeid fish (Girardinichthys viviparus)

Vega-López, Armando; Galár-Martínez, Marcela; Jiménez-Orozco, Fausto Alejandro; Garcı́a-Latorre, Ethel; Domínguez-López, María Lilia

doi:10.1016/j.cbpa.2006.04.022

Cited by 53 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…XRE is usually found in the promoter region of xenobiotics-metabolizing battery genes, such as cytochrome P450 (Ryu et al, 1996), glutathione S-transferase (Pimental et al, 1993), and UDP-glucuronosyltransferase 1A1 (Yueh et al, 2003), as well as mammalian sod1 (Yoo et al, 1999a;Park and Rho, 2002). The identification of XRE in this study is also in agreement with previous observations on the differential modulation of sod1 in other fish species exposed to xenobiotic compounds (Pedrajas et al, 1998;Shi et al, 2005;Vega-López et al, 2007). However unlike murine sod1 (Yoo et al, 1999b), the hmsod1 did not possess a metal-responsive element (MRE) in its promoter region, although heavy metal exposure could stimulate sod1 transcription in this species as well (see Cho et al, 2009b).…”

Section: Characteristics Of the 5'-flanking Regulatory Regionsupporting

confidence: 92%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

View full text Add to dashboard Cite

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

show abstract

Section: Characteristics Of the 5'-flanking Regulatory Regionsupporting

confidence: 92%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

View full text Add to dashboard Cite

show abstract

“…The presence of the MRE in the Mn-SOD regulatory region may provide support for a previous report of heavy metal-activated Mn-SOD expression in an aquatic animal ( [4]; see also [23]). The presence of the XRE, a core sequence recognized by the aryl hydrocarbon receptor (AhR), in the teleost SOD gene promoter is congruent with previous observations of differential SOD modulation in fish organs during exposure to xenobiotic compounds ( [24,25]; see also [26]). …”

Section: Introductionsupporting

confidence: 76%

Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)

Cho

Lee

Bang

et al. 2009

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Moreover, Wang and colleagues (2010) recently found that the gene expression of Mn-SOD was significantly increased after immunostimulation in red swamp crayfish. Several reports also have evaluated differential SOD modulation in fish organs during exposure to xenobiotic compounds (Shi et al, 2005;Vega-López et al, 2007;An et al, 2008;Cho et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Wang

Osatomi

Nagatomo

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26 kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64 bp 5'-non-coding region, a 675 bp open reading frame encoding 225 amino acids, and a 465 bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs.

show abstract

Gender related differences in the oxidative stress response to PCB exposure in an endangered goodeid fish (Girardinichthys viviparus)

Cited by 53 publications

References 36 publications

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

Contact Info

Product

Resources

About