Gene Expression and Proteome Analysis as Sources of Biomarkers in Basal Cell Carcinoma

Lupu, Mihai; Căruntu, Constantin; Ghita, Mihaela; Voiculescu, Vlad Mihai; Voiculescu, Suzana Elena; Roşca, Adrian Eugen; Căruntu, Ana; Moraru, Liliana; Popa, Iris Maria; Calenic, Bogdan; Greabu, Maria; Costea, Daniela Elena

doi:10.1155/2016/9831237

Cited by 33 publications

(31 citation statements)

References 84 publications

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“…Pathogenesis of psoriasis and BCC is associated with keratinocytes hyperproliferation and their differentiation disorders, activation, adhesion, and migration of keratinocytes and endothelial cells, chronic inflammation, and angiogenesis [23,26,43,55,58]. The plasminogen system is known to be involved in all of these processes.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

et al. 2017

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There is substantial evidence implicating the urokinase system in tissue remodeling during neo-vascularization, inflammation, tumor invasion, and metastasis. Regulated degradation of the extracellular matrix at the leading edge of migrating cells, mediated by uPA and uPAR, is required for tissue remodeling, invasiveness, and angiogenesis. Psoriasis and basal cell carcinoma (BCC) are the most common skin diseases. Pathogenesis of both of them is associated with keratinocyte hyperproliferation, inflammatory cell migration, and angiogenesis-processes in which the plasminogen system (uPA, uPAR, tPA, and PAI-1) plays a crucial role. In the present study, the comparative analysis of uPA, uPAR, tPA, and PAI-1 expression in the normal skin, in the biopsies of patients with psoriasis vulgaris, and BCC was carried out. uPA, uPAR, and PAI-1 expression was up-regulated in the epidermis of psoriatic skin and in tumor cells in BCC. Increased uPAR expression was detected in the derma of psoriatic lesions and in the stroma surrounding tumor cells in BCC. Increased expression of uPA in epidermal cells in psoriasis and in tumor cells in BCC suggests an important role of the uPA system for aggressively proliferating and invading cells of epidermal origin. A possible activation of the stroma, as a result of uPA-uPAR interaction between tumor cells and the surrounding stroma, is suggested.

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Section: Discussionmentioning

confidence: 99%

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

et al. 2017

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“…Traditional analytical methods are not enough to elucidate complex biochemical processes or biological functions derived from the changes in protein profiles. Therefore, proteome methods coordinated with bioinformatics for data mining have provided a feasible tool for large-scale screening and differentially identifying protein targets that are linked to the pathogenesis and the pinpointing signaling pathways associated with UV-mediated skin damage [10][11][12]. Herein, we utilized MetaCore pathway software to comprehensively dissect the cellular pathways behind the differences in protein levels.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Biological Impact of UVA and UVB upon the Skin with Functional Proteomics and Immunohistochemistry

et al. 2019

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The skin provides protection against external stimuli; however, solar radiation, including ultraviolet A (UVA) and ultraviolet B (UVB), can result in profound influences on skin structure and function, which eventually impairs its molecular characteristics and normal physiology. In the current study, we performed proteome tools combined with an immunohistological approach on nude mouse skin to evaluate the adverse responses elicited by UVA and UVB irradiation, respectively. Our findings indicated that UVA significantly promotes oxidative damage in DNA, the breakdown of collagen fiber in the dermis, and the apoptosis of fibroblasts, which leads to inflammation. Meanwhile, UVB administration was found to enhance the carbonylation of various proteins and the proliferation of keratinocyte. Particularly, raspberry extract, which has been confirmed to have antioxidative efficacy, could effectively attenuate ultraviolet (UV) radiation-caused cell death. Network analysis also implied that UVA and UVB induce quite different responses, and that UVA results in cell death as well as inflammation mediated by caspase-3 and activator protein 1/nuclear factor kappa-light-chain-enhancer of activated B cells (AP-1/NF-κB), while UVB predominantly increases the risk of skin carcinogenesis involved with oncogenes such as p53 and c-Myc. Taken together, functional proteomics coordinated with histological experiments could allow for a high-throughput study to explore the alterations of crucial proteins and molecules linked to skin impacts subjected to UVA and UVB exposure.

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“…Squamous cell and basal carcinomas are common malignancies that entail several pitfalls in their treatment such as inadequate skin penetration of chemotherapic drugs and depth-related difficulties in excision [177][178][179][180][181][182]. Systemic application of cannabinoids was proven effective in inducing apoptosis and tumor growth inhibition in vitro and in vivo on PDV.C57 epidermal tumor models.…”

Section: Non-melanoma Cancersmentioning

confidence: 99%

Cannabinoids in the Pathophysiology of Skin Inflammation

et al. 2020

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Cannabinoids are increasingly-used substances in the treatment of chronic pain, some neuropsychiatric disorders and more recently, skin disorders with an inflammatory component. However, various studies cite conflicting results concerning the cellular mechanisms involved, while others suggest that cannabinoids may even exert pro-inflammatory behaviors. This paper aims to detail and clarify the complex workings of cannabinoids in the molecular setting of the main dermatological inflammatory diseases, and their interactions with other substances with emerging applications in the treatment of these conditions. Also, the potential role of cannabinoids as antitumoral drugs is explored in relation to the inflammatory component of skin cancer. In vivo and in vitro studies that employed either phyto-, endo-, or synthetic cannabinoids were considered in this paper. Cannabinoids are regarded with growing interest as eligible drugs in the treatment of skin inflammatory conditions, with potential anticancer effects, and the readiness in monitoring of effects and the facility of topical application may contribute to the growing support of the use of these substances. Despite the promising early results, further controlled human studies are required to establish the definitive role of these products in the pathophysiology of skin inflammation and their usefulness in the clinical setting.

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Gene Expression and Proteome Analysis as Sources of Biomarkers in Basal Cell Carcinoma

Cited by 33 publications

References 84 publications

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

Comparison of the Biological Impact of UVA and UVB upon the Skin with Functional Proteomics and Immunohistochemistry

Cannabinoids in the Pathophysiology of Skin Inflammation

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