Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle

Sugasawa, Takehito; Komine, Ritsuko; Manevich, Lev; Tamai, Shinsuke; Takekoshi, Kazuhiro; Kanki, Yasuharu

doi:10.3390/nu14112239

Cited by 3 publications

(3 citation statements)

References 47 publications

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“…Fasting and refeeding are used to investigate the biological response of teleosts [ 37 ]. In the present study, the GIPR mRNA level in the kidney and fat tissue of grass carp was markedly induced by fasting for 14 days.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

Yang

Liang

Jiang

et al. 2022

Aquaculture Nutrition

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GIP plays an important regulatory role in glucose and lipid metabolism. As the specific receptor, GIPR is involved in this physiological process. To assess the roles of GIPR in teleost, the GIPR gene was cloned from grass carp. The ORF of cloned GIPR gene was 1560 bp, encoding 519 amino acids. The grass carp GIPR was the G-protein-coupled receptor which contains seven predicted transmembrane domains. In addition, two predicted glycosylation sites were contained in the grass carp GIPR. The grass carp GIPR expression is in multiple tissues and is highly expressed in the kidney, brain regions, and visceral fat tissue. In the OGTT experiment, the GIPR expression is markedly decreased in the kidney, visceral fat, and brain by treatment with glucose for 1 and 3 h. In the fast and refeeding experiment, the GIPR expression in the kidney and visceral fat tissue was significantly induced in the fast groups. In addition, the GIPR expression levels were markedly decreased in the refeeding groups. In the present study, the visceral fat accumulation of grass carp was induced by overfed. The GIPR expression was significantly decreased in the brain, kidney, and visceral fat tissue of overfed grass carp. In primary hepatocytes, the GIPR expression was promoted by treatment with oleic acid and insulin. The GIPR mRNA levels were significantly reduced by treatment with glucose and glucagon in the grass carp primary hepatocytes. To our knowledge, this is the first time the biological role of GIPR is unveiled in teleost.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

Yang

Liang

Jiang

et al. 2022

Aquaculture Nutrition

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“…Total RNA-Seq was performed to identify the novel RNA markers, that is, fluctuated expressions dependent on the blood storage period. The method was similar to procedures in our previous study [ 23 , 24 ]. Preparation of the libraries and NGS run was conducted at the Department of Sports Medicine in the Organization for Open Facility Initiatives, University of Tsukuba (Tsukuba, Ibaraki, Japan).…”

Section: Methodsmentioning

confidence: 99%

Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion

Sugasawa

Kanki

Komine

et al. 2022

Genes

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The World Anti-Doping Agency (WADA) has prohibited the use of autologous blood transfusion (ABT) as a doping method by athletes. It is difficult to detect this doping method in laboratory tests, and a robust testing method has not yet been established. We conducted an animal experiment and used total RNA sequencing (RNA-Seq) to identify novel RNA markers to detect ABT doping within red blood cells (RBCs) as a pilot study before human trials. This study used whole blood samples from Wistar rats. The whole blood samples were mixed with a citrate–phosphate–dextrose solution with adenine (CPDA) and then stored in a refrigerator at 4 °C for 0 (control), 10, or 20 days. After each storage period, total RNA-Seq and bioinformatics were performed following RNA extraction and the purification of the RBCs. In the results, clear patterns of expression fluctuations were observed depending on the storage period, and it was found that there were large numbers of genes whose expression decreased in the 10- and 20-day periods compared to the control. Moreover, additional bioinformatic analysis identified three significant genes whose expression levels were drastically decreased according to the storage period. These results provide novel insights that may allow future studies to develop a testing method for ABT doping.

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“…The messenger RNA was sequenced at the Department of Sports Medicine (Open Facility Network Office, University of Tsukuba, Tsukuba, Japan). A cDNA library prepared as described in [24] was sequenced using a Next Seq 500 system (Illumina Inc., San Diego, CA, USA). We mapped FASTQ files to the A. nidulans genome (https://fungidb.org/fungidb/app/record/dataset/DS_4b35c88aa1, accessed on 6 January 2023) and the gene expression was evaluated as transcripts per kilobase million (TPM) using CLC Genomics Workbench 20.0.4 (Qiagen).…”

Section: Sequencing Mrnamentioning

confidence: 99%

The Metabolic Regulation of Amino Acid Synthesis Counteracts Reactive Nitrogen Stress via Aspergillus nidulans Cross-Pathway Control

Amahisa,

Tsukagoshi,

Kadooka

et al. 2024

JoF

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Nitric oxide (NO) is a natural reactive nitrogen species (RNS) that alters proteins, DNA, and lipids and damages biological activities. Although microorganisms respond to and detoxify NO, the regulation of the cellular metabolic mechanisms that cause cells to tolerate RNS toxicity is not completely understood. We found that the proline and arginine auxotrophic proA5 and argB2 mutants of the fungus Aspergillus nidulans require more arginine and proline for normal growth under RNS stress that starves cells by accumulating fewer amino acids. Fungal transcriptomes indicated that RNS stress upregulates the expression of the biosynthetic genes required for global amino acids, including proline and arginine. A mutant of the gene disruptant, cpcA, which encodes the transcriptional regulation of the cross-pathway control of general amino acid synthesis, did not induce these genes, and cells accumulated fewer amino acids under RNS stress. These results indicated a novel function of CpcA in the cellular response to RNS stress, which is mediated through amino acid starvation and induces the transcription of genes for general amino acid synthesis. Since CpcA also controls organic acid biosynthesis, impaired intermediates of such biosynthesis might starve cells of amino acids. These findings revealed the importance of the mechanism regulating amino acid homeostasis for fungal responses to and survival under RNS stress.

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Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle

Cited by 3 publications

References 47 publications

Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

Molecular Characterization of Grass Carp GIPR and Effect of Nutrition States, Insulin, and Glucagon on Its Expression

Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion

The Metabolic Regulation of Amino Acid Synthesis Counteracts Reactive Nitrogen Stress via Aspergillus nidulans Cross-Pathway Control

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