Gene Banking: The Freezing Strategy

Casas, Isabel; Flores, E.

doi:10.1007/978-3-642-35049-8_11

Cited by 9 publications

(10 citation statements)

References 225 publications

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“…This is a very important asset, especially if one takes into account that sperm cryopreservation has been regarded as being the most efficient method for storing boar sperm samples for a long period of time (Holt, ). However, a greater utilization of this technique has been hampered so far because of its lower fertility rates and litter sizes when compared with extended semen, despite all of the efforts being made in this regard (Almlid & Hofmo, ; Johnson et al ., ; Casas & Flores, ). In this way, our results may be relevant to further develop boar sperm freeze‐thawing protocols that could yield ‘in vivo’ fertility results not far below to those obtained with extended semen.…”

Section: Discussionmentioning

confidence: 99%

Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen‐thawed boar semen

et al. 2013

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SUMMARYThe main aim of this work was to evaluate how supplementing freezing media with reduced glutathione (GSH) affected the 'in vivo' fertilizing ability of boar semen subjected to cryopreservation procedures. With this purpose, 12 ejaculates coming from 12 boars were cryopreserved in the presence or absence of 2 mM GSH, whereas the same number of extended ejaculates coming from the same boars was used as negative/farm controls. Eight different sperm parameters (levels of free-cysteine residues in sperm nucleoproteins, DNA fragmentation, sperm viability, acrosome-membrane integrity, intracellular peroxide and superoxide levels, and total and progressive sperm motility) were evaluated before freezing and after 30 and 240 min of thawing. In addition, a total of 180 multiparous sows were used in the field fertility trials, the females being randomly divided into three groups and inseminated with extended, frozen-thawed control or frozen-thawed semen supplemented with 2 mM GSH. The presence of GSH in the freezing media significantly (p < 0.05) increased farrowing rates and the number of total born piglets and alive born piglets, and partially counteracted the cryopreservation-induced damages inflicted on frozen-thawed spermatozoa. We can thus conclude that supplementing freezing media with 2 mM GSH greatly improves boar sperm cryopreservation technology, as it significantly improves the fertilizing ability of frozen-thawed spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen‐thawed boar semen

et al. 2013

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“…However, it is important to notice that evaluating sperm motility may not be suitable to evaluate the cryopreservation success, as it may not be correlated with fertilizing ability (McNamara and Knox ). For this reason, other parameters, such as integrity and lipid disorder of plasma membrane and acrosome integrity need to be evaluated (Casas and Flores ). Recently, Daigneault et al.…”

Section: Evaluation Of Sperm Quality At Post‐thawingmentioning

confidence: 99%

“…While this method of storage may be useful for planning of AI centres and allows preserving genetic material for decades (Benson et al. ; Casas and Flores ), recent reports have shown that long‐term storage in liquid nitrogen may also affect the post‐thaw sperm quality (Fraser et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…). Such a cryodamage is largely due to the high sensitivity of boar sperm to cold shock and peroxidative damage because of the composition of their plasma membrane, which contents high levels of unsaturated phospholipids and low cholesterol levels (Casas and Flores ). As a result, cooling and freeze–thawing procedures induce sperm membrane changes that lead to its desestabilization, thereby affecting calcium homoeostasis, acrosome integrity and membrane lipid disorder (Maxwell and Johnson ; Leahy and Gadella ; Vadnais and Althouse ).…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

Yeste

2015

Reprod Domestic Animals

112

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ContentsWhile sperm cryopreservation is the best technology to store boar semen for long-term periods, only 1% of all artificial inseminations (AI) conducted worldwide are made using frozen-thawed boar sperm. With the emergence of long-term extenders for liquid storage, the use of cryopreserved sperm in routine AI is less required. However, banks of boar semen contain cryopreserved sperm and planning inseminations in AI centres may benefit from the use of frozen-thawed semen. Therefore, there is an interest in the use of this technology to preserve boar sperm. In this regard, although the first attempts to cryopreserve boar semen date back to the seventies and this technology is still considered as optimal, some relevant improvements have been made in the last decade. After giving a general picture about boar sperm cryodamage, the present review seeks to shed light on these recent cryopreservation advances. These contributions regard to protein markers for predicting ejaculate freezability, sperm selection prior to start cryopreservation procedures, additives to freezing and thawing extenders, relevance of the AI-technique and insemination-toovulation interval. In conclusion, most of these progresses have allowed counteracting better boar sperm cryodamage and are thus considered as forward steps for this storage method. It is also worth noting that, despite being lower than fresh/ extended semen, reproductive performance outcomes following AI with frozen-thawed boar sperm are currently acceptable.

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“…In fact, although sperm cryopreservation is the best method to store spermatozoa for long periods, it is only used in 2% of cases (Roca et al 2011). Among other reasons, this is because sperm survival is reduced following cryopreservation as a result of cryodamage to the sperm membrane and nucleus (Holt 2000;Casas et al 2009;Benson et al 2012;Yeste et al 2013a), which may ultimately result in lower fertility rates and litter sizes (Almlid and Hofmo 1995;Johnson et al 2000;Casas and Flores 2013). In addition, not all ejaculates have the same ability to withstand freeze-thawing and individual differences between ejaculates exist (Thurston et al 2001(Thurston et al , 2002; consequently, ejaculates are classified as 'good freezability ejaculates' (GFE) and 'poor freezability ejaculates' (PFE; Casas et al 2009;Yeste et al 2013b).…”

Section: Introductionmentioning

confidence: 99%

Effects of reduced glutathione on acrosin activity in frozen–thawed boar spermatozoa

Estrada

Rodríguez-Gil

Álamo

et al. 2017

Reprod. Fertil. Dev.

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In pigs, acrosin activity in extended semen is correlated with reproductive performance and has recently been identified as a freezability marker. Reduced glutathione (GSH) is known to decrease sperm cryodamage and increase the reproductive performance of frozen-thawed boar spermatozoa. However, the effects of GSH on the acrosin activity of good and poor freezability ejaculates (GFE and PFE, respectively) is yet to be examined. The present study investigated how supplementing cryopreservation media with GSH affected acrosin activity in GFE and PFE, as well as the relationship between acrosin activity and reproductive performance in frozen-thawed boar spermatozoa. In addition, we examined whether the increase in fertility rates and litter sizes observed after the addition of 2mM GSH to cryopreservation extenders was related to acrosin activity. Supplementing freezing media with 2mM GSH partially counteracted the cryopreservation-related decrease in acrosin activity in GFE but not PFE. Acrosin activity was found to be significantly correlated with in vivo reproductive performance of frozen-thawed boar semen. In conclusion, the effects of adding GSH to freezing extenders on the acrosin activity of frozen-thawed boar spermatozoa rely on the intrinsic freezability of the ejaculate. Furthermore, the maintenance of proper acrosin activity could contribute to the increase in reproductive performance mediated by GSH.

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Gene Banking: The Freezing Strategy

Cited by 9 publications

References 225 publications

Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen‐thawed boar semen

Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen‐thawed boar semen

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

Effects of reduced glutathione on acrosin activity in frozen–thawed boar spermatozoa

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