Gene expression profiling in porcine mammary gland during lactation and identification of breed-and developmental-stage-specific genes

Su, Zhixi; Dong, Xiaoyan; Zhang, Bing; Zeng, Yanwu; Fu, Yan; Yu, Jun; Hu, Songnian

doi:10.1007/s11427-005-0181-0

Cited by 10 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several of the top lactation-associated genes are related to fatty acid metabolisms, in agreement with the results of research conducted on porcine mammary gland development [3]. One example is Cidea, which regulates lipolysis through the negative control of tumor necrosis factor-alpha (TNF-alpha) transcription in human adipocytes [37] and possibly perhaps via monitoring of lipid droplet size [38].…”

Section: Decisive Changes In Both Hk and Ms Gene Regulation During Thsupporting

confidence: 78%

“…Linnaeus, recognizing their uniqueness in that female mammals nurse their young with milk produced by the mammary glands, classified the mammary-gland-bearing animals as belonging to the class Mammalia [2]. As one of the mammal-specific organs, the mammary gland has been widely studied at the molecular level [3], with many studies being micro-array-based [46]. For example, Clarkson et al [5] performed microarray studies and identified the difference in gene expression over 12 time points during mouse mammogenesis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic analysis reveals key regulators of mammogenesis and the pregnancy-lactation cycle

Zhou

Gong

Xiao

et al. 2014

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

An organ unique to mammals, the mammary gland develops 90% of its mass after birth and experiences the pregnancy-lactation-involution cycle (PL cycle) during reproduction. To understand mammogenesis at the transcriptomic level and using a ribo-minus RNA-seq protocol, we acquired greater than 50 million reads each for the mouse mammary gland during pregnancy (day 12 of pregnancy), lactation (day 14 of lactation), and involution (day 7 of involution). The pregnancy-, lactation-and involution-related sequencing reads were assembled into 17344, 10160, and 13739 protein-coding transcripts and 1803, 828, and 1288 non-coding RNAs (ncRNAs), respectively. Differentially expressed genes (DEGs) were defined in the three samples, which comprised 4843 DEGs (749 up-regulated and 4094 down-regulated) from pregnancy to lactation and 4926 DEGs (4706 up-regulated and 220 down-regulated) from lactation to involution. Besides the obvious and substantive upand down-regulation of the DEGs, we observe that lysosomal enzymes were highly expressed and that their expression coincided with milk secretion. Further analysis of transcription factors such as Trps1, Gtf2i, Tcf7l2, Nupr1, Vdr, Rb1, and Aebp1, Let7,, and mir-15 has enabled us to identify key regulators in mammary gland development and the PL cycle. mouse mammary gland, mammogenesis, transcriptome, rmRNA-seq, miRNAs, transcription factors Citation:

show abstract

Section: Decisive Changes In Both Hk and Ms Gene Regulation During Thsupporting

confidence: 78%

mentioning

confidence: 99%

Transcriptomic analysis reveals key regulators of mammogenesis and the pregnancy-lactation cycle

Zhou

Gong

Xiao

et al. 2014

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ESTs will be a valuable source for many researchers working with tumor tissue [8] . These ESTs, especially specific for canine mammary gland tissue, provide significant information for canine mammary cancer studies.…”

Section: Discussionmentioning

confidence: 99%

“…The mammary gland is an excellent system to study questions about organogenesis, cell differentiation and oncogenesis [8] . The most commonly diagnosed neoplasm in female dogs is mammary-gland tumor which is of an epithelial origin, and approximately 50% are malignant [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Terrier Köpeklerinin Normal ve Tümörlü Meme Bezi Dokusunda Eksprese Edilen Genlerin Birincil Analizi

Özgentürk¹,

Ulu²,

Ulu³

et al. 2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog could be a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, which were collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonies which are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST data were analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences were grouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on gene homology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs. Keywords: Terrier, Dog, Breast cancer, EST, cDNA library Terrier Köpeklerinin Normal ve Tümörlü Meme Bezi Dokusunda Eksprese Edilen Genlerin Birincil Analizi ÖzMeme bezi tümörü, dişi köpeklerde en sık rastlanan tümör türüdür. Tümör oluşumu ve tümör oluşum mekanizması ile ilgili genler hakkındaki veriler yetersizdir. Köpekteki tümörigenez çalışmalarının insan meme bezi tümörleri için bir model olabileceğini görmek için karşılaştırmalı çalışmalar yürütülmüştür. Bu çalışmada, sağlıklı bir Terrier köpeğinin normal meme dokusundan ve hasta bir köpekten tümörlü meme dokusundan toplanan iki farklı dokudan iki farklı cDNA kütüphanesi oluşturuldu. Bir köpek meme bezi EST kolleksiyonu geliştirmek için, iki kütüphaneden rastgele seçilen toplam 2304 koloni dizilendi. Ham EST verileri Phred/Phrap programları ile analiz edildi ve okunabilir EST dizileri CAP3 programı ile bir araya getirildi. Tüm EST dizileri 45 kontig ve 2203 singlet grubuna ayrıldı. Tüm benzersiz sekansların varsayımsal fonksiyonları, NCBI BLAST tarafından gen homolojisine dayalı olarak belirlendi ve BLAST2GO ile açıklandı. Bu çalışmanın sonuçları, köpeklerin fonksiyonel genom çalışmaları için çok değerli bir kaynaktır.

show abstract

“…Due to the high level of protein synthesis in the lactation phase, expression of the proteins that participate in ribosomal structure increases at the preparation lactation stage. Also, the genes that have function in transcriptional and translational regulation are expressed highly before parturition [27]. Additionally, we have observed known putative genes related to immune system, growth, and lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep

Özgentürk

Ulu

et al. 2017

International Journal of Genomics

View full text Add to dashboard Cite

Kivircik sheep is an important local Turkish sheep according to its meat quality and milk productivity. The aim of this study was to analyze gene expression profiles of both prenatal and postnatal stages for the Kivircik sheep. Therefore, two different cDNA libraries, which were taken from the same Kivircik sheep mammary gland tissue at prenatal and postnatal stages, were constructed. Total 3072 colonies which were randomly selected from the two libraries were sequenced for developing a sheep ESTs collection. We used Phred/Phrap computer programs for analysis of the raw EST and readable EST sequences were assembled with the CAP3 software. Putative functions of all unique sequences and statistical analysis were determined by Geneious software. Total 422 ESTs have over 80% similarity to known sequences of other organisms in NCBI classified by Panther database for the Gene Ontology (GO) category. By comparing gene expression profiles, we observed some putative genes that may be relative to reproductive performance or play important roles in milk synthesis and secretion. A total of 2414 ESTs have been deposited to the NCBI GenBank database (GW996847–GW999260). EST data in this study have provided a new source of information to functional genome studies of sheep.

show abstract

Gene expression profiling in porcine mammary gland during lactation and identification of breed-and developmental-stage-specific genes

Cited by 10 publications

References 28 publications

Transcriptomic analysis reveals key regulators of mammogenesis and the pregnancy-lactation cycle

Transcriptomic analysis reveals key regulators of mammogenesis and the pregnancy-lactation cycle

Terrier Köpeklerinin Normal ve Tümörlü Meme Bezi Dokusunda Eksprese Edilen Genlerin Birincil Analizi

Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep

Contact Info

Product

Resources

About