Gene expression profiles reveal that chondrogenic progenitor cells and synovial cells are closely related

Zhou, Cheng; Zhang, Hongjun; Seol, Dongrim; Yu, Yancun; Martin, James A.

doi:10.1002/jor.22641

Cited by 39 publications

(41 citation statements)

References 24 publications

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“…These fi ndings together with data that primary synovial chondromatosis nodules contained cells that spontaneously differentiated along chondrogenic and osteogenic lineages [30] and that the gene expression profi les of chondrogenic progenitor cells and synovial cells are closely related [31] indicate that SM and SF contain cells that could be designated as mesenchymal chondro-osteogenic or chondrogenic progenitors. Da bi utvrdili da li sinovijalna tečnost (ST) pasa sadrži ćelije koje imaju karakteristike mezenhinskih matičnih ćelija (MMĆ), uzorkovana je ST od 5 mladih pasa (starosti 3.8 ± 0.9 godina).…”

Section: Discussionmentioning

confidence: 75%

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

et al. 2015

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The aim of this study was to determine whether synovial fl uid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 ± 0.9 years) were expanded (37ºC, 5% CO 2 , humidifi ed atmosphere) three weeks before their phenotype was characterized by fl ow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 ± 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 ± 134. At P1, 0.55 ± 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets' slides have shown a signifi cant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.

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Section: Discussionmentioning

confidence: 75%

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

et al. 2015

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“…While adult AC is considered an avascular, aneural, and alymphatic tissue with little capacity for self-repair after injury, several studies have identified chondrogenic progenitor cells in the superficial zone of normal and osteoarthritic AC in animals and humans 51, 62, 64–67 . However, the function of the progenitor cells in AC repair during the adult stage needs to be further elucidated.…”

Section: The Link Between Development and Regenerationmentioning

confidence: 99%

“…Moreover, synovial MSCs express a specific proteoglycan (superficial zone protein), a functional characteristic of progenitor cells in the superficial zone of AC. Gene expression profiles revealed that chondrogenic progenitor cells from the superficial zone of AC and synovial cells are closely related 67, 77–80 . Thus, synovial MSCs may be particularly useful in regenerating the superficial layer of AC.…”

Section: The Link Between Development and Regenerationmentioning

confidence: 99%

Cell-based articular cartilage repair: the link between development and regeneration

Caldwell

Wang

2015

Osteoarthritis and Cartilage

145

111

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Context Clinical efforts to repair damaged articular cartilage (AC) currently face major obstacles due to limited intrinsic repair capacity of the tissue and unsuccessful biological interventions. This highlights a need for better therapeutic strategies. Evidence Acquisition Relevant articles were identified through a search of the PubMed database from January 1956 to August 2014 using the following keywords: articular cartilage repair, stem cell, cartilage tissue-engineering, synovium, and NFAT. Evidence Synthesis In both animals and humans, AC defects that penetrate into the subchondral bone marrow are mainly filled with fibrocartilaginous tissue through the differentiation of bone marrow mesenchymal stem cells (MSCs), followed by degeneration of repaired cartilage and osteoarthritis. Cell therapy and tissue engineering techniques using culture-expanded chondrocytes, bone marrow MSCs, or pluripotent stem cells with chondroinductive growth factors may generate cartilaginous tissue in AC defects but do not form hyaline cartilage-based articular surface because repair cells often lose chondrogenic activity or result in chondrocyte hypertrophy. The new evidence that AC and synovium develop from the same pool of precursors with similar gene profiles and that synovium-derived chondrocytes have stable chondrogenic activity has promoted use of synovium as a new cell source for AC repair. The recent finding that NFAT1 and NFAT2 transcription factors inhibit chondrocyte hypertrophy and maintain metabolic balance in AC is a significant advance in the field of AC repair. Conclusions The use of synovial MSCs and discovery of upstream transcriptional regulators that help maintain the AC phenotype have opened new avenues to improve the outcome of AC regeneration.

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“…Both processes are beaten because the tissue engineered cartilage matrix secreted by SDSCs is rich in collagen-II and aggrecan but not collagen-I or collagen-X and is mechanically similar to articular cartilage [64].…”

Section: Synovium-derived Mscsmentioning

confidence: 99%

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fuentes-Mera¹,

Camacho²,

Moncada‐Saucedo³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Articular cartilage injuries caused by traumatic/mechanical progressive degeneration result in joint pain, swelling, the consequent loss of joint function, and eventually osteoarthritis. Articular tissue possesses a poor ability to regenerate that further complicates the therapeutic approaches. Mesenchymal stem cells (MSCs) have emerged as a promising alternative treatment. Recently, it has been reported that a wide variety of strategies ranging from merely using cells in the injured area to employ biofunctional substitutes in which cells are harmonizing with scafolding and growth factors to create an engineered cartilage tissue.This chapter reviews the state-of the-art in cartilage tissue engineering focused on tissue engineering approaches designed to recapitulate the native development of cartilage and its tridimensional structure as an osteochondral unit. Since the production of hypertrophied tissue is one of the most critical challenges to overcome in chondral tissue regeneration, here we show new strategies to minimize hypertrophy in cartilage. Finally, the eicacy and safety of diferent treatments of cartilage in current clinical trials will be discussed.While the framework provides new features and beneits concerning the strategies for articular tissue regeneration, this chapter presents a set of tools to improve approaches to orthopedic regenerative medicine based on the use of MSCs.Keywords: MSCs, MSCs-subpopulations, cartilage regeneration, cartilage tissue engineering, hypertrophy © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionThe chondrocytes are the only cells found in cartilage. The chondrocytes demonstrate distinctive properties such as being metabolically active in order to maintain the renewal of the extracellular matrix (ECM) by synthesizing collagens, proteoglycans, hyaluronic acid, and glycoproteins. Restoration of the cartilage damage is still challenging for orthopedic medicine due to its poor ability to regenerate [1].Mesenchymal stem cells (MSCs) have potential applications in tissue engineering, and regenerative medicine represents an atractive option for repairing lesions in cartilage. Stem cellbased therapies that harmonize with tissue-engineering technologies, and biomaterials are vital for the continuous advance of cartilage regenerative medicine [2,3].Once the relationship between structure function in normal and damaged tissues is understood and the development of biological substitutes for the repair or regeneration can be reached. To develop a biological substitute, tissue engineering uses scafolds, cells, and growth factors. Each of these elements alone is able to promote tissue regeneration, but composites fabricated in combination would be more efective [4,5].The objective of the present chapter is, therefore, t...

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Gene expression profiles reveal that chondrogenic progenitor cells and synovial cells are closely related

Cited by 39 publications

References 24 publications

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

Proliferation And Differentiation Potential Of Canine Synovial Fluid Cells

Cell-based articular cartilage repair: the link between development and regeneration

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

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