Gene expression profiling in gill tissues of White spot syndrome virus infected black tiger shrimp Penaeus monodon by DNA microarray

Abstract

White spot syndrome virus (WSSV) continues to be the most devastating viral pathogen infecting penaeid shrimp the world over. The genome of WSSV has been deciphered and characterized from three geographical isolates and significant progress has been made in developing various molecular diagnostic methods to detect the virus. However, the information on host immune gene response to WSSV pathogenesis is limited. Microarray analysis was carried out as an approach to analyse the gene expression in black tiger shrimp Penaeus monodon in response to WSSV infection. Gill tissues collected from the WSSV infected shrimp at 6, 24, 48 h and moribund stage were analysed for differential gene expression. Shrimp cDNAs of 40,059 unique sequences were considered for designing the microarray chip. The Cy3-labeled cRNA derived from healthy and WSSV-infected shrimp was subjected to hybridization with all the DNA spots in the microarray which revealed 8,633 and 11,147 as up- and down-regulated genes respectively at different time intervals post infection. The altered expression of these numerous genes represented diverse functions such as immune response, osmoregulation, apoptosis, nucleic acid binding, energy and metabolism, signal transduction, stress response and molting. The changes in gene expression profiles observed by microarray analysis provides molecular insights and framework of genes which are up- and down-regulated at different time intervals during WSSV infection in shrimp. The microarray data was validated by Real Time analysis of four differentially expressed genes involved in apoptosis (translationally controlled tumor protein, inhibitor of apoptosis protein, ubiquitin conjugated enzyme E2 and caspase) for gene expression levels. The role of apoptosis related genes in WSSV infected shrimp is discussed herein.

A new insight to biomarkers related to resistance in survived-white spot syndrome virus challenged giant tiger shrimp,Penaeus monodon

“…Various techniques have been applied to study shrimp immune responses to viral infections, including cDNA microarray technology (Aoki et al, 2011; Lu, Hsiao & Wu, 2011; Shekhar et al, 2015) and next generation sequencing (Clavero-Salas et al, 2007; Zeng et al, 2013; Chen et al, 2013). Few researches involving transcriptome study with WSSV infection in shrimp and prawn have been performed to understand the effect of WSSV infection to the host immune system.…”

Identification of Functional Gene Modules Associated With STAT-Mediated Antiviral Responses to White Spot Syndrome Virus in Shrimp

“…They found that penaeidin-3 isoforms and crustin were over-expressed in hemocytes of WSSV-infected pre-challenged Penaeus vannamei ( García et al, 2009 ). Shekhar et al (2015) utilized DNA microarray technology to explore the genes of host immune responses to WSSV pathogenesis. They found some up- or down-regulated genes during WSSV infection in shrimp ( Shekhar et al, 2015 ).…”

“… Shekhar et al (2015) utilized DNA microarray technology to explore the genes of host immune responses to WSSV pathogenesis. They found some up- or down-regulated genes during WSSV infection in shrimp ( Shekhar et al, 2015 ). Yu et al (2017) found that a combination of single nucleotide polymorphisms in three genes (TRAF6, Cu/Zn SOD, and nLvALF2) were significantly associated with resistance to WSSV infection.…”

Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon

“…Several methods including expressed sequenced tag (EST) 7–9 , DNA microarray 10–14 and proteomic 15–19 , have been used to analyse molecular changes during WSSV infection.…”

“…Despite intensive investigations of WSSV infection, the mechanisms of WSSV entry and propagation in shrimp have not yet been fully understood. Several methods including expressed sequenced tag (EST) 7 – 9 , DNA microarray 10 – 14 and proteomic 15 – 19 , have been used to analyse molecular changes during WSSV infection.…”