Gene Cloning and Bioinformatics Analysis of Glycoprotein H Gene of Pseudorabies Virus Strain SL

Abstract

The glycoprotein H(gH) gene homologue of pseudorabies virus wild strain SL(PRV-SL) was cloned by degenerate polymerase chain reaction (PCR) from PRV infected vero cells. Bioinformatics analysis was performed to predict the characteristics of gH, the results indicated that gH gene encoded a polypeptide, molecular mass of 71.95kda and comprising 687 amino acids. The protein had one signal peptide between 1 and 24AA, one transmembrane region 645-667AA, 27 antigenic determinants and the hydrophobicity between -2.117 and 3.178. The prediction of secondary structure showed that gH had consisted highly of alpha helix(Hh) and random coil(Cc). The phylogenetic tree showed that PRV-SL was similar to others of the Alphaherpesvirinae, and that gH had higher conservative in Alphaherpesvirinae. Cloning and analysis of gH gene laid foundation to further research and exploitation.