Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer

Akram, Melakhessou Mohamed; Naima, Benkiki; Eddine, Marref Salah; Joubert, O.; Doumandji, Zahra; Safar, Ramia

doi:10.5530/jyp.2019.11.8

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Our previously reported data on A. flava verifies its anti-inflammatory, antipyretic, antioxidant, and anticancer potentials [6][7][8]. Some of the compounds present in the n-butanol extract of A. flava have already been confirmed to possess antidiabetic activities, for instance, the tiliroside and vicenin 3 [11].…”

Section: Discussionmentioning

confidence: 81%

“…(Asteraceae), locally called assenan aouragh in Algeria is one of the traditional medicinal plants which are widely distributed in the Mediterranean zone [5]. A.flava has shown significant antioxidant, anticancer, anti-inflammatory, and antipyretic activities [6][7][8]. Nonetheless, many health issues such as circulatory disorders, ulcers, intestinal parasites, and snakebite poisoning have been treated by a folk herbal drug made from Atractylis plants, while A. flava has also been reported to possess diuretic effects [9,10].…”

Section: Introductionmentioning

confidence: 99%

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In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

et al. 2021

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Background Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. Results The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. Conclusion BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

et al. 2021

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Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots

Maslov,

Komisarenko,

Kolisnyk

et al. 2023

Current Issues in Pharmacy and Medical Sciences

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The study has the intent of exploring the dynamics of biologically active substance (BAS) extractions from red raspberry (R. idaeus) shoots in order to determine the appropriate extraction frequency. A secondary objective is to assess the relationship between extract antioxidant activity and BAS content. The focus of the study revolved around R. idaeus shoots, and the spectrophotometric method of analysis was applied to determine the amount of the catechins, polyphenols, hydroxycinnamic acids derivatives and flavonoids, while the gravimetric method was used to determine the dry residue of the extracts and the potentiometric method applied for evaluating antioxidant activity. The polyphenols amount was 1.74±0.03, 0.58±0.01 and 0.016±0.005%, catechins – 1.50±0.02, 0.50±0.01 and 0.11±0.01%, flavonoids – 0.08±0.005, 0.046±0.005 and 0.016±0.002%, hydroxycinnamic acids derivatives – 0.24±0.005, 0.078±0.005 and 0.017±0.002%, organic acids – 0.15±0.005, 0.05±0.005 and 0.01±0.002, dry residue – 2.14±0.005, 0.72±0.01 and 0.16±0.005%, antioxidant activity – 94.80±1.90, 47.40±0.95 and 7.90±0.16 mM-eqv./m for the 1st, 2nd and 3rd extraction, respectively. The optimal extraction frequency of raspberry shoots with distilled water was two times. The highest value of Peasorn‘s correlation coefficient was between the antioxidant activity and catechins (r = 0.9813) and the lowest in the case of organic acids (r=0.9112). The appropriate extraction frequency of R. idaeus shoots with distilled water was two times. The antioxidant method of determination extraction frequency was not inferior to the traditional method. The catechins dominated in the extracts from R. idaeus shoots. A significant positive correlation was content of catechins and antioxidant activity.

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The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells

Melakhessou,

Eddine,

Zahra

et al. 2024

Current Issues in Pharmacy and Medical Sciences

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Atractylis flava Desf. (AF) is common plant that is widely used for its anti- inflammatory and antioxidant properties. The purpose of this study was, therefore, to evaluate the cytotoxic effect and the molecular basis of antioxidant and anti-inflammatory effects of the ethyl acetate extract (AFEAE) obtained from the whole plant A. flava. This was accomplished through the use of NR8383 alveolar rat macrophage cells. Cultures of alveolar rat macrophage cells were treated with AFEAE (25–800 μg/mL), and cell viability was determined via WST-1 and LDH tests. In turn, the gene expression levels of nuclear factor κB (NF-κB), tumor necrosis factor-alpha (TNFα), interleukin 1 beta (IL1-β), interleukin 6 (IL-6), mitochondrial dynamin like GTPase (OPA1), Succinate dehydrogenase complex subunit A (SDHA) and neutrophil cytosolic factor 1 (NCF1) were assessed by applying RT-qPCR. The results show that ethyl acetate extracts of A. flava have non-cytotoxic effects, and the gene expression analysis demonstrates that AFEAF extracts generate significant downregulation of NF-κB, TNFα, IL-1 β, IL-6, NCF1, OPA1 and SDHA, compared to untreated cells. This study reveals that Atractylis flava ethyl acetate extract administration may be considered as a potential therapeutic strategy for inflammatory related diseases.

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Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer

Cited by 4 publications

References 12 publications

In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots

The effect of ethyl acetate extract from Atractylis flava Desf. on the gene expression of pro-inflammatory cytokines and oxidative stress markers in NR8383 alveolar rat macrophage cells

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