Gene expression profiles in mouse cumulus cells derived from in vitro matured oocytes with and without blastocyst formation

Xu, Yixin; Zhou, Tuanping; Li, Shao; Zhang, Bei; Liu, Kailu; Gao, Chao; Gao, Li; Liu, Jiayin; Cui, Yugui; Chian, Ri‐Cheng

doi:10.1016/j.gep.2017.05.002

Cited by 10 publications

(4 citation statements)

References 67 publications

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“…For example, Hu et al [14] reported the cause of insulin resistance in polycystic ovarian syndrome (PCOS) analyzing cumulus cells. Xu et al [15] searched the difference in gene expression of mouse cumulus cells with and without blastocyst formation. Fuchs et al [16] studied the difference in the gene expression of human cumulus granulosa cells based on the types of drugs used to trigger IVF cycles.…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

et al. 2020

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Background: Dysmature cumulus cells are lower fertilization rates and abnormalities in embryonic development compared to maturation cumulus cells. Morphological evaluation of cumulus–oocyte complexes (COCs) considered the possibility that differences may also be found in gene expression. Purpose: To identify hallmarks for evaluating oocyte quality by investigating gene expression patterns in human cumulus cells surrounding oocytes. Methods: Cumulus cells were obtained from the cumulus–oocyte complex of infertile women treated with assisted reproductive technology. Based on maturity level, the cumulus cells were classified into two categories, i.e., dysmature cumulus cell (DCC) and maturation cumulus cell. DCCs were subjected to gene expression analysis using next-generation sequencing and compared with COCs that are in the process of maturation as controls. Results: The expression levels of genes involved in the Wnt signal/β-catenin pathway were significantly reduced in DCCs compared with those in control cells. Moreover, the expression levels of genes involved in multiple pathways associated with apoptosis were also significantly reduced compared with those in control cells. Conclusions: DCCs showed significant decreases in apoptosis- and Wnt/β-catenin signaling-associated gene expression. DCCs could be classified by morphological evaluation, and the method described in this study may be useful as an oocyte quality estimation tool.

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Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

et al. 2020

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“…In this context, the NSUN4 gene has been reported to be differentially expressed in mouse oocytes. NSUN4 is a candidate gene involved in the association with cumulus cells, responsible for oocyte viability and embryonic developmental competence [ 38 ], which could possibly explain their association with AFC in the current study.…”

Section: Discussionmentioning

confidence: 95%

“…Due to the large number of genes in the region of these HapAlleles and a involvement of some of them in various metabolic pathways, we have discussed only those that have been previously described in association with reproductive traits, as well as performed by Xu et al [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

et al. 2018

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Zebu cattle (Bos taurus indicus) are highly adapted to tropical regions. However, females reach puberty after taurine heifers, which affects the economic efficiency of beef cattle breeding in the tropical regions. The aims of this study were to establish associations between the haplotype alleles of the bovine genome and age at first calving (AFC) in the Nelore cattle, and to identify the genes and quantitative trait loci (QTL) related to this phenotype. A total of 2,273 Nelore cattle (995 males and 1,278 females) genotyped using the Illumina BovineHD BeadChip were used in the current study. The association analysis included females with valid first calving records as well as open heifers. Linkage disequilibrium (LD) analysis among the markers was performed using blocks of 5, 10, and 15 markers, which were determined by sliding windows shifting one marker at a time. Then, the haplotype block size to be used in the association study was chosen based on the highest r2 average among the SNPs in the block. The five HapAlleles most strongly associated with the trait (top five) were considered as significant associations. The results of the analysis revealed four genomic regions related to AFC, which overlapped with 20 QTL of the reproductive traits reported previously. Furthermore, there were 19 genes related to reproduction in those regions. In conclusion, the use of haplotypes allowed the detection of chromosomal regions associated with AFC in Nelore cattle, and provided the basis for elucidating the mechanisms underlying this trait.

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“…Subsequently, the oocytes were placed into 100 μl of M2 droplets in a petri dish (35 × 10 mm, Corning Incorporated, Corning, NY, USA) and incubated in a CO 2 incubator for 30 min before fertilization procedure. The protocol was the same as before which was mentioned by Prof. RC Chian [12].…”

Section: Cryopreservation Of Oocytesmentioning

confidence: 99%

Enhancement of the efficiency of oocyte vitrification through regulation of histone deacetylase 6 expression

Wang

Zhang

Zhao

et al. 2018

J Assist Reprod Genet

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Gene expression profiles in mouse cumulus cells derived from in vitro matured oocytes with and without blastocyst formation

Cited by 10 publications

References 67 publications

Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

Next-Generation Sequencing Reveals Downregulation of the Wnt Signaling Pathway in Human Dysmature Cumulus Cells as a Hallmark for Evaluating Oocyte Quality

Genome-wide association study using haplotype alleles for the evaluation of reproductive traits in Nelore cattle

Enhancement of the efficiency of oocyte vitrification through regulation of histone deacetylase 6 expression

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