Gene cluster lock after pheromone receptor gene choice

Roppolo, Daniele; Vollery, Sarah; Kan, Chenda; Lüscher, Christian; Broillet, Marie-Christine; Rodríguez, Iván

doi:10.1038/sj.emboj.7601782

Cited by 61 publications

(63 citation statements)

References 30 publications

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“…It is known that a substantial number of pseudogenes are present in the mammalian OR gene families (Ϸ30% of total OR genes in the mouse and Ϸ60% in human are nonfunctional). If an activated LCR has selected a pseudogene and has been trapped by its promoter, other LCRs must undergo a similar process to ensure the activation of an intact OR gene (32). Pseudogene may help slow down the process of OR gene activation and further reduce the frequency of the simultaneous activation of two different OR genes.…”

Section: Extent Of the Effect Of H Region Activitymentioning

confidence: 99%

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

Nishizumi

Inoue

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

108

118

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We have previously reported that a 2.1-kb homology (H) sequence, conserved between mouse and human, regulates the odorant receptor (OR) gene MOR28 in transgenic mice. Here, we narrowed down the essential sequences of the H to a core of 124 bp by using a transient expression system in zebrafish embryos. Transgenic experiments in mice demonstrated that the core-H sequence is sufficient to endow expression of the MOR28 minigene. Deletion and mutation analyses of the core-H region revealed two homeodomain sequences to be essential for the H enhancer activity. Targeted deletion of the core-H abolished expression of three proximal OR genes, MOR28, MOR10, and MOR83, in cis, indicating the presence of another locus control region/enhancer in the downstream region, that regulates four distal OR genes in the same MOR28 cluster. In the heterozygous mice, the H ؊ phenotype of the mutant allele was not rescued by the wild-type H ؉ allele in trans.olfactory sensory neuron ͉ gene targeting ͉ locus control region ͉ transgenic mouse ͉ zebrafish

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Section: Extent Of the Effect Of H Region Activitymentioning

confidence: 99%

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

Nishizumi

Inoue

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

108

118

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“…In situ hybridizations were performed as described previously (Roppolo et al, 2007). Briefly, tissues were embedded in OCT and 14 m cryostat sections were mounted on Superfrost Plus slides.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

The wiring of Grueneberg ganglion axons is dependent on neuropilin 1

et al. 2012

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SUMMARYThe Grueneberg ganglion is a specialized olfactory sensor. In mice, its activation induces freezing behavior. The topographical map corresponding to the central projections of its sensory axons is poorly defined, as well as the guidance molecules involved in its establishment. We took a transgenic approach to label exclusively Grueneberg sensory neurons and their axonal projections. We observed that a stereotyped convergence map in a series of coalescent neuropil-rich structures is already present at birth. These structures are part of a peculiar and complex neuronal circuit, composed of a chain of glomeruli organized in a necklace pattern that entirely surrounds the trunk of the olfactory bulb. We found that the necklace chain is composed of two different sets of glomeruli: one exclusively innervated by Grueneberg ganglion neurons, the other by axonal inputs from the main olfactory neuroepithelium. Combining the transgenic Grueneberg reporter mouse with a conditional null genetic approach, we then show that the axonal wiring of Grueneberg neurons is dependent on neuropilin 1 expression. Neuropilin 1-deficient Grueneberg axonal projections lose their strict and characteristic avoidance of vomeronasal glomeruli, glomeruli that are innervated by secondary neurons expressing the repulsive guidance cue and main neuropilin 1 ligand Sema3a. Taken together, our observations represent a first step in the understanding of the circuitry and the coding strategy used by the Grueneberg system.

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“…G protein-coupled vomeronasal chemoreceptor proteins are encoded by 3 unrelated multigene families-the V1r (Dulac and Axel 1995), V2r (Herrada and Dulac 1997;Matsunami and Buck 1997;Ryba and Tirindelli 1997), and Fpr-rs (Liberles et al 2009;Rivière et al 2009) genes. Monogenic receptor expression and type-specific coexpression of G protein α-subunits (G αi2 /G αo ) confer a distinct receptor "identity" to each VSN (Roppolo et al 2007) and result in topographical segregation into at least 2 VSN subpopulations.…”

Section: Introductionmentioning

confidence: 99%

Elevated Cytosolic Cl⁻Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Untiet¹,

Moeller²,

Ibarra-Soria³

et al. 2016

CHEMSE

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In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component-a Ca 2+ -activated Cl − current. As the physiological role of such a current is a direct function of the Cl − equilibrium potential, we determined the intracellular Cl − concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl − -sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl − levels in dendritic knobs, a substantially lower Cl − concentration in vomeronasal sustentacular cells, and an apparent Cl − gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca 2+ -activated Cl − channels.

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Gene cluster lock after pheromone receptor gene choice

Cited by 61 publications

References 30 publications

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

The wiring of Grueneberg ganglion axons is dependent on neuropilin 1

Elevated Cytosolic Cl⁻Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

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Gene cluster lock after pheromone receptor gene choice

Cited by 61 publications

References 30 publications

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

Deletion of the core- H region in mice abolishes the expression of three proximal odorant receptor genes in cis

The wiring of Grueneberg ganglion axons is dependent on neuropilin 1

Elevated Cytosolic Cl−Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

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Elevated Cytosolic Cl⁻Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons