Gene expression in early stage cervical cancer

Biewenga, Petra; Buist, Marrije R.; Moerland, Perry D.; Themaat, Emiel Ver Loren van; Kampen, Antoine H. C. van; Kate, Fiebo J.W. ten; Baas, Frank

doi:10.1016/j.ygyno.2007.11.024

Cited by 101 publications

(98 citation statements)

References 26 publications

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“…Immunohistochemistry is a cost-effective technique, but diverse epitopes (Cox-2, p16, CXCR4, CCR7, D2-40, etc) and controversial results were reported for the prediction of lymph node metastases in cervical cancer. 19 -24 Gene expression profiling studies also found divergent results with a study by Biewenga et al, 25 which was not able to predict lymph node metastases in early stage cervical cancer, while a study by Kim et al 26 did. Biewenga et al 25 found five genes showing differential expression between patients with and without lymph node metastases.…”

Section: Discussionmentioning

confidence: 99%

“…19 -24 Gene expression profiling studies also found divergent results with a study by Biewenga et al, 25 which was not able to predict lymph node metastases in early stage cervical cancer, while a study by Kim et al 26 did. Biewenga et al 25 found five genes showing differential expression between patients with and without lymph node metastases. The prediction of lymph node positivity will assist in developing adequate treatment options for patients since histologically undetectable micrometastases in the lymphatic system may account for cervical cancer recurrence.…”

Section: Discussionmentioning

confidence: 99%

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Fluorescence in Situ Hybridization Markers for Prediction of Cervical Lymph Node Metastases

Wangsa

Heselmeyer‐Haddad

Ried

et al. 2009

The American Journal of Pathology

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The presence of lymph node metastases is associated with poor prognosis in early stage cervical cancer. As of yet, no molecular markers predicting lymph node metastases have been identified. We examined single genetic markers and a composite marker, comprised of three fluorescence in situ hybridization (FISH) probes targeting the genes LAMP3, PROX1, and PRKAA1, in pretreatment cervical biopsies from 16 lymph node positive cases and 15 lymph node negative controls from women with stage IB and IIA cervical cancer. In addition, we determined clonal patterns by including CCND1 to compare the clonal constitution of primary tumors and associated lymph node metastases. The composite FISH marker allowed for classification of patients into those with and without lymph node metastases with a sensitivity and specificity of 75% and 87%, respectively (P ‫؍‬ 0.001). The positive predictive value and negative predictive value were 86% and 76% , respectively. Clonal patterns varied among the tumors. In many cases , changes between the primary tumor and lymph node metastases in the most common clones may indicate that certain clones have a growth advantage for establishing metastases in lymph nodes. We conclude that the composite FISH marker may be useful for determining risk for subsequent development of lymph node metastases in patients with cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fluorescence in Situ Hybridization Markers for Prediction of Cervical Lymph Node Metastases

Wangsa

Heselmeyer‐Haddad

Ried

et al. 2009

The American Journal of Pathology

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“…1, and data not shown). Compared with normal tissues, STX6 expression is elevated in breast, colon, liver, pancreatic, prostate, bladder, skin, testicular, tongue, cervical, lung and gastric cancers (Biewenga et al, 2008;D'Errico et al, 2009;Hou et al, 2010;Kaiser et al, 2007;Korkola et al, 2006;LaTulippe et al, 2002;Richardson et al, 2006;Riker et al, 2008;Sanchez-Carbayo et al, 2006;Segara et al, 2005;Wurmbach et al, 2007;Ye et al, 2008) (Fig. 1).…”

Section: Stx6 Is Overexpressed In Cancersmentioning

confidence: 95%

“…cDNA of human STX6 was amplified by PCR from an I.M.A.G.E. clone and cloned into the pCMV-3Tag-1a vector to generate a construct that encodes an (Richardson et al, 2006); 5 normal colon, 17 cecum adenocarcinoma (Kaiser et al, 2007); 10 normal liver, 35 hepatocellular carcinoma (Wurmbach et al, 2007); 6 normal pancreas, 11 pancreatic carcinoma (Segara et al, 2005); 3 normal prostate gland, 32 prostate carcinoma (LaTulippe et al, 2002); 48 normal bladder, 81 bladder urothelial carcinoma (Sanchez-Carbayo et al, 2006); 4 normal skin, 11 skin squamous cell carcinoma (Riker et al, 2008); 6 normal testis, 101 adult male germ cell tumor (Korkola et al, 2006); 12 normal tongue, 26 tongue squamous cell carcinoma (Ye et al, 2008); 5 normal cervix, 40 cervical squamous cell carcinoma (Biewenga et al, 2008); 65 normal lung, 27 squamous cell lung carcinoma (Hou et al, 2010); and 31 normal gastric mucosa, 4 gastric adenocarcinoma (D'Errico et al, 2009) samples was analyzed using microarrays. Dark gray boxes represent tumor tissues and light gray boxes represent normal tissues.…”

Section: Stx6 Overexpression Stimulates Cell Migration Spreading Andmentioning

confidence: 99%

Regulation of Integrin Endocytic Recycling and Chemotactic Cell Migration by Syntaxin 6 and VAMP3 Interaction

Riggs

Hasan

Humphrey

et al. 2012

Journal of Cell Science

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SummaryIntegrins are the primary receptors of cells adhering to the extracellular matrix, and play key roles in various cellular processes including migration, proliferation and survival. The expression and distribution of integrins at the cell surface is controlled by endocytosis and recycling. The present study examines the function of syntaxin 6 (STX6), a t-SNARE located in the trans-Golgi network, in integrin trafficking. STX6 is overexpressed in many types of human cancer. We show that depletion of STX6 inhibits chemotactic cell migration and the delivery of the laminin receptor a3b1 integrin to the cell surface, whereas STX6 overexpression stimulates chemotactic cell migration, integrin delivery, and integrin-initiated activation of focal adhesion kinase. These data indicate that STX6 plays a ratelimiting role in cell migration and integrin trafficking. In STX6-depleted cells, a3b1 integrin is accumulated in recycling endosomes that contain the v-SNARE VAMP3. Importantly, we show that STX6 and VAMP3 form a v-/t-SNARE complex, VAMP3 is required in a3b1 integrin delivery to the cell surface, and endocytosed a3b1 integrin traffics to both VAMP3 and STX6 compartments. Collectively, our data suggest a new integrin trafficking pathway in which endocytosed integrins are transported from VAMP3-containing recycling endosomes to STX6-containing trans-Golgi network before being recycled to the plasma membrane.

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“…De la même façon, la déplétion de LARP7 dans des cellules épithéliales mammaires en culture entraîne leur transformation, un processus qui requiert la hausse d'activité de P-TEFb [7]. La diminution de l'expression de LARP7 semble éga-lement être un bon marqueur prédictif de la présence de métastases ganglionnaires à un stade tumoral précoce [30]. L'activation de P-TEFb, qui résulte d'une déstabilisation de la RNP 7SK/HEXIM/P-TEFb, pourrait donc jouer un rôle important dans la prolifération et l'agressivité des cellules cancéreuses.…”

Section: Le Système De Régulation De P-tefb Cible Du Vih-1unclassified

Perturbations de la transcription liées à une dérégulation de P-TEFb : cancer, Sida et hypertrophie cardiaque

2012

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Gene expression in early stage cervical cancer

Cited by 101 publications

References 26 publications

Fluorescence in Situ Hybridization Markers for Prediction of Cervical Lymph Node Metastases

Fluorescence in Situ Hybridization Markers for Prediction of Cervical Lymph Node Metastases

Regulation of Integrin Endocytic Recycling and Chemotactic Cell Migration by Syntaxin 6 and VAMP3 Interaction

Perturbations de la transcription liées à une dérégulation de P-TEFb : cancer, Sida et hypertrophie cardiaque

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