Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Huang, Kun; Li, Jin; Ito, Mikako; Takeda, Jun‐ichi; Ohkawara, Bisei; Ogi, Tomoo; Matsubara, Akio; Ohno, Kinji

doi:10.3389/fnmol.2020.00154

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…IP assays of HNSCC cells from K14CreER;RiboTag;Mettl1 fl/fl or K14CreER;RiboTag;Mettl1 wt/wt mice were performed as described previously [ 38 , 39 ]. Briefly, HNSCC tissues were mashed by a multisample tissue grinding machine (Jingxin, Xinchang, Zhejiang, China) in supplemental homogenization buffer.…”

Section: Methodsmentioning

confidence: 99%

Aberrant translation regulated by METTL1/WDR4‐mediated tRNA N7‐methylguanosine modification drives head and neck squamous cell carcinoma progression

Chen

Wang

et al. 2022

Cancer Communications

111

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Background: Cancer cells selectively promote the translation of oncogenic transcripts to stimulate cancer progression. Although growing evidence has revealed that tRNA modifications and related genes participate in this process, their roles in head and neck squamous cell carcinoma (HNSCC) remain largely uncharacterized. Here, we sought to investigate the function and mechanisms of the transfer RNA (tRNA) N7-methylguanosine (m 7 G) modification in regulating the occurrence and development of HNSCC.

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Section: Methodsmentioning

confidence: 99%

Aberrant translation regulated by METTL1/WDR4‐mediated tRNA N7‐methylguanosine modification drives head and neck squamous cell carcinoma progression

Chen

Wang

et al. 2022

Cancer Communications

111

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“…The muscle and nerve samples were immediately frozen in isopentane, cooled with liquid nitrogen, and stored at −80°C. Immunohistochemical staining was performed as described elsewhere, with minor modifications 10–12 . Briefly, histological and immunohistochemical analyses were carried out on 8‐μm‐thick frozen sections prepared using a cryostat.…”

Section: Methodsmentioning

confidence: 99%

Clinicopathological features of titinopathy from a Chinese neuromuscular center

Huang

Duan

et al. 2021

Neuropathology

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Titin, one of the largest proteins in humans, is a major component of muscle sarcomeres. Pathogenic variants in the titin gene (TTN) have been reported to cause a range of skeletal muscle diseases, collectively known as titinopathy. Titinopathy is a heterogeneous group of disabling diseases characterized by muscle weakness. In our study, we aimed to establish the clinicopathologicalgenetic spectrum of titinopathy from a single neuromuscular center. Three patients were diagnosed as having definite titinopathy, and additional three patients were diagnosed as having possible titinopathy according to the diagnostic criteria. All the patients showed initial symptoms from age one to 40 years. Physical examination revealed that five patients had muscle weakness, and that one patient experienced behavioral changes. Muscle biopsy specimens obtained from all six patients demonstrated multiple myopathological changes, including increased fiber size variation, muscle fiber hypertrophy or atrophy, formation of centralized cell nuclei, necklace cytoplasmic bodies, and formation of rimmed vacuoles and cores. Genetic testing revealed 11 different TTN alterations, including missense (6/11), nonsense (2/11), frameshift (2/ 11), and splicing (1/11) mutations. Our study provides further evidence that TTN mutations are more likely to be responsible for an increasing proportion of various myopathies, such as hereditary myopathy with early respiratory failure (HMERF), core myopathy, and distal myopathy with rimmed vacuoles, than currently recognized mutations. Our findings expand the clinical, pathohistological and genetic spectrum of titinopathy.

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“…The results of this study were analysed and reported in accordance with the STROBE guidelines 8 . The real-time PCR test was performed as previously described with modification 9 , 10 , and was based on the criteria provided by the World Health Organization (WHO) 11 . Nasopharyngeal swabs were taken on the 1st, 7th and 14th days of medical isolation observation.…”

Section: Methodsmentioning

confidence: 99%

Repositive RT-PCR test in discharged COVID-19 patients during medical isolation observation

Huang¹,

Li²,

Zhou³

et al. 2021

Int. J. Med. Sci.

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Objectives: The epidemiological and clinical characteristics of patients with coronavirus disease 2019 (COVID-19) have been researched. However, the prevalence of repositivity by real-time PCR for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains unclear. Methods: A retrospective study was conducted involving 599 discharged patients with COVID-19 in a single medical centre. The clinical features of patients during their hospitalization and 14-day post-discharge quarantine were collected. Results: A total of 122 patients (20.4%) out of 599 patients retested positive after discharge. Specifically, 94 (15.7%) retested positive within 24 h of discharge, and another 28 patients (4.7%) were repositive on day 7 after discharge, although none showed any clinical symptomatic recurrence. Both repositives and non‑repositives have similar patterns of IgG and IgM. Notably, the length of hospitalization of non-repositive patients was longer than that of 24-h repositive patients and 7-day repositive patients. In addition, the length of hospitalization of 24-h repositive patients was shorter than that of 7-day repositive patients, indicating that the length of hospitalization was also a determinant of viral shedding. Conclusion: Our study provides further information for improving the management of recovered and discharged patients, and further studies should be performed to elucidate the infectiveness of individuals with prolonged or RNA repositivity.

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Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Cited by 14 publications

References 39 publications

Aberrant translation regulated by METTL1/WDR4‐mediated tRNA N7‐methylguanosine modification drives head and neck squamous cell carcinoma progression

Aberrant translation regulated by METTL1/WDR4‐mediated tRNA N7‐methylguanosine modification drives head and neck squamous cell carcinoma progression

Clinicopathological features of titinopathy from a Chinese neuromuscular center

Repositive RT-PCR test in discharged COVID-19 patients during medical isolation observation

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