Gene Expression Analysis of Peripheral Blood Cells Reveals Toll-Like Receptor Pathway Deregulation in Colorectal Cancer

Xu, Ye; Xu, Qinghua; Li, Yang; Liu, Fang; Ye, Xun; Wu, Fei; Ni, Shujuan; Tan, Cong; Cai, Guoxiang; Xia, Meng; Cai, Sanjun; Du, Xiang

doi:10.1371/journal.pone.0062870

Cited by 15 publications

(13 citation statements)

References 47 publications

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“…SLE is the prototypic systemic autoimmune disease and possesses a gender-dependent incidence characterized by a male/female ratio of 1 : 9 [ 65 , 66 ]. Although our above described results indicated a gender-associated gene difference of B cells from both healthy subjects and SLE patients between female and male, it is very difficult to figure out the specific genes responsible for disruption of immune balance of B cells in SLE patients.…”

Section: Discussionmentioning

confidence: 99%

Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen

Fan

Dong

Zhao

et al. 2014

Journal of Immunology Research

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The aim of the present study was to investigate mechanism of the gender differences of B cells. The results showed that 358 differential gene expressions (DEGs) were displayed between healthy females and males. Compared with male, 226 and 132 genes were found to be up- and downregulated in the female. 116 genes displayed possible correlation with estrogen. Moreover, the upregulated DEGs (Cav1, CD200R1, TNFRSF17, and CXCR3) and downregulated DEGs (EIF1AY and DDX3Y) in healthy female may be involved in gender predominance of some immune diseases. Furthermore, signaling pathway analysis for estrogen-relevant DEGs showed that only 26 genes were downregulated in SLE female versus SLE male, of which expressions of 8 genes had significant difference between SLE females and SLE males but are having nonsignificant difference between healthy females and healthy males. Except for the 5 Y-chromosome-related genes or varients, only 3 DEGs (LTF, CAMP, and DEFA4) were selected and qRT-PCR confirmed that the expressions of LTF and CAMP decreased significantly in B cells from female SLE patients. These data indicated that the gender differences were existent in global gene expression of B cells and the difference may be related to estrogen.

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Section: Discussionmentioning

confidence: 99%

Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen

Fan

Dong

Zhao

et al. 2014

Journal of Immunology Research

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“…Evidence of increased B cell activation in colon cancer patients exists via increased Toll-like receptor (TLR) signaling in peripheral B cells [ 69 ] and tumor-specific antibody production [ 70 ] as seen in breast cancer. Mouse models of colon cancer demonstrate the capacity for TLS to form.…”

Section: Introductionmentioning

confidence: 99%

Role of Tertiary Lymphoid Structures (TLS) in Anti-Tumor Immunity: Potential Tumor-Induced Cytokines/Chemokines that Regulate TLS Formation in Epithelial-Derived Cancers

Pimenta

Barnes

2014

Cancers

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Following the successes of monoclonal antibody immunotherapies (trastuzumab (Herceptin®) and rituximab (Rituxan®)) and the first approved cancer vaccine, Provenge® (sipuleucel-T), investigations into the immune system and how it can be modified by a tumor has become an exciting and promising new field of cancer research. Dozens of clinical trials for new antibodies, cancer and adjuvant vaccines, and autologous T and dendritic cell transfers are ongoing in hopes of identifying ways to re-awaken the immune system and force an anti-tumor response. To date, however, few consistent, reproducible, or clinically-relevant effects have been shown using vaccine or autologous cell transfers due in part to the fact that the immunosuppressive mechanisms of the tumor have not been overcome. Much of the research focus has been on re-activating or priming cytotoxic T cells to recognize tumor, in some cases completely disregarding the potential roles that B cells play in immune surveillance or how a solid tumor should be treated to maximize immunogenicity. Here, we will summarize what is currently known about the induction or evasion of humoral immunity via tumor-induced cytokine/chemokine expression and how formation of tertiary lymphoid structures (TLS) within the tumor microenvironment may be used to enhance immunotherapy response.

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“…[ 22 ] List 3 of 2,272 genes was identified in peripheral blood mononuclear cells samples. [ 25 ] Then, we matched the 3 bioinformatics gene lists with our list of genes expressed above signal cut-off experimentally generated by the GeneChip, using the Venny tool. [ 26 ] Genes shared between the Affymetrix chip arrays and the described lists were used for further gene selection ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Feasibility of Unbiased RNA Profiling of Colorectal Tumors: A Proof of Principle

Moshayoff¹,

Faktor²,

Laghi³

et al. 2016

PLoS ONE

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Despite recent advances in molecular profiling of colorectal cancer (CRC), as of yet this has not translated into an unbiased molecular liquid biopsy profile which can accurately screen for early CRC. In this study we depict the profile of early stage CRC as well as for advanced adenomas (AA) by combination of current molecular knowledge with microarray technology, using efficient circulating free plasma RNA purification from blood and RNA amplification technologies. We joined literature search with Affymetrix gene chip experimental procedure to draw the circulating free plasma RNA profile of colorectal cancer disease reflected in blood. The RNA panel was tested by two datasets comparing patients with CRC with healthy subjects and patients with AA to healthy subjects. For the CRC patient cohort (28 CRC cases vs. 41 healthy controls), the ROC analysis of the selected biomarker panel generated a sensitivity of 75% and a specificity of 93% for the detection of CRC using 8-gene classification model. For the AA patient cohort (28 subjects vs. 46 healthy controls), a sensitivity of 60% and a specificity of 87% were calculated using a 2-gene classification model. We have identified a panel of 8 plasma RNA markers as a preliminary panel for CRC detection and subset markers suitable for AA detection. Subjected to extensive clinical validation we suggest that this panel represents a feasible approach and a potential strategy for noninvasive early diagnosis, as a first-line screening test for asymptomatic, average-risk population before colonoscopy.

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Gene Expression Analysis of Peripheral Blood Cells Reveals Toll-Like Receptor Pathway Deregulation in Colorectal Cancer

Cited by 15 publications

References 47 publications

Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen

Gender Differences of B Cell Signature in Healthy Subjects Underlie Disparities in Incidence and Course of SLE Related to Estrogen

Role of Tertiary Lymphoid Structures (TLS) in Anti-Tumor Immunity: Potential Tumor-Induced Cytokines/Chemokines that Regulate TLS Formation in Epithelial-Derived Cancers

Feasibility of Unbiased RNA Profiling of Colorectal Tumors: A Proof of Principle

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