Gene expression and highly diluted molecules

Marzotto, Marta; Olioso, Debora; Bellavite, Paolo

doi:10.3389/fphar.2014.00237

Cited by 9 publications

(22 citation statements)

References 14 publications

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“…32 Anti-tumour homeopathic medicines induce expression changes in the whole genome of cancer cells. 33 These and other findings, reviewed elsewhere, [34][35][36][37][38][39] support the hypothesis that highly diluted homeopathic medicines are able to turn some important genes on or off, initiating a cascade of gene actions to correct the gene expression changes that produced the disorder or disease.…”

Section: Discussionmentioning

confidence: 60%

Fibronectin Gene Up-regulation by Arnica montana in Human Macrophages: Validation by Real-Time Polymerase Chain Reaction Assay

2020

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Background and Aim Arnica montana L. (Arnica m.) is a popular traditional medicine, used for its therapeutic properties in healing traumas, but little is known about its biological action on tissue formation and repair. This new work tested the effects of Arnica m. homeopathic dilutions on human macrophages, key cells in tissue defence and repair. Materials and Methods Macrophages derived from the THP-1 cell line were differentiated with interleukin-4 to induce a ‘wound-healing’-like phenotype, and treated with various dilutions of Arnica m. centesimal (100 times) dilutions (2c, 3c, 5c, 9c, and 15c) or control solvent for 24 hours. RNA samples from cultured cells were analysed by real-time quantitative polymerase chain reaction in five separate experiments. Results Arnica montana at the 2c dilution (final concentration of sesquiterpene lactones in cell culture = 10−8 mol/L) significantly stimulated the expression of three genes which code for regulatory proteins of the extracellular matrix, namely FN1 (fibronectin 1, % increase of 21.8 ± standard error of the mean 4.6), low-density lipoprotein-receptor-related protein 1 (% increase of 33.4 ± 6.1) and heparan sulphate proteoglycan 2 (% increase of 21.6 ± 9.1). Among these genes, the most quantitatively expressed was FN1. In addition, FN1, unlike other candidate genes, was upregulated in cells treated with higher dilutions/dynamisations (3c, 5c, and 15c) of Arnica m. Conclusion The results support evidence that the extracellular matrix is a potential therapeutic target of Arnica m., with positive effects on cell adhesion and migration during tissue development and healing.

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Section: Discussionmentioning

confidence: 60%

Fibronectin Gene Up-regulation by Arnica montana in Human Macrophages: Validation by Real-Time Polymerase Chain Reaction Assay

2020

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“…If we use generic DNA extracted from whole blood or any biological sample (auto-sarcode of general DNA), we would be acting on the epigenome systemic modulation, seeking to restore the organism's dynamic, complex, and global balance, either associated or not with chronic diseases. In turn, as long as it is feasible, by employing DNA extracted from specific tissues (auto-sarcode of specific DNA), we would be enhancing the response by directing gene modulation Marzotto et al 2014 38 Olioso et al 2014 39 Marzotto et al 2014 40 Bellavite et al 2018 41 Gelsemium toward epigenetic modifications that are located in particular tissues, which are responsible for manifesting and maintaining specific chronic diseases. Reiterating the possible therapeutic recommendations related to the homeopathic chronic miasms and their epigenetic representations, while the auto-sarcode of general DNA would act as anti-psoric medicine on the general epigenome modulation, the auto-sarcode of specific DNA would act as specific anti-miasmatic medicine by amplifying the epigenome modulation to the epigenetic alterations located in particular tissues.…”

Section: Isopathic Use Of Auto-sarcode Of Dna As Anti-miasmatic Homeomentioning

confidence: 99%

“…Marzotto et al 2014 38 Olioso et al 2014 39 Marzotto et al 2014 40 Bellavite et al 2018 41 Gelsemium by molecular biology laboratories following specific techniques and protocols 29,30 and later forwarded to pharmacies or homeopathic laboratories, so as to have auto-sarcode prepared following the homeopathic, pharmaco-technical method. 27 As an example of whole-blood general DNA extraction, the process is automated and uses specific methods and kits, 31 and the material is delivered in buffer solution (DNA dissolved in tris-EDTA), with concentration and purity informed.…”

Section: Isopathic Use Of Auto-sarcode Of Dna As Anti-miasmatic Homeomentioning

confidence: 99%

Isopathic Use of Auto-Sarcode of DNA as Anti-Miasmatic Homeopathic Medicine and Modulator of Gene Expression?

Teixeira

2019

Homeopathy

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Introduction In addition to the four pillars of homeopathy, vitalism and the miasmatic theory are often used to explain the health–disease process. According to Hahnemann's concepts, homeopathic miasms are the main obstacle to the cure of chronic diseases, with psora being the fundamental cause of all forms of diseases. According to modern genetics, the disease-promoting epigenetic alterations are the fundamental cause of the manifestation of chronic diseases. Objective This article develops a philosophical–scientific correlation between chronic miasms and disease-promoting epigenetic modifications, aiming to justify the isopathic use of auto-sarcode of an individual's DNA as homeopathic medicine. Results Based on the study of homeopathic doctrine and epigenetics, a conceptual and functional correlation is observed between homeopathic chronic miasms and disease-promoting epigenetic modifications. Additionally, several experimental studies suggest that homeopathy's mechanism of action may be by modulating gene expression. Conclusions By the philosophical–scientific correlations described, it is inferred that disease-promoting epigenetic alterations are the biological representation of the chronic miasms, suggesting the isopathic use of auto-sarcode of DNA as homeopathic therapeutic modulator of gene expression for the management of chronic diseases.

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“…The molar mass of ethanol solvent in tested solutions approaching a theoretical concentration of active principles lower than 1 attomole/L, is at least 16 orders of magnitude higher. As ethanol is a bioactive molecule at relatively low doses [18], its activity on gene expression may be highly complex and unpredictable, both in treated and control cells, so hindering a reliable statistical evaluation of the assay system.Some authors recently reported that “Perhaps we need to remind that normally, when one performs a study of dose–response and the concentration in the highest dose and the dilution factor are known, there is no way to determine the concentration of substances in all successive dilutions…” [19]. This suggests that the way Gelsemium in CAM approaches mood disorders and anxiety needs this standpoint to be addressed and further elucidated.…”

Section: Further Comments and Conclusive Remarksmentioning

confidence: 99%

On Gelsemium and Complementary and Alternative Medicine (CAM) in Anxiety and Experimental Neurology

Chirumbolo

2014

Neurol Ther

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A recent discussion expanded the debate about the experimental research on Gelsemium in anxiety. Herbal medicine is widely used in anxiety and mood disorders, often with contradictory evidence, although some authors are yet prompted to promote their full introduction in pharmacology as a promising therapy. Complementary and alternative medicine (CAM) in anxiety is particularly appreciated by individual healthcare, but deserves further investigation, as many critical issues have been recently raised. Comments about the ability of negligible doses of Gelsemium hydroalcoholic extracts to affect gene expression were recently reported.

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Gene expression and highly diluted molecules

Cited by 9 publications

References 14 publications

Fibronectin Gene Up-regulation by Arnica montana in Human Macrophages: Validation by Real-Time Polymerase Chain Reaction Assay

Fibronectin Gene Up-regulation by Arnica montana in Human Macrophages: Validation by Real-Time Polymerase Chain Reaction Assay

Isopathic Use of Auto-Sarcode of DNA as Anti-Miasmatic Homeopathic Medicine and Modulator of Gene Expression?

On Gelsemium and Complementary and Alternative Medicine (CAM) in Anxiety and Experimental Neurology

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