Gene Expression Changes in Femoral Head Necrosis of Human Bone Tissue

Abstract

Abstract. Osteonecrosis of the femoral head (ONFH) is the result of an interruption of the local circulation and the injury of vascular supply of bone. Multiple factors have been implicated in the development of the disease. However the mechanism of ischemia and necrosis in non-traumatic ONFH is not clear. The aim of our investigation was to identify genes that are differently expressed in ONFH vs. non-ONFH human bone and to describe the relationships between these genes using multivariate data analysis. Six bone tissue samples from ONFH male patients and 8 bone tissue samples from non-ONFH men were examined. The expression differences of selected 117 genes were analyzed by TaqMan probe-based quantitative real-time RT-PCR system. The significance test indicated marked differences in the expression of nine genes between ONFH and non-ONFH individuals. These altered genes code for collagen molecules, an extracellular matrix digesting metalloproteinase, a transcription factor, an adhesion molecule, and a growth factor. Canonical variates analysis demonstrated that ONFH and non-ONFH bone tissues can be distinguished by the multiple expression profile analysis of numerous genes controlled via canonical TGFB pathway as well as genes coding for extracellular matrix composing collagen type molecules. The markedly altered gene expression profile observed in the ONFH of human bone tissue may provide further insight into the pathogenetic process of osteonecrotic degeneration of bone.

Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head

“…Recent evidence suggests that abnormalities in the bone metabolism pathway are implicated in its pathogenesis (Balla et al, 2011;Wen et al, 2012;Jiang et al, 2014).…”

Changed cellular functions and aberrantly expressed miRNAs and circRNAs in bone marrow stem�cells in osteonecrosis of the femoral head

“…Previous studies have demonstrated well that decreased proliferation capacity, enhanced apoptosis, impaired osteogenesis and enhanced adipogenesis were found in BMSCs of ONFH patients (3,4). In addition, aberrant gene expression was also found in BMScs of ONFH patients and thus, is considered as the major cause of the dysfunction of BMScs, particularly in the imbalance between osteogenesis and adipogenesis (5).…”

Genetic polymorphisms in plasminogen activator inhibitor-1 predict susceptibility to steroid-induced osteonecrosis of the femoral head in Chinese population

“…Non-traumatic osteonecrosis of femoral head (ONFH) represents an intractable bone disease, pathophysiologically characterized by progressive collapse of the femoral head because of a disturbance in the supply of blood and anomalies in the fibrinolytic system [1,2]. Since this ischemic necrosis of the femoral head and deterioration of hip joint function may significantly affect patient quality of life, it is urgent to clarify the pathogenic mechanisms regarding this disease.…”